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Impact of Tenofovir-Based Pre-exposure Prophylaxis on Biomarkers of Bone Formation, Bone Resorption, and Bone Mineral Metabolism in HIV-Negative Adults.

Open forum infectious diseases

Nickolas TL, Yin MT, Hong T, Mugwanya KK, Branch AD, Heffron R, Ramalho J, Nandakumar R, Dworakowski E, Wanga V, Mugo NR, Ronald A, Celum C, Donnell D, Baeten JM, Wyatt CM.
PMID: 31660332
Open Forum Infect Dis. 2019 Jul 28;6(10):ofz338. doi: 10.1093/ofid/ofz338. eCollection 2019 Oct.

BACKGROUND: Pre-exposure prophylaxis (PrEP) with emtricitabine (FTC)/tenofovir disoproxil fumarate (TDF) reduces the risk of HIV seroconversion but may promote bone mineral density (BMD) decline. The mechanisms of BMD decline with FTC/TDF remain unclear, and studies in HIV-positive individuals have...

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Nickolas TL, Yin MT, Hong T, et al. Impact of Tenofovir-Based Pre-exposure Prophylaxis on Biomarkers of Bone Formation, Bone Resorption, and Bone Mineral Metabolism in HIV-Negative Adults. Open Forum Infect Dis. 2019;6(10):ofz338doi: 10.1093/ofid/ofz338.
Nickolas, T. L., Yin, M. T., Hong, T., Mugwanya, K. K., Branch, A. D., Heffron, R., Ramalho, J., Nandakumar, R., Dworakowski, E., Wanga, V., Mugo, N. R., Ronald, A., Celum, C., Donnell, D., Baeten, J. M., Wyatt, C. M. (2019). Impact of Tenofovir-Based Pre-exposure Prophylaxis on Biomarkers of Bone Formation, Bone Resorption, and Bone Mineral Metabolism in HIV-Negative Adults. Open forum infectious diseases, 6(10), ofz338. https://doi.org/10.1093/ofid/ofz338
Nickolas, Thomas L, et al. "Impact of Tenofovir-Based Pre-exposure Prophylaxis on Biomarkers of Bone Formation, Bone Resorption, and Bone Mineral Metabolism in HIV-Negative Adults." Open forum infectious diseases vol. 6,10 (2019): ofz338. doi: https://doi.org/10.1093/ofid/ofz338
Nickolas TL, Yin MT, Hong T, Mugwanya KK, Branch AD, Heffron R, Ramalho J, Nandakumar R, Dworakowski E, Wanga V, Mugo NR, Ronald A, Celum C, Donnell D, Baeten JM, Wyatt CM. Impact of Tenofovir-Based Pre-exposure Prophylaxis on Biomarkers of Bone Formation, Bone Resorption, and Bone Mineral Metabolism in HIV-Negative Adults. Open Forum Infect Dis. 2019 Jul 28;6(10):ofz338. doi: 10.1093/ofid/ofz338. eCollection 2019 Oct. PMID: 31660332; PMCID: PMC6778426.
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Rectal organoid morphology analysis (ROMA) as a promising diagnostic tool in cystic fibrosis.

Thorax

Cuyx S, Ramalho AS, Corthout N, Fieuws S, Fürstová E, Arnauts K, Ferrante M, Verfaillie C, Munck S, Boon M, Proesmans M, Dupont L, De Boeck K, Vermeulen F.
PMID: 33859053
Thorax. 2021 Nov;76(11):1146-1149. doi: 10.1136/thoraxjnl-2020-216368. Epub 2021 Apr 15.

Diagnosing cystic fibrosis (CF) when sweat chloride is not in the CF range and less than 2 disease-causing

