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Genome-wide association study of INDELs identified four novel susceptibility loci associated with lung cancer risk.

International journal of cancer

Dai J, Huang M, Amos CI, Hung RJ, Tardon A, Andrew A, Chen C, Christiani DC, Albanes D, Rennert G, Fan J, Goodman G, Liu G, Field JK, Grankvist K, Kiemeney LA, Le Marchand L, Schabath MB, Johansson M, Aldrich MC, Johansson M, Caporaso N, Lazarus P, Lam S, Bojesen SE, Arnold S, Landi MT, Risch A, Wichmann HE, Bickeboller H, Brennan P, Shete S, Melander O, Brunnstrom H, Zienolddiny S, Woll P, Stevens V, Hu Z, Shen H.
PMID: 31577861
Int J Cancer. 2020 May 15;146(10):2855-2864. doi: 10.1002/ijc.32698. Epub 2019 Oct 31.

Genome-wide association studies (GWAS) have identified 45 susceptibility loci associated with lung cancer. Only less than SNPs, small insertions and deletions (INDELs) are the second most abundant genetic polymorphisms in the human genome. INDELs are highly associated with multiple...

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Dai J, Huang M, Amos CI, et al. Genome-wide association study of INDELs identified four novel susceptibility loci associated with lung cancer risk. Int J Cancer. 2019;146(10):2855-2864doi: 10.1002/ijc.32698.
Dai, J., Huang, M., Amos, C. I., Hung, R. J., Tardon, A., Andrew, A., Chen, C., Christiani, D. C., Albanes, D., Rennert, G., Fan, J., Goodman, G., Liu, G., Field, J. K., Grankvist, K., Kiemeney, L. A., Le Marchand, L., Schabath, M. B., Johansson, M., Aldrich, M. C., Johansson, M., Caporaso, N., Lazarus, P., Lam, S., Bojesen, S. E., Arnold, S., Landi, M. T., Risch, A., Wichmann, H. E., Bickeboller, H., Brennan, P., Shete, S., Melander, O., Brunnstrom, H., Zienolddiny, S., Woll, P., Stevens, V., Hu, Z., & Shen, H. (2020). Genome-wide association study of INDELs identified four novel susceptibility loci associated with lung cancer risk. International journal of cancer, 146(10), 2855-2864. https://doi.org/10.1002/ijc.32698
Dai, Juncheng, et al. "Genome-wide association study of INDELs identified four novel susceptibility loci associated with lung cancer risk." International journal of cancer vol. 146,10 (2020): 2855-2864. doi: https://doi.org/10.1002/ijc.32698
Dai J, Huang M, Amos CI, Hung RJ, Tardon A, Andrew A, Chen C, Christiani DC, Albanes D, Rennert G, Fan J, Goodman G, Liu G, Field JK, Grankvist K, Kiemeney LA, Le Marchand L, Schabath MB, Johansson M, Aldrich MC, Johansson M, Caporaso N, Lazarus P, Lam S, Bojesen SE, Arnold S, Landi MT, Risch A, Wichmann HE, Bickeboller H, Brennan P, Shete S, Melander O, Brunnstrom H, Zienolddiny S, Woll P, Stevens V, Hu Z, Shen H. Genome-wide association study of INDELs identified four novel susceptibility loci associated with lung cancer risk. Int J Cancer. 2020 May 15;146(10):2855-2864. doi: 10.1002/ijc.32698. Epub 2019 Oct 31. PMID: 31577861; PMCID: PMC7101262.
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A placebo-controlled randomized study with testosterone in Klinefelter syndrome: beneficial effects on body composition.

Endocrine connections

Høst C, Bojesen A, Erlandsen M, Groth KA, Kristensen K, Jurik AG, Birkebæk NH, Gravholt CH.
PMID: 31394497
Endocr Connect. 2019 Sep;8(9):1250-1261. doi: 10.1530/EC-19-0323.

CONTEXT AND OBJECTIVE: Males with Klinefelter syndrome (KS) are typically hypogonadal with a high incidence of metabolic disease, increased body fat and mortality. Testosterone treatment of hypogonadal patients decrease fat mass, increase lean body mass and improve insulin sensitivity,...

