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Showing 25 to 36 of 121 entries
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New and improved HPLC methods for drug formulations and clinical analysis.

Journal of AOAC International

Agbaba D, Sherma J.
PMID: 20922934
J AOAC Int. 2010 Jul-Aug;93(4):1057-8.

No abstract available.

Simultaneous determination of ten active components in 12 Chinese Piper species by HPLC.

The American journal of Chinese medicine

Cai CC, Yan MC, Xie H, Pan SL.
PMID: 21905291
Am J Chin Med. 2011;39(5):1043-60. doi: 10.1142/S0192415X11009391.

Piper is a genus that is recently valued for the treatment of central nervous system diseases. The major constituents, amides and lignans, are responsible for the antinociceptive and antidepressant activities. This study developed a RP-HPLC-UV method for the simultaneous...

Improved liquid chromatography fingerprint of fat-soluble Radix isatidis extract using multi-wavelength combination technique.

Journal of separation science

Zhou W, Xie MF, Zhang XY, Liu TT, Yu YJ, Duan GL.
PMID: 21442749
J Sep Sci. 2011 May;34(10):1123-32. doi: 10.1002/jssc.201000834. Epub 2011 Mar 25.

We first investigated liquid chromatography (LC) fingerprint method using multi-wavelength combination technique, and successfully used this method for the analysis of a fat-soluble extract from Radix isatidis. LC fingerprints of fat-soluble R. isatidis extracts from 11 origins were established...

Simultaneous quantitative determination of six active components in traditional Chinese medicinal preparation Cerebralcare Granule® by RP-HPLC coupled with diode array detection for quality control.

Journal of chromatographic science

Wang XY, Ma XH, Li W, Chu Y, Guo JH, Zhou SP, Zhu YH.
PMID: 23934038
J Chromatogr Sci. 2014 Sep;52(8):814-7. doi: 10.1093/chromsci/bmt120. Epub 2013 Aug 08.

A simple, accurate and reliable method for the simultaneous separation and determination of six active components (protocatechuic acid, chlorogenic acid, caffeic acid, paeoniflorin, ferulic acid and rosmarinic acid) in traditional Chinese medicinal preparation Cerebralcare Granule(®) (CG) was developed using...

Effect of extra-column volume on practical chromatographic parameters of sub-2-μm particle-packed columns in ultra-high pressure liquid chromatography.

Journal of separation science

Wu N, Bradley AC, Welch CJ, Zhang L.
PMID: 22761164
J Sep Sci. 2012 Aug;35(16):2018-25. doi: 10.1002/jssc.201200074. Epub 2012 Jul 04.

Effects of extra-column volume on apparent separation parameters were studied in ultra-high pressure liquid chromatography with columns and inlet connection tubings of various internal diameters (id) using 50-mm long columns packed with 1.8-μm particles under isocratic conditions. The results...

Qualitative and quantitative determination of nine main active constituents in Pulsatilla cernua by high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry.

Journal of separation science

Xu H, Shi X, Ji X, Du Y, Zhu H, Zhang L.
PMID: 21268254
J Sep Sci. 2011 Feb;34(3):308-16. doi: 10.1002/jssc.201000660. Epub 2011 Jan 03.

A novel qualitative and quantitative method using high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) was developed for simultaneous determination of the nine major active constituents in Pulsatilla cernua (Thunb.) Bercht. et Opiz., namely anemoside A3...

[Quality evaluation of wildlife tending Polygonum perfoliatum].

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica

Tian L, Chen H, Zhou X, Tan J, Xue J.
PMID: 22803356
Zhongguo Zhong Yao Za Zhi. 2012 May;37(9):1180-3.

OBJECTIVE: To evaluate the quality of Polygonum perfoliatum collected from GAP planting base efficiently so as to provide scientific evidence for its GAP tending plant by HPLC fingerprinting method.METHOD: Analysis was performed on a reserved-phase column Lichrosher-C18 column (4.6...

[HPLC fingerprint of Euodiae Fructus].

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica

Zhao P, Xu H, Xu J, Zhang T, Zhu X.
PMID: 21355244
Zhongguo Zhong Yao Za Zhi. 2010 Dec;35(23):3178-83.

OBJECTIVE: To establish a HPLC fingerprint for quality evaluation of fructus Euodiae rutaecarpa, and to perform qualitative and quantitative analysis of its main constituents.METHOD: The 10 batches of samples were analyzed by HPLC linear gradient elution method and evaluated...

[Multi-component quantitative method of Chinese herbal additives based on fingerprint].

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica

Luo J, Cai J, Xiao X, Fu C, Liao K, Wang Y, Luo G.
PMID: 21837968
Zhongguo Zhong Yao Za Zhi. 2011 May;36(10):1295-7.

OBJECTIVE: To establish a full-quantified fingerprint method for analyzing Chinese herbal additives by HPLC.METHOD: A HPLC in combination with PDA detector was applied with a phenomenex luna C18 (4.6 mm x 250 mm, 5 microm) column by gradient elution...

[Analysis on chemical components of Ligustrum lucidum by ultra performance liquid chromatography-electrospray ionization-quadrupole-time of flight-mass spectrometry].

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica

Jiang Y, Yao W, Zhang L, Ding A.
PMID: 23189739
Zhongguo Zhong Yao Za Zhi. 2012 Aug;37(15):2304-8.

OBJECTIVE: To make a qualitative analysis on chemical components in Ligustrum lucidum by UPLC-ESI-Q-TOF-MS.METHOD: ACQUITY UPLC BEH C18 (2.1 mm x 100 mm, 1.7 microm) column was adopted, with methyl cyanides-0.1% formic acid as the mobile phase for gradient...

Expanding the term "Design Space" in high performance liquid chromatography (I).

Journal of pharmaceutical and biomedical analysis

Monks KE, Rieger HJ, Molnár I.
PMID: 21893394
J Pharm Biomed Anal. 2011 Dec 15;56(5):874-9. doi: 10.1016/j.jpba.2011.04.015. Epub 2011 Apr 22.

The current article presents a novel approach to applying Quality by Design (QbD) principles to the development of high pressure reversed phase liquid chromatography (HPLC) methods. Four common critical parameters in HPLC--gradient time, temperature, pH of the aqueous eluent,...

Ion-pair reversed-phase chromatography of short double-stranded deoxyribonucleic acid in silicon micro-pillar array columns: retention model and applications.

Journal of chromatography. A

Zhang L, Majeed B, Lagae L, Peumans P, Van Hoof C, De Malsche W.
PMID: 23647613
J Chromatogr A. 2013 Jun 14;1294:1-9. doi: 10.1016/j.chroma.2013.04.002. Epub 2013 Apr 08.

Separation of double-stranded (ds) DNAs is important in numerous biochemical analyses relevant for clinical applications. A widely used separation technique is high performance liquid chromatography (HPLC), in the variant of ion-pair reversed-phase (IP-RP) chromatography. HPLC can be miniaturized by...

Showing 25 to 36 of 121 entries