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Cuyx S, Ramalho AS, Corthout N, et al. Rectal organoid morphology analysis (ROMA) as a promising diagnostic tool in cystic fibrosis. Thorax. 2021;76(11):1146-1149doi: 10.1136/thoraxjnl-2020-216368.
Cuyx, S., Ramalho, A. S., Corthout, N., Fieuws, S., Fürstová, E., Arnauts, K., Ferrante, M., Verfaillie, C., Munck, S., Boon, M., Proesmans, M., Dupont, L., De Boeck, K., Vermeulen, F. (2021). Rectal organoid morphology analysis (ROMA) as a promising diagnostic tool in cystic fibrosis. Thorax, 76(11), 1146-1149. https://doi.org/10.1136/thoraxjnl-2020-216368
Cuyx, Senne, et al. "Rectal organoid morphology analysis (ROMA) as a promising diagnostic tool in cystic fibrosis." Thorax vol. 76,11 (2021): 1146-1149. doi: https://doi.org/10.1136/thoraxjnl-2020-216368
Cuyx S, Ramalho AS, Corthout N, Fieuws S, Fürstová E, Arnauts K, Ferrante M, Verfaillie C, Munck S, Boon M, Proesmans M, Dupont L, De Boeck K, Vermeulen F. Rectal organoid morphology analysis (ROMA) as a promising diagnostic tool in cystic fibrosis. Thorax. 2021 Nov;76(11):1146-1149. doi: 10.1136/thoraxjnl-2020-216368. Epub 2021 Apr 15. PMID: 33859053.
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Protocol for Application, Standardization and Validation of the Forskolin-Induced Swelling Assay in Cystic Fibrosis Human Colon Organoids.

STAR protocols

Vonk AM, van Mourik P, Ramalho AS, Silva IAL, Statia M, Kruisselbrink E, Suen SWF, Dekkers JF, Vleggaar FP, Houwen RHJ, Mullenders J, Boj SF, Vries R, Amaral MD, de Boeck K, van der Ent CK, Beekman JM.
PMID: 33111074
STAR Protoc. 2020 Jun 03;1(1):100019. doi: 10.1016/j.xpro.2020.100019. eCollection 2020 Jun 19.

This protocol describes the isolation, handling, culture of, and experiments with human colon stem cell organoids in the context of cystic fibrosis (CF). In human colon organoids, the function of cystic fibrosis transmembrane conductance regulator (CFTR) protein and its...

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Vonk AM, van Mourik P, Ramalho AS, et al. Protocol for Application, Standardization and Validation of the Forskolin-Induced Swelling Assay in Cystic Fibrosis Human Colon Organoids. STAR Protoc. 2020;1(1):100019doi: 10.1016/j.xpro.2020.100019.
Vonk, A. M., van Mourik, P., Ramalho, A. S., Silva, I. A. L., Statia, M., Kruisselbrink, E., Suen, S. W. F., Dekkers, J. F., Vleggaar, F. P., Houwen, R. H. J., Mullenders, J., Boj, S. F., Vries, R., Amaral, M. D., de Boeck, K., van der Ent, C. K., & Beekman, J. M. (2020). Protocol for Application, Standardization and Validation of the Forskolin-Induced Swelling Assay in Cystic Fibrosis Human Colon Organoids. STAR protocols, 1(1), 100019. https://doi.org/10.1016/j.xpro.2020.100019
Vonk, Annelotte M, et al. "Protocol for Application, Standardization and Validation of the Forskolin-Induced Swelling Assay in Cystic Fibrosis Human Colon Organoids." STAR protocols vol. 1,1 (2020): 100019. doi: https://doi.org/10.1016/j.xpro.2020.100019
Vonk AM, van Mourik P, Ramalho AS, Silva IAL, Statia M, Kruisselbrink E, Suen SWF, Dekkers JF, Vleggaar FP, Houwen RHJ, Mullenders J, Boj SF, Vries R, Amaral MD, de Boeck K, van der Ent CK, Beekman JM. Protocol for Application, Standardization and Validation of the Forskolin-Induced Swelling Assay in Cystic Fibrosis Human Colon Organoids. STAR Protoc. 2020 Jun 03;1(1):100019. doi: 10.1016/j.xpro.2020.100019. eCollection 2020 Jun 19. PMID: 33111074; PMCID: PMC7580120.
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Bio-inspired gold microtubes based on the morphology of filamentous fungi.

Biomaterials science

Fontes AM, Geris R, Dos Santos AV, Pereira MG, Ramalho JGS, da Silva AF, Malta M.
PMID: 32481969
Biomater Sci. 2014 Jul 03;2(7):956-960. doi: 10.1039/c4bm00030g. Epub 2014 Mar 25.