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Høst C, Bojesen A, Erlandsen M, et al. A placebo-controlled randomized study with testosterone in Klinefelter syndrome: beneficial effects on body composition. Endocr Connect. 2019;8(9):1250-1261doi: 10.1530/EC-19-0323.
Høst, C., Bojesen, A., Erlandsen, M., Groth, K. A., Kristensen, K., Jurik, A. G., Birkebæk, N. H., & Gravholt, C. H. (2019). A placebo-controlled randomized study with testosterone in Klinefelter syndrome: beneficial effects on body composition. Endocrine connections, 8(9), 1250-1261. https://doi.org/10.1530/EC-19-0323
Høst, Christian, et al. "A placebo-controlled randomized study with testosterone in Klinefelter syndrome: beneficial effects on body composition." Endocrine connections vol. 8,9 (2019): 1250-1261. doi: https://doi.org/10.1530/EC-19-0323
Høst C, Bojesen A, Erlandsen M, Groth KA, Kristensen K, Jurik AG, Birkebæk NH, Gravholt CH. A placebo-controlled randomized study with testosterone in Klinefelter syndrome: beneficial effects on body composition. Endocr Connect. 2019 Sep;8(9):1250-1261. doi: 10.1530/EC-19-0323. PMID: 31394497; PMCID: PMC6733367.
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Differential effects of age at illness onset on verbal memory functions in antipsychotic-naïve schizophrenia patients aged 12-43 years.

Psychological medicine

Fagerlund B, Pantelis C, Jepsen JRM, Raghava JM, Rostrup E, Thomas MB, Nielsen MØ, Bojesen K, Jensen KG, Stentebjerg-Decara M, Klauber DG, Rudå D, Ebdrup BH, Jessen K, Sigvard A, Tangmose K, Jeppesen P, Correll CU, Fink-Jensen A, Pagsberg AK, Glenthøj BY.
PMID: 32156323
Psychol Med. 2021 Jul;51(9):1570-1580. doi: 10.1017/S0033291720000409. Epub 2020 Mar 11.

BACKGROUND: The typical onset of schizophrenia coincides with the maturational peak in cognition; however, for a significant proportion of patients the onset is before age 18 and after age 30 years. While cognitive deficits are considered core features of...

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Fagerlund B, Pantelis C, Jepsen JRM, et al. Differential effects of age at illness onset on verbal memory functions in antipsychotic-naïve schizophrenia patients aged 12-43 years. Psychol Med. 2020;51(9):1570-1580doi: 10.1017/S0033291720000409.
Fagerlund, B., Pantelis, C., Jepsen, J. R. M., Raghava, J. M., Rostrup, E., Thomas, M. B., Nielsen, M. �. �., Bojesen, K., Jensen, K. G., Stentebjerg-Decara, M., Klauber, D. G., Rudå, D., Ebdrup, B. H., Jessen, K., Sigvard, A., Tangmose, K., Jeppesen, P., Correll, C. U., Fink-Jensen, A., Pagsberg, A. K., & Glenthøj, B. Y. (2021). Differential effects of age at illness onset on verbal memory functions in antipsychotic-naïve schizophrenia patients aged 12-43 years. Psychological medicine, 51(9), 1570-1580. https://doi.org/10.1017/S0033291720000409
Fagerlund, Birgitte, et al. "Differential effects of age at illness onset on verbal memory functions in antipsychotic-naïve schizophrenia patients aged 12-43 years." Psychological medicine vol. 51,9 (2021): 1570-1580. doi: https://doi.org/10.1017/S0033291720000409
Fagerlund B, Pantelis C, Jepsen JRM, Raghava JM, Rostrup E, Thomas MB, Nielsen MØ, Bojesen K, Jensen KG, Stentebjerg-Decara M, Klauber DG, Rudå D, Ebdrup BH, Jessen K, Sigvard A, Tangmose K, Jeppesen P, Correll CU, Fink-Jensen A, Pagsberg AK, Glenthøj BY. Differential effects of age at illness onset on verbal memory functions in antipsychotic-naïve schizophrenia patients aged 12-43 years. Psychol Med. 2021 Jul;51(9):1570-1580. doi: 10.1017/S0033291720000409. Epub 2020 Mar 11. PMID: 32156323.
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High-resolution structure of native toxin A from Clostridioides difficile.

EMBO reports

Aminzadeh A, Larsen CE, Boesen T, Jørgensen R.
PMID: 34817920
EMBO Rep. 2022 Jan 05;23(1):e53597. doi: 10.15252/embr.202153597. Epub 2021 Nov 24.

