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Showing 25 to 36 of 241 entries
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Improved probe selection for DNA arrays using nonparametric kernel density estimation.

Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference

Fu Q, Borneman J, Ye J, Chrobak M.
PMID: 17282330
Conf Proc IEEE Eng Med Biol Soc. 2005;2006:902-5. doi: 10.1109/IEMBS.2005.1616561.

Oligonucleotide fingerprinting of rRNA genes (OFRG) is a method for identifying arrayed ribosomal RNA genes (rDNA) through a series of hybridization experiments with short oligonucleotide probes. Due to its low cost and high speed, it is an effective tool...

HLA Genotyping and Antibody Characterization Using the Luminexâ„¢ Multiplex Technology.

Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie

Heinemann FM.
PMID: 21049078
Transfus Med Hemother. 2009;36(4):273-278. doi: 10.1159/000228834. Epub 2009 Jul 21.

The Luminex-based human leukocyte antigen (HLA) antibody screening technology is widespread used in laboratories affiliated to kidney transplantation programs and enables both screening (i.e. the definition of positive or negative antibody status) and antibody identification with high sensitivity and...

Synthetic oligonucleotide probes for detection of mercury-resistance genes in environmental freshwater microbial communities in response to pollutants.

World journal of microbiology & biotechnology

Mirgain I, Hagnere C, Green GA, Harf C, Monteil H.
PMID: 24425330
World J Microbiol Biotechnol. 1992 Jan;8(1):30-8. doi: 10.1007/BF01200680.

Mercury-resistance genes were detected byin situ hybridization using new synthetic oligonucleotide probes specific formerA andmerB genes according to the published sequences of the corresponding enzymes. These DNA probes were used for the detection of specific mercury-resistant microorganisms isolated from...

Association of polymorphisms of THBS2 and HSPA8 with hypertension in Japanese individuals with chronic kidney disease.

Molecular medicine reports

Oguri M, Kato K, Yokoi K, Watanabe S, Metoki N, Yoshida H, Satoh K, Aoyagi Y, Nishigaki Y, Yoshida H, Nozawa Y, Yamada Y.
PMID: 21475814
Mol Med Rep. 2009 Mar-Apr;2(2):205-11. doi: 10.3892/mmr_00000085.

The purpose of the present study was to identify gene polymorphisms that confer susceptibility to hypertension in individuals with chronic kidney disease (CKD), thereby contributing to the prediction of genetic risk for this condition. The study population comprised 1824...

Hybridization of oligonucleotide probes in aqueous solutions: washing in buffers containing quaternary ammonium salts.

CSH protocols

Sambrook J, Russell DW.
PMID: 22485350
CSH Protoc. 2006 Jun 01;2006(1). doi: 10.1101/pdb.prot3883.

No abstract available.

A