This communication describes a general method for templating fungal filaments with gold nanoparticles that results in a gold replica of filaments after calcination of the biological template.

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Fontes AM, Geris R, Dos Santos AV, et al. Bio-inspired gold microtubes based on the morphology of filamentous fungi. Biomater Sci. 2014;2(7):956-960doi: 10.1039/c4bm00030g.
Fontes, A. M., Geris, R., Dos Santos, A. V., Pereira, M. G., Ramalho, J. G. S., da Silva, A. F., & Malta, M. (2014). Bio-inspired gold microtubes based on the morphology of filamentous fungi. Biomaterials science, 2(7), 956-960. https://doi.org/10.1039/c4bm00030g
Fontes, Adriana M, et al. "Bio-inspired gold microtubes based on the morphology of filamentous fungi." Biomaterials science vol. 2,7 (2014): 956-960. doi: https://doi.org/10.1039/c4bm00030g
Fontes AM, Geris R, Dos Santos AV, Pereira MG, Ramalho JGS, da Silva AF, Malta M. Bio-inspired gold microtubes based on the morphology of filamentous fungi. Biomater Sci. 2014 Jul 03;2(7):956-960. doi: 10.1039/c4bm00030g. Epub 2014 Mar 25. PMID: 32481969.
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Experiences of parent-adolescent dyads regarding a Facebook-based intervention to improve overweight/obesity treatment in adolescents: A qualitative study.

Applied psychology. Health and well-being

Félix S, Ramalho S, Ribeiro E, Pinheiro J, de Lourdes M, Gonçalves S, Conceição E.
PMID: 34286907
Appl Psychol Health Well Being. 2021 Jul 19; doi: 10.1111/aphw.12294. Epub 2021 Jul 19.

APOLO-Teens is a Facebook-based psychological intervention aiming to optimize hospital treatment for adolescents with overweight/obesity. The present qualitative study aims to explore (1) how adolescents experienced participation in APOLO-Teens and (2) how parents perceived adolescents' participation. Sixteen adolescent-parent dyads...

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Félix S, Ramalho S, Ribeiro E, et al. Experiences of parent-adolescent dyads regarding a Facebook-based intervention to improve overweight/obesity treatment in adolescents: A qualitative study. Appl Psychol Health Well Being. 2021;doi: 10.1111/aphw.12294.
Félix, S., Ramalho, S., Ribeiro, E., Pinheiro, J., de Lourdes, M., Gonçalves, S., & Conceição, E. (2021). Experiences of parent-adolescent dyads regarding a Facebook-based intervention to improve overweight/obesity treatment in adolescents: A qualitative study. Applied psychology. Health and well-being, . https://doi.org/10.1111/aphw.12294
Félix, Sílvia, et al. "Experiences of parent-adolescent dyads regarding a Facebook-based intervention to improve overweight/obesity treatment in adolescents: A qualitative study." Applied psychology. Health and well-being vol. (2021). doi: https://doi.org/10.1111/aphw.12294
Félix S, Ramalho S, Ribeiro E, Pinheiro J, de Lourdes M, Gonçalves S, Conceição E. Experiences of parent-adolescent dyads regarding a Facebook-based intervention to improve overweight/obesity treatment in adolescents: A qualitative study. Appl Psychol Health Well Being. 2021 Jul 19; doi: 10.1111/aphw.12294. Epub 2021 Jul 19. PMID: 34286907.
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Synergy in Cystic Fibrosis Therapies: Targeting SLC26A9.

International journal of molecular sciences

Pinto MC, Quaresma MC, Silva IAL, Railean V, Ramalho SS, Amaral MD.
PMID: 34884866
Int J Mol Sci. 2021 Dec 02;22(23). doi: 10.3390/ijms222313064.