Clostridioides difficile infections have emerged as the leading cause of healthcare-associated infectious diarrhea. Disease symptoms are mainly caused by the virulence factors, TcdA and TcdB, which are large homologous multidomain proteins. Here, we report a 2.8 Å resolution cryo-EM...

Cite
Aminzadeh A, Larsen CE, Boesen T, et al. High-resolution structure of native toxin A from Clostridioides difficile. EMBO Rep. 2021;23(1):e53597doi: 10.15252/embr.202153597.
Aminzadeh, A., Larsen, C. E., Boesen, T., & Jørgensen, R. (2022). High-resolution structure of native toxin A from Clostridioides difficile. EMBO reports, 23(1), e53597. https://doi.org/10.15252/embr.202153597
Aminzadeh, Aria, et al. "High-resolution structure of native toxin A from Clostridioides difficile." EMBO reports vol. 23,1 (2022): e53597. doi: https://doi.org/10.15252/embr.202153597
Aminzadeh A, Larsen CE, Boesen T, Jørgensen R. High-resolution structure of native toxin A from Clostridioides difficile. EMBO Rep. 2022 Jan 05;23(1):e53597. doi: 10.15252/embr.202153597. Epub 2021 Nov 24. PMID: 34817920.
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Doppler ultrasound predicts successful discontinuation of biological DMARDs in rheumatoid arthritis patients in clinical remission.

Rheumatology (Oxford, England)

Terslev L, Brahe CH, Hetland ML, Georgiadis S, Ellegaard K, Juul L, Huynh T, Døhn UM, Fana V, Møller T, Krabbe S, Ørnbjerg LM, Glinatsi D, Røgind H, Hansen A, Nørregaard J, Jacobsen S, Jensen DV, Manilo N, Asmussen K, Boesen M, Rastiemadabadi Z, Morsel-Carlsen L, Møller JM, Krogh NS, Østergaard M.
PMID: 33748831
Rheumatology (Oxford). 2021 Dec 01;60(12):5549-5559. doi: 10.1093/rheumatology/keab276.

OBJECTIVE: To assess the ability of ultrasound to predict successful tapering and successful discontinuation of biological DMARDs (bDMARDs) at the 2-year follow-up in RA patients in sustained remission.METHODS: Patients in sustained remission (DAS28-CRP ≤ 2.6) and with no radiographic...

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Terslev L, Brahe CH, Hetland ML, et al. Doppler ultrasound predicts successful discontinuation of biological DMARDs in rheumatoid arthritis patients in clinical remission. Rheumatology (Oxford). 2021;60(12):5549-5559doi: 10.1093/rheumatology/keab276.
Terslev, L., Brahe, C. H., Hetland, M. L., Georgiadis, S., Ellegaard, K., Juul, L., Huynh, T., Døhn, U. M., Fana, V., Møller, T., Krabbe, S., Ørnbjerg, L. M., Glinatsi, D., Røgind, H., Hansen, A., Nørregaard, J., Jacobsen, S., Jensen, D. V., Manilo, N., Asmussen, K., Boesen, M., Rastiemadabadi, Z., Morsel-Carlsen, L., Møller, J. M., Krogh, N. S., & Østergaard, M. (2021). Doppler ultrasound predicts successful discontinuation of biological DMARDs in rheumatoid arthritis patients in clinical remission. Rheumatology (Oxford, England), 60(12), 5549-5559. https://doi.org/10.1093/rheumatology/keab276
Terslev, Lene, et al. "Doppler ultrasound predicts successful discontinuation of biological DMARDs in rheumatoid arthritis patients in clinical remission." Rheumatology (Oxford, England) vol. 60,12 (2021): 5549-5559. doi: https://doi.org/10.1093/rheumatology/keab276
Terslev L, Brahe CH, Hetland ML, Georgiadis S, Ellegaard K, Juul L, Huynh T, Døhn UM, Fana V, Møller T, Krabbe S, Ørnbjerg LM, Glinatsi D, Røgind H, Hansen A, Nørregaard J, Jacobsen S, Jensen DV, Manilo N, Asmussen K, Boesen M, Rastiemadabadi Z, Morsel-Carlsen L, Møller JM, Krogh NS, Østergaard M. Doppler ultrasound predicts successful discontinuation of biological DMARDs in rheumatoid arthritis patients in clinical remission. Rheumatology (Oxford). 2021 Dec 01;60(12):5549-5559. doi: 10.1093/rheumatology/keab276. PMID: 33748831.
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The US Food and Drug Administration's authorisation of Purdue's controlled-release methylphenidate for adult ADHD: comments on the regulatory practice.

