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Showing 1 to 8 of 8 entries
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Reversibly soluble biocatalyst: optimization of trypsin coupling to Eudragit S-100 and biocatalyst activity in soluble and precipitated forms.

Enzyme and microbial technology

Arasaratnam V, Galaev IY, Mattiasson B.
PMID: 10899551
Enzyme Microb Technol. 2000 Aug 01;27(3):254-263. doi: 10.1016/s0141-0229(00)00195-2.

Eudragit S-100, a copolymer of methacrylic acid and methyl methacrylate is soluble at pH above 5 and insoluble at pH below 4.5. pH-dependent solubility of the polymer is used for the development of reversibly soluble biocatalyst, which combines the...

Highly Sensitive Novel Biosensor Based on an Immobilized lac Repressor.

Angewandte Chemie (International ed. in English)

Bontidean I, Kumar A, Csöregi E, Galaev IY, Mattiasson B.
PMID: 29712325
Angew Chem Int Ed Engl. 2001 Jul 16;40(14):2676-2678. doi: 10.1002/1521-3773(20010716)40:14<2676::AID-ANIE2676>3.0.CO;2-5.

Sensitive repressors: The conformational change that occurs in the lac repressor protein when it binds to an inducer molecule has been used to develop a biosensor that permits the detection of the corresponding operator sequence or specific inducer molecules....

Affinity thermoprecipitatin: Contribution of the efficiency of ligand-protein interaction and access of the ligand.

Biotechnology and bioengineering

Galaev IY, Mattiasson B.
PMID: 18601296
Biotechnol Bioeng. 1993 May;41(11):1101-6. doi: 10.1002/bit.260411113.

Conjugates to two thermoprecipitating polymers, poly(N-vinyl caprolactam) and poly(N-isopropylacrylmide), with soybean trypsin inhibitor, Cibacron Blue 3GA, Cu-iminodiacetic acid, and p-aminobenzamidine were synthesized. The interaction of these conjugates with trypsin and lactate dehydrogenase was studied. Coupling of the ligand to...

Affinity precipitation of alpha-amylase inhibitor from wheat meal by metal chelate affinity binding using cu(II)-loaded copolymers of 1-vinylimidazole with N-isopropylacrylamide .

Biotechnology and bioengineering

Galaev IY, Kumar A, Mattiasson B.
PMID: 10099390
Biotechnol Bioeng. 1998 Sep 20;59(6):695-704. doi: 10.1002/(sici)1097-0290(19980920)59:6<695::aid-bit6>3.0.co;2-a.

A method for purifying alpha-amylase inhibitor from wheat meal based on immobilized metal affinity with a thermosensitive copolymer is developed. The studies represent the thermoprecipitation properties of the copolymers of N-isopropylacrylamide (NIPAM) with iminodiacetic acid (IDA) and 1-vinylimidazole (VI),...

Ultrafast Responsive Poly- (N-isopropylacrylamide) Gel Produced by Cryostructuring of Self-crosslinkable Polymer Microgels.

Macromolecular rapid communications

Kirsebom H, Mattiasson B, Galaev IY.
PMID: 21590861
Macromol Rapid Commun. 2010 Jun 16;31(12):1095-100. doi: 10.1002/marc.201000015. Epub 2010 May 17.

A macroporous material composed of closely aggregated particles was prepared by cryo-structuration of N-isopropylacrylamide-co-N-hydroxymethylacrylamide (NIPA-co-HMAm) particle suspensions. The formed structure was maintained by the formation of covalent bonds through self-crosslinking between the particles while the system was in a...

Thermosensitive, Reversibly Cross-Linking Gels with a Shape "Memory".

Angewandte Chemie (International ed. in English)

Samra BK, Galaev IY, Mattiasson B.
PMID: 10941093
Angew Chem Int Ed Engl. 2000 Jul 03;39(13):2364-2367. doi: 10.1002/1521-3773(20000703)39:13<2364::aid-anie2364>3.0.co;2-j.

No abstract available.

Highly Sensitive Novel Biosensor Based on an Immobilized lac Repressor This work was supported with grants given by the Swedish Engineering Research Council (TFR). Prof. Muller Hill and Dr. Andrew Barker are acknowledged for providing plasmids and the Escherichia coli BMH 8117 strain. .

Angewandte Chemie (International ed. in English)

Bontidean I, Kumar A, Csöregi E, Galaev IY, Mattiasson B.
PMID: 11458370
Angew Chem Int Ed Engl. 2001 Jul 16;40(14):2676-2678.

No abstract available.

Cryogels from poly(2-hydroxyethyl methacrylate): macroporous, interconnected materials with potential as cell scaffolds.

Soft matter

Savina IN, Cnudde V, D'Hollander S, Van Hoorebeke L, Mattiasson B, Galaev IY, Du Prez F.
PMID: 32900039
Soft Matter. 2007 Aug 14;3(9):1176-1184. doi: 10.1039/b706654f.

Macroporous hydrogels (MHs) have been prepared by cross-linking polymerization of hydroxyethyl methacrylate (HEMA) or cross-linking co-polymerization of HEMA with dimethylacrylamide (DMAA) in semi-frozen state. The MHs are elastic and have a unique structure of large interconnected pores with pore...

Showing 1 to 8 of 8 entries