SLC26A9, a constitutively active Cl

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Pinto MC, Quaresma MC, Silva IAL, et al. Synergy in Cystic Fibrosis Therapies: Targeting SLC26A9. Int J Mol Sci. 2021;22(23)doi: 10.3390/ijms222313064.
Pinto, M. C., Quaresma, M. C., Silva, I. A. L., Railean, V., Ramalho, S. S., & Amaral, M. D. (2021). Synergy in Cystic Fibrosis Therapies: Targeting SLC26A9. International journal of molecular sciences, 22(23), . https://doi.org/10.3390/ijms222313064
Pinto, Madalena C, et al. "Synergy in Cystic Fibrosis Therapies: Targeting SLC26A9." International journal of molecular sciences vol. 22,23 (2021). doi: https://doi.org/10.3390/ijms222313064
Pinto MC, Quaresma MC, Silva IAL, Railean V, Ramalho SS, Amaral MD. Synergy in Cystic Fibrosis Therapies: Targeting SLC26A9. Int J Mol Sci. 2021 Dec 02;22(23). doi: 10.3390/ijms222313064. PMID: 34884866; PMCID: PMC8658147.
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Influence of 35% hydrogen peroxide gel renewal on color change during in-office dental photobleaching with violet LED: A split-mouth randomized controlled clinical trial.

Photodiagnosis and photodynamic therapy

Youssef SA, Cunha SR, Mayer-Santos E, Brito SA, de Freitas PM, Ramalho J, Morimoto S, Gonçalves F, Ramalho KM.
PMID: 34455106
Photodiagnosis Photodyn Ther. 2021 Dec;36:102509. doi: 10.1016/j.pdpdt.2021.102509. Epub 2021 Aug 26.

BACKGROUND: To clinically evaluate the effect of 35% hydrogen peroxide gel renewal in association with violet LED (405-410nm) through a split-mouth randomized controlled clinical trial.METHODS: The treatment consisted in 3 bleaching sessions of 15 min each, with an interval...

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Youssef SA, Cunha SR, Mayer-Santos E, et al. Influence of 35% hydrogen peroxide gel renewal on color change during in-office dental photobleaching with violet LED: A split-mouth randomized controlled clinical trial. Photodiagnosis Photodyn Ther. 2021;36:102509doi: 10.1016/j.pdpdt.2021.102509.
Youssef, S. A., Cunha, S. R., Mayer-Santos, E., Brito, S. A., de Freitas, P. M., Ramalho, J., Morimoto, S., Gonçalves, F., & Ramalho, K. M. (2021). Influence of 35% hydrogen peroxide gel renewal on color change during in-office dental photobleaching with violet LED: A split-mouth randomized controlled clinical trial. Photodiagnosis and photodynamic therapy, 36102509. https://doi.org/10.1016/j.pdpdt.2021.102509
Youssef, Shady A, et al. "Influence of 35% hydrogen peroxide gel renewal on color change during in-office dental photobleaching with violet LED: A split-mouth randomized controlled clinical trial." Photodiagnosis and photodynamic therapy vol. 36 (2021): 102509. doi: https://doi.org/10.1016/j.pdpdt.2021.102509
Youssef SA, Cunha SR, Mayer-Santos E, Brito SA, de Freitas PM, Ramalho J, Morimoto S, Gonçalves F, Ramalho KM. Influence of 35% hydrogen peroxide gel renewal on color change during in-office dental photobleaching with violet LED: A split-mouth randomized controlled clinical trial. Photodiagnosis Photodyn Ther. 2021 Dec;36:102509. doi: 10.1016/j.pdpdt.2021.102509. Epub 2021 Aug 26. PMID: 34455106.
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Insights into HLA-G Genetics Provided by Worldwide Haplotype Diversity.

Frontiers in immunology

Castelli EC, Ramalho J, Porto IO, Lima TH, Felício LP, Sabbagh A, Donadi EA, Mendes-Junior CT.
PMID: 25339953
Front Immunol. 2014 Oct 06;5:476. doi: 10.3389/fimmu.2014.00476. eCollection 2014.

Human leukocyte antigen G (HLA-G) belongs to the family of non-classical HLA class I genes, located within the major histocompatibility complex (MHC). HLA-G has been the target of most recent research regarding the function of class I non-classical genes....