Journal of the Royal Society of Medicine

Boesen K, Juhl Jørgensen K, Gøtzsche PC.
PMID: 33759627
J R Soc Med. 2021 Aug;114(8):377-380. doi: 10.1177/0141076821994535. Epub 2021 Mar 24.

No abstract available.

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Boesen K, Juhl Jørgensen K, Gøtzsche PC. The US Food and Drug Administration's authorisation of Purdue's controlled-release methylphenidate for adult ADHD: comments on the regulatory practice. J R Soc Med. 2021;114(8):377-380doi: 10.1177/0141076821994535.
Boesen, K., Juhl Jørgensen, K., & Gøtzsche, P. C. (2021). The US Food and Drug Administration's authorisation of Purdue's controlled-release methylphenidate for adult ADHD: comments on the regulatory practice. Journal of the Royal Society of Medicine, 114(8), 377-380. https://doi.org/10.1177/0141076821994535
Boesen, Kim, et al. "The US Food and Drug Administration's authorisation of Purdue's controlled-release methylphenidate for adult ADHD: comments on the regulatory practice." Journal of the Royal Society of Medicine vol. 114,8 (2021): 377-380. doi: https://doi.org/10.1177/0141076821994535
Boesen K, Juhl Jørgensen K, Gøtzsche PC. The US Food and Drug Administration's authorisation of Purdue's controlled-release methylphenidate for adult ADHD: comments on the regulatory practice. J R Soc Med. 2021 Aug;114(8):377-380. doi: 10.1177/0141076821994535. Epub 2021 Mar 24. PMID: 33759627; PMCID: PMC8361378.
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CYP2C9 and 3A4 play opposing roles in bioactivation and detoxification of diphenylamine NSAIDs.

Biochemical pharmacology

Schleiff MA, Crosby S, Blue M, Schleiff BM, Boysen G, Miller GP.
PMID: 34748821
Biochem Pharmacol. 2021 Dec;194:114824. doi: 10.1016/j.bcp.2021.114824. Epub 2021 Nov 05.

Diphenylamine NSAIDs are taken frequently for chronic pain conditions, yet their use may potentiate hepatotoxicity risks through poorly characterized metabolic mechanisms. Our previous work revealed that seven marketed or withdrawn diphenylamine NSAIDs undergo bioactivation into quinone-species metabolites, whose reaction...

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Schleiff MA, Crosby S, Blue M, et al. CYP2C9 and 3A4 play opposing roles in bioactivation and detoxification of diphenylamine NSAIDs. Biochem Pharmacol. 2021;194:114824doi: 10.1016/j.bcp.2021.114824.
Schleiff, M. A., Crosby, S., Blue, M., Schleiff, B. M., Boysen, G., & Miller, G. P. (2021). CYP2C9 and 3A4 play opposing roles in bioactivation and detoxification of diphenylamine NSAIDs. Biochemical pharmacology, 194114824. https://doi.org/10.1016/j.bcp.2021.114824
Schleiff, Mary Alexandra, et al. "CYP2C9 and 3A4 play opposing roles in bioactivation and detoxification of diphenylamine NSAIDs." Biochemical pharmacology vol. 194 (2021): 114824. doi: https://doi.org/10.1016/j.bcp.2021.114824
Schleiff MA, Crosby S, Blue M, Schleiff BM, Boysen G, Miller GP. CYP2C9 and 3A4 play opposing roles in bioactivation and detoxification of diphenylamine NSAIDs. Biochem Pharmacol. 2021 Dec;194:114824. doi: 10.1016/j.bcp.2021.114824. Epub 2021 Nov 05. PMID: 34748821.
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Correction to: Cross-sectional study of medical advertisements in a national general medical journal: evidence, cost, and safe use of advertised versus comparative drugs.

