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Showing 13 to 24 of 223 entries
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T-cell responses against meningococcal antigens.

Methods in molecular medicine

Næss LM, Oftung F, Aase A, Michaelsen TE, Pollard AJ.
PMID: 21336766
Methods Mol Med. 2001;66:339-48. doi: 10.1385/1-59259-148-5:339.

T-cells recognize protein antigens as short peptide fragments (8-20 amino acids) bound to major histocompatibility complex (MHC) molecules on the surface of antigen-presenting cells (APCs). A prerequisite for antigen-specific T-cell activation is antigen uptake, enzymatic degradation, and recycling of...

Amyloid-β peptide is a substrate of the human 20S proteasome.

ACS chemical neuroscience

Zhao X, Yang J.
PMID: 21116456
ACS Chem Neurosci. 2010 Oct 20;1(10):655-660. doi: 10.1021/cn100067e.

Intraneuronal accumulation of ubiquitin conjugates is a pathological feature of neurodegenerative disorders such as Alzheimer's disease (AD). Previous reports propose that accumulation of ubiquitinated species in AD is a result of inhibition of proteasomal activity by amyloid-β (Aβ) peptides,...

SDS-PAGE Peptide-Mapping Procedure (Cleveland Method).

CSH protocols

Simpson RJ.
PMID: 21357014
CSH Protoc. 2007 Feb 01;2007:pdb.prot4591. doi: 10.1101/pdb.prot4591.

INTRODUCTIONThe Cleveland method for peptide mapping of proteins relies on partial hydrolysis of the protein yielding a considerable number of large peptides that can be readily identified by SDS-PAGE or tricine-SDS-PAGE. Small peptides obtained with a total digest are...

A Self-Replicating Peptide under Ionic Control.

Angewandte Chemie (International ed. in English)

Yao S, Ghosh I, Zutshi R, Chmielewski J.
PMID: 29711158
Angew Chem Int Ed Engl. 1998 Mar 02;37(4):478-481. doi: 10.1002/(SICI)1521-3773(19980302)37:4<478::AID-ANIE478>3.0.CO;2-V.

The chemical coupling of two peptide fragments to give the peptide K1 K2 (shown in the helical wheel diagram on the right) is autocatalytic at high NaClO

A prospective study on peptide mapping of human fatigue saliva markers based on magnetic beads.

Experimental and therapeutic medicine

Xu Y, Xiao D, Zhang H, He L, Gu Y, Peng X, Gao X, Liu Z, Zhang J.
PMID: 30936969
Exp Ther Med. 2019 Apr;17(4):2995-3002. doi: 10.3892/etm.2019.7293. Epub 2019 Feb 22.

In order to explore convenient and stable fatigue markers, we studied various high-molecular-weight peptide fragments under fatigue state and non-fatigue state in the saliva using time of flight mass spectrometry. The saliva samples were collected from 10 healthy volunteers...

Formation and morphogenesis of a cuttlebone's aragonite biomineral structures for the common cuttlefish (Sepia officinalis) on the nanoscale: Revisited.

Journal of colloid and interface science

Čadež V, Škapin SD, Leonardi A, Križaj I, Kazazić S, Salopek-Sondi B, Sondi I.
PMID: 28822865
J Colloid Interface Sci. 2017 Dec 15;508:95-104. doi: 10.1016/j.jcis.2017.08.028. Epub 2017 Aug 10.

This study describes and examines the structural and morphological properties of the hierarchically organized, aragonite cuttlebone forms for the common cuttlefish (Sepia officinalis, L.), including its main structural parts, the dorsal shield, and the chambers. Specifically, it complements the...

Natural Occurrence of Chilli veinal mottle virus on Capsicum chinense in China.

Plant disease

Wang J, Liu Z, Niu S, Peng M, Wang D, Weng Z, Xiong Z.
PMID: 30786572
Plant Dis. 2006 Mar;90(3):377. doi: 10.1094/PD-90-0377C.

An outbreak of a viral disease on chili pepper (Capsicum chinense Jacp. cv. Yellow Lantern) occurred in Hainan Province, China during 2003 and 2004. The disease was prevalent in five chili-producing counties surveyed. Leaves of infected plants initially displayed...

The Metalloprotease Meprin β Is an Alternative β-Secretase of APP.

Frontiers in molecular neuroscience

Becker-Pauly C, Pietrzik CU.
PMID: 28105004
Front Mol Neurosci. 2017 Jan 05;9:159. doi: 10.3389/fnmol.2016.00159. eCollection 2016.

The membrane bound metalloprotease meprin β is important for collagen fibril assembly in connective tissue formation and for the detachment of the intestinal mucus layer for proper barrier function. Recent proteomic studies revealed dozens of putative new substrates of...

Ultrasensitive tumour-penetrating nanosensors of protease activity.

Nature biomedical engineering

Kwon EJ, Dudani JS, Bhatia SN.
PMID: 28970963
Nat Biomed Eng. 2017;1. doi: 10.1038/s41551-017-0054. Epub 2017 Apr 10.

The ability to identify cancer lesions with endogenous biomarkers is currently limited to tumours ~1 cm in diameter. We recently reported an exogenously administered tumour-penetrating nanosensor that sheds, in response to tumour-specific proteases, peptide fragments that can then be...

Effect of osmolytes on the conformation and aggregation of some amyloid peptides: CD spectroscopic data.

Data in brief

Inayathullah M, Rajadas J.
PMID: 27222868
Data Brief. 2016 May 04;7:1643-51. doi: 10.1016/j.dib.2016.04.070. eCollection 2016 Jun.

Protein misfolding and aggregation are responsible for a large number of diseases called protein conformational diseases or disorders that include Alzheimer׳s disease, Huntington׳s diseases, Prion related encephalopathies and type-II diabetes (http://dx.doi.org/10.1038/35041139) (Kopito and Ron, 2000) [1]. A variety of...

Identification of combined T-cell and B-cell reactive .

Annals of translational medicine

Wang L, Gao J, Lan X, Zhao H, Shang X, Tian F, Wen H, Ding J, Luo L, Ma X.
PMID: 31930053
Ann Transl Med. 2019 Nov;7(22):652. doi: 10.21037/atm.2019.10.87.

BACKGROUND: Identification of combined T-cell and B-cell reactive Eg95 antigens for the potential development of a multi-epitope vaccine against METHODS: This study involved the recombinant expression of Eg95 along with associated immune rabbit antiserum preparation. Bioinformatics technology was used...

The Role of Multiscale Protein Dynamics in Antigen Presentation and T Lymphocyte Recognition.

Frontiers in immunology

Eccleston RC, Wan S, Dalchau N, Coveney PV.
PMID: 28740497
Front Immunol. 2017 Jul 10;8:797. doi: 10.3389/fimmu.2017.00797. eCollection 2017.

T lymphocytes are stimulated when they recognize short peptides bound to class I proteins of the major histocompatibility complex (MHC) protein, as peptide-MHC complexes. Due to the diversity in T-cell receptor (TCR) molecules together with both the peptides and...

Showing 13 to 24 of 223 entries