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Showing 13 to 20 of 20 entries
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Optimization of Follicle Isolation for Bioengineering of Human Artificial Ovary.

Biopreservation and biobanking

Chen J, Isachenko E, Wang W, Du X, Wang M, Rahimi G, Mallmann P, Isachenko V.
PMID: 34936496
Biopreserv Biobank. 2021 Dec 20; doi: 10.1089/bio.2021.0060. Epub 2021 Dec 20.

No abstract available.

Unraveling Subcellular and Ultrastructural Changes During Vitrification of Human Spermatozoa: Effect of a Mitochondria-Targeted Antioxidant and a Permeable Cryoprotectant.

Frontiers in cell and developmental biology

Kumar P, Wang M, Isachenko E, Rahimi G, Mallmann P, Wang W, von Brandenstein M, Isachenko V.
PMID: 34277615
Front Cell Dev Biol. 2021 Jul 02;9:672862. doi: 10.3389/fcell.2021.672862. eCollection 2021.

Mitochondria-targeted antioxidants have great potential to counterbalance the generated reactive oxygen species (ROS) because they cross the inner membrane of the mitochondria. Still, their use was not reported in vitrified human spermatozoa. Our laboratory has successfully vitrified spermatozoa without...

Ultra-rapid freezing and storage of rat embryos in an electric refrigerator at -130 degree C without liquid cryo-agents, with ultra-short exposure in the freezing medium and direct rehydration after thawing.

Cryo letters

Isachenko VV, Alabart JL, Isachenko EF, Bezugly ND, Michelmann HW.
PMID: 12148059
Cryo Letters. 2000 Jan-Feb;21(1):13-18.

An ultra-rapid freezing technique for embryos has been developed. This procedure involves: ultra-short exposure to cryoprotectants; freezing of embryos in a metallic powder pre-cooled in an electric refrigerator at -130 degree C; storage of embryos ina refrigerator at -130...

Improvement of attachment rate of bovine embryos after transfer with trophoblastic fragments.

Theriogenology

Isachenko VV, Soler C, Ostashko FI, Isachenko EF.
PMID: 16727541
Theriogenology. 1994 Aug 01;42(2):339-44. doi: 10.1016/0093-691x(94)90278-x.

Fragments of trophoblastic vesicles have been shown to improve embryo development when used in co-culture. We wished to investigate the effect of trophoblastic fragments on establishment of pregnancy after embryo transfer. Embryos (n=129) frozen by direct immersion in liquid...

Spectral studies of niflumic acid aggregation in dissolved, solid and adsorbed states.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

Baranov DA, Vysotskaya SO, Zarochentseva EP, Lisachenko DA, Nosova DA, Tsyganenko AA.
PMID: 31176155
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Nov 05;222:117224. doi: 10.1016/j.saa.2019.117224. Epub 2019 May 30.

FTIR spectra of niflumic acid in solid state, dissolved in CCl

Correction: Long-Time Cooling before Cryopreservation Decreased Translocation of Phosphatidylserine (Ptd-L-Ser) in Human Ovarian Tissue.

PloS one

Isachenko V, Todorov P, Isachenko E, Rahimi G, Tchorbanov A, Mihaylova N, Manoylov I, Mallmann P, Merzenich M.
PMID: 30794685
PLoS One. 2019 Feb 22;14(2):e0212961. doi: 10.1371/journal.pone.0212961. eCollection 2019.

[This corrects the article DOI: 10.1371/journal.pone.0129108.].

Cryo-banking of human spermatozoa by aseptic cryoprotectants-free vitrification in liquid air: Positive effect of elevated warming temperature.

Cell and tissue banking

Diaz-Jimenez M, Wang M, Wang W, Isachenko E, Rahimi G, Kumar P, Mallmann P, von Brandenstein M, Hidalgo M, Isachenko V.
PMID: 33608835
Cell Tissue Bank. 2021 Feb 19; doi: 10.1007/s10561-021-09904-0. Epub 2021 Feb 19.

Cryoprotectant-free vitrification is a common method for spermatozoa cryopreservation by direct plunging into liquid nitrogen. However, the commercial liquid nitrogen could be potentially contaminated by microorganisms. Warming temperature plays an essential role for quality of human spermatozoa after vitrification....

High cryo-resistance of SARS-CoV-2 virus: Increased risk of re-contamination at transplantation of cryopreserved ovarian tissue after COVID-19 pandemic.

Cryobiology

Isachenko V, Isachenko E, Mallmann P, Rahimi G.
PMID: 34571024
Cryobiology. 2021 Sep 25; doi: 10.1016/j.cryobiol.2021.09.009. Epub 2021 Sep 25.

Cryopreservation and re-transplantation of ovarian tissue after anticancer treatment is important medical technology. Today, during a pandemic, the risk of contamination of transplanted cells with SARS-CoV-2 virus is extremely high. Data about cryo-resistance (virulence and/or infectivity) of SARS-CoV-2 are...

Showing 13 to 20 of 20 entries