Research integrity and peer review

Boesen K, Simonsen AL, Jørgensen KJ, Gøtzsche PC.
PMID: 34116717
Res Integr Peer Rev. 2021 Jun 11;6(1):10. doi: 10.1186/s41073-021-00114-6.

No abstract available.

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Boesen K, Simonsen AL, Jørgensen KJ, et al. Correction to: Cross-sectional study of medical advertisements in a national general medical journal: evidence, cost, and safe use of advertised versus comparative drugs. Res Integr Peer Rev. 2021;6(1):10doi: 10.1186/s41073-021-00114-6.
Boesen, K., Simonsen, A. L., Jørgensen, K. J., & Gøtzsche, P. C. (2021). Correction to: Cross-sectional study of medical advertisements in a national general medical journal: evidence, cost, and safe use of advertised versus comparative drugs. Research integrity and peer review, 6(1), 10. https://doi.org/10.1186/s41073-021-00114-6
Boesen, Kim, et al. "Correction to: Cross-sectional study of medical advertisements in a national general medical journal: evidence, cost, and safe use of advertised versus comparative drugs." Research integrity and peer review vol. 6,1 (2021): 10. doi: https://doi.org/10.1186/s41073-021-00114-6
Boesen K, Simonsen AL, Jørgensen KJ, Gøtzsche PC. Correction to: Cross-sectional study of medical advertisements in a national general medical journal: evidence, cost, and safe use of advertised versus comparative drugs. Res Integr Peer Rev. 2021 Jun 11;6(1):10. doi: 10.1186/s41073-021-00114-6. PMID: 34116717; PMCID: PMC8196460.
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Behavioural changes in an adopted teenager.

Practical neurology

Soni A, Booysen G, Heckmann JM.
PMID: 34824155
Pract Neurol. 2021 Nov 25; doi: 10.1136/practneurol-2021-003236. Epub 2021 Nov 25.

No abstract available.

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Soni A, Booysen G, Heckmann JM. Behavioural changes in an adopted teenager. Pract Neurol. 2021;doi: 10.1136/practneurol-2021-003236.
Soni, A., Booysen, G., & Heckmann, J. M. (2021). Behavioural changes in an adopted teenager. Practical neurology, . https://doi.org/10.1136/practneurol-2021-003236
Soni, Aayesha, et al. "Behavioural changes in an adopted teenager." Practical neurology vol. (2021). doi: https://doi.org/10.1136/practneurol-2021-003236
Soni A, Booysen G, Heckmann JM. Behavioural changes in an adopted teenager. Pract Neurol. 2021 Nov 25; doi: 10.1136/practneurol-2021-003236. Epub 2021 Nov 25. PMID: 34824155.
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DEB-FAPy-dG Adducts of 1,3-Butadiene: Synthesis, Structural Characterization, and Formation in 1,2,3,4-Diepoxybutane Treated DNA*.

Chemistry (Weinheim an der Bergstrasse, Germany)

Pujari SS, Jokipii Krueger CC, Chao C, Hutchins S, Hurben AK, Boysen G, Tretyakova N.
PMID: 34767297
Chemistry. 2021 Nov 12; doi: 10.1002/chem.202103245. Epub 2021 Nov 12.

Metabolic activation of the human carcinogen 1,3-butadiene (BD) by cytochrome 450 monooxygenases gives rise to a genotoxic diepoxide, 1,2,3,4-diepoxybutane (DEB). This reactive electrophile alkylates guanine bases in DNA to produce N7-(2-hydroxy-3,4-epoxy-1-yl)-dG (N7-DE-dG) adducts. Because of the positive charge at...