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Castelli EC, Ramalho J, Porto IO, et al. Insights into HLA-G Genetics Provided by Worldwide Haplotype Diversity. Front Immunol. 2014;5:476doi: 10.3389/fimmu.2014.00476.
Castelli, E. C., Ramalho, J., Porto, I. O., Lima, T. H., Felício, L. P., Sabbagh, A., Donadi, E. A., & Mendes-Junior, C. T. (2014). Insights into HLA-G Genetics Provided by Worldwide Haplotype Diversity. Frontiers in immunology, 5476. https://doi.org/10.3389/fimmu.2014.00476
Castelli, Erick C, et al. "Insights into HLA-G Genetics Provided by Worldwide Haplotype Diversity." Frontiers in immunology vol. 5 (2014): 476. doi: https://doi.org/10.3389/fimmu.2014.00476
Castelli EC, Ramalho J, Porto IO, Lima TH, Felício LP, Sabbagh A, Donadi EA, Mendes-Junior CT. Insights into HLA-G Genetics Provided by Worldwide Haplotype Diversity. Front Immunol. 2014 Oct 06;5:476. doi: 10.3389/fimmu.2014.00476. eCollection 2014. PMID: 25339953; PMCID: PMC4186343.
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Rescue of Mutant CFTR Trafficking Defect by the Investigational Compound MCG1516A.

Cells

Lopes-Pacheco M, Bacalhau M, Ramalho SS, Silva IAL, Ferreira FC, Carlile GW, Thomas DY, Farinha CM, Hanrahan JW, Amaral MD.
PMID: 35011698
Cells. 2022 Jan 01;11(1). doi: 10.3390/cells11010136.

Although some therapeutic progress has been achieved in developing small molecules that correct F508del-CFTR defects, the mechanism of action (MoA) of these compounds remain poorly elucidated. Here, we investigated the effects and MoA of MCG1516A, a newly developed F508del-CFTR...

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Lopes-Pacheco M, Bacalhau M, Ramalho SS, et al. Rescue of Mutant CFTR Trafficking Defect by the Investigational Compound MCG1516A. Cells. 2022;11(1)doi: 10.3390/cells11010136.
Lopes-Pacheco, M., Bacalhau, M., Ramalho, S. S., Silva, I. A. L., Ferreira, F. C., Carlile, G. W., Thomas, D. Y., Farinha, C. M., Hanrahan, J. W., & Amaral, M. D. (2022). Rescue of Mutant CFTR Trafficking Defect by the Investigational Compound MCG1516A. Cells, 11(1), . https://doi.org/10.3390/cells11010136
Lopes-Pacheco, Miquéias, et al. "Rescue of Mutant CFTR Trafficking Defect by the Investigational Compound MCG1516A." Cells vol. 11,1 (2022). doi: https://doi.org/10.3390/cells11010136
Lopes-Pacheco M, Bacalhau M, Ramalho SS, Silva IAL, Ferreira FC, Carlile GW, Thomas DY, Farinha CM, Hanrahan JW, Amaral MD. Rescue of Mutant CFTR Trafficking Defect by the Investigational Compound MCG1516A. Cells. 2022 Jan 01;11(1). doi: 10.3390/cells11010136. PMID: 35011698; PMCID: PMC8750248.
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Multimethod to prioritize projects evaluated in different formats.

MethodsX

Ramalho FD, Silva IS, Ekel PY, Martins CAPDS, Bernardes P, Libório MP.
PMID: 34430267
MethodsX. 2021 May 01;8:101371. doi: 10.1016/j.mex.2021.101371. eCollection 2021.

The prioritization of Research, Development & Innovation Projects is an essential step in the innovation management process. As a rule, it is carried out applying methods that allow one to process experts' preferences concerning each project according to established...

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Ramalho FD, Silva IS, Ekel PY, et al. Multimethod to prioritize projects evaluated in different formats. MethodsX. 2021;8:101371doi: 10.1016/j.mex.2021.101371.
Ramalho, F. D., Silva, I. S., Ekel, P. Y., Martins, C. A. P. D. S., Bernardes, P., & Libório, M. P. (2021). Multimethod to prioritize projects evaluated in different formats. MethodsX, 8101371. https://doi.org/10.1016/j.mex.2021.101371
Ramalho, Felipe Diniz, et al. "Multimethod to prioritize projects evaluated in different formats." MethodsX vol. 8 (2021): 101371. doi: https://doi.org/10.1016/j.mex.2021.101371
Ramalho FD, Silva IS, Ekel PY, Martins CAPDS, Bernardes P, Libório MP. Multimethod to prioritize projects evaluated in different formats. MethodsX. 2021 May 01;8:101371. doi: 10.1016/j.mex.2021.101371. eCollection 2021. PMID: 34430267; PMCID: PMC8374518.
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Dementia resulting from traumatic brain injury.