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Pujari SS, Jokipii Krueger CC, Chao C, et al. DEB-FAPy-dG Adducts of 1,3-Butadiene: Synthesis, Structural Characterization, and Formation in 1,2,3,4-Diepoxybutane Treated DNA*. Chemistry. 2021;doi: 10.1002/chem.202103245.
Pujari, S. S., Jokipii Krueger, C. C., Chao, C., Hutchins, S., Hurben, A. K., Boysen, G., & Tretyakova, N. (2021). DEB-FAPy-dG Adducts of 1,3-Butadiene: Synthesis, Structural Characterization, and Formation in 1,2,3,4-Diepoxybutane Treated DNA*. Chemistry (Weinheim an der Bergstrasse, Germany), . https://doi.org/10.1002/chem.202103245
Pujari, Suresh S, et al. "DEB-FAPy-dG Adducts of 1,3-Butadiene: Synthesis, Structural Characterization, and Formation in 1,2,3,4-Diepoxybutane Treated DNA*." Chemistry (Weinheim an der Bergstrasse, Germany) vol. (2021). doi: https://doi.org/10.1002/chem.202103245
Pujari SS, Jokipii Krueger CC, Chao C, Hutchins S, Hurben AK, Boysen G, Tretyakova N. DEB-FAPy-dG Adducts of 1,3-Butadiene: Synthesis, Structural Characterization, and Formation in 1,2,3,4-Diepoxybutane Treated DNA*. Chemistry. 2021 Nov 12; doi: 10.1002/chem.202103245. Epub 2021 Nov 12. PMID: 34767297.
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High-resolution structure of native toxin A from Clostridioides difficile.

EMBO reports

Aminzadeh A, Larsen CE, Boesen T, Jørgensen R.
PMID: 34817920
EMBO Rep. 2021 Nov 24;e53597. doi: 10.15252/embr.202153597. Epub 2021 Nov 24.

Clostridioides difficile infections have emerged as the leading cause of healthcare-associated infectious diarrhea. Disease symptoms are mainly caused by the virulence factors, TcdA and TcdB, which are large homologous multidomain proteins. Here, we report a 2.8 Å resolution cryo-EM...

Cite
Aminzadeh A, Larsen CE, Boesen T, et al. High-resolution structure of native toxin A from Clostridioides difficile. EMBO Rep. 2021;e53597doi: 10.15252/embr.202153597.
Aminzadeh, A., Larsen, C. E., Boesen, T., & Jørgensen, R. (2021). High-resolution structure of native toxin A from Clostridioides difficile. EMBO reports, e53597. https://doi.org/10.15252/embr.202153597
Aminzadeh, Aria, et al. "High-resolution structure of native toxin A from Clostridioides difficile." EMBO reports vol. (2021): e53597. doi: https://doi.org/10.15252/embr.202153597
Aminzadeh A, Larsen CE, Boesen T, Jørgensen R. High-resolution structure of native toxin A from Clostridioides difficile. EMBO Rep. 2021 Nov 24;e53597. doi: 10.15252/embr.202153597. Epub 2021 Nov 24. PMID: 34817920.
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Bioactivation of Isoxazole-Containing Bromodomain and Extra-Terminal Domain (BET) Inhibitors.

Metabolites

Flynn NR, Ward MD, Schleiff MA, Laurin CMC, Farmer R, Conway SJ, Boysen G, Swamidass SJ, Miller GP.
PMID: 34203690
Metabolites. 2021 Jun 15;11(6). doi: 10.3390/metabo11060390.

The 3,5-dimethylisoxazole motif has become a useful and popular acetyl-lysine mimic employed in isoxazole-containing bromodomain and extra-terminal (BET) inhibitors but may introduce the potential for bioactivations into toxic reactive metabolites. As a test, we coupled deep neural models for...

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Flynn NR, Ward MD, Schleiff MA, et al. Bioactivation of Isoxazole-Containing Bromodomain and Extra-Terminal Domain (BET) Inhibitors. Metabolites. 2021;11(6)doi: 10.3390/metabo11060390.
Flynn, N. R., Ward, M. D., Schleiff, M. A., Laurin, C. M. C., Farmer, R., Conway, S. J., Boysen, G., Swamidass, S. J., & Miller, G. P. (2021). Bioactivation of Isoxazole-Containing Bromodomain and Extra-Terminal Domain (BET) Inhibitors. Metabolites, 11(6), . https://doi.org/10.3390/metabo11060390
Flynn, Noah R, et al. "Bioactivation of Isoxazole-Containing Bromodomain and Extra-Terminal Domain (BET) Inhibitors." Metabolites vol. 11,6 (2021). doi: https://doi.org/10.3390/metabo11060390
Flynn NR, Ward MD, Schleiff MA, Laurin CMC, Farmer R, Conway SJ, Boysen G, Swamidass SJ, Miller GP. Bioactivation of Isoxazole-Containing Bromodomain and Extra-Terminal Domain (BET) Inhibitors. Metabolites. 2021 Jun 15;11(6). doi: 10.3390/metabo11060390. PMID: 34203690; PMCID: PMC8232216.
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