Dementia & neuropsychologia

Ramalho J, Castillo M.
PMID: 29213985
Dement Neuropsychol. 2015 Oct-Dec;9(4):356-368. doi: 10.1590/1980-57642015DN94000356.

Traumatic brain injury (TBI) represents a significant public health problem in modern societies. It is primarily a consequence of traffic-related accidents and falls. Other recently recognized causes include sports injuries and indirect forces such as shock waves from battlefield...

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Ramalho J, Castillo M. Dementia resulting from traumatic brain injury. Dement Neuropsychol. 2015;9(4):356-368doi: 10.1590/1980-57642015DN94000356.
Ramalho, J., & Castillo, M. (2015). Dementia resulting from traumatic brain injury. Dementia & neuropsychologia, 9(4), 356-368. https://doi.org/10.1590/1980-57642015DN94000356
Ramalho, Joana, and Castillo, Mauricio. "Dementia resulting from traumatic brain injury." Dementia & neuropsychologia vol. 9,4 (2015): 356-368. doi: https://doi.org/10.1590/1980-57642015DN94000356
Ramalho J, Castillo M. Dementia resulting from traumatic brain injury. Dement Neuropsychol. 2015 Oct-Dec;9(4):356-368. doi: 10.1590/1980-57642015DN94000356. PMID: 29213985; PMCID: PMC5619318.
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Application of Breast Ultrasound Elastography to Differentiate Intracapsular Collection from Silicone-Induced Granuloma of Breast Implant Capsule Complementarily to Contrast-Enhanced Breast Magnetic Resonance Imaging.

Breast cancer : basic and clinical research

de Faria Castro Fleury E, Gianini AC, Ayres V, Ramalho LC, Roveda D, de Oliveira VM.
PMID: 29147077
Breast Cancer (Auckl). 2017 Nov 02;11:1178223417737994. doi: 10.1177/1178223417737994. eCollection 2017.

BACKGROUND: To determine whether there is correlation between magnetic resonance imaging (MRI) findings and breast elastography to differentiate seroma/hematoma from silicone-induced granuloma of breast implant capsule (SIGBIC).METHODS: Prospective study of 99 patients with breast implants submitted to breast MRI...

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de Faria Castro Fleury E, Gianini AC, Ayres V, et al. Application of Breast Ultrasound Elastography to Differentiate Intracapsular Collection from Silicone-Induced Granuloma of Breast Implant Capsule Complementarily to Contrast-Enhanced Breast Magnetic Resonance Imaging. Breast Cancer (Auckl). 2017;11:1178223417737994doi: 10.1177/1178223417737994.
de Faria Castro Fleury, E., Gianini, A. C., Ayres, V., Ramalho, L. C., Roveda, D., & de Oliveira, V. M. (2017). Application of Breast Ultrasound Elastography to Differentiate Intracapsular Collection from Silicone-Induced Granuloma of Breast Implant Capsule Complementarily to Contrast-Enhanced Breast Magnetic Resonance Imaging. Breast cancer : basic and clinical research, 111178223417737994. https://doi.org/10.1177/1178223417737994
de Faria Castro Fleury, Eduardo, et al. "Application of Breast Ultrasound Elastography to Differentiate Intracapsular Collection from Silicone-Induced Granuloma of Breast Implant Capsule Complementarily to Contrast-Enhanced Breast Magnetic Resonance Imaging." Breast cancer : basic and clinical research vol. 11 (2017): 1178223417737994. doi: https://doi.org/10.1177/1178223417737994
de Faria Castro Fleury E, Gianini AC, Ayres V, Ramalho LC, Roveda D, de Oliveira VM. Application of Breast Ultrasound Elastography to Differentiate Intracapsular Collection from Silicone-Induced Granuloma of Breast Implant Capsule Complementarily to Contrast-Enhanced Breast Magnetic Resonance Imaging. Breast Cancer (Auckl). 2017 Nov 02;11:1178223417737994. doi: 10.1177/1178223417737994. eCollection 2017. PMID: 29147077; PMCID: PMC5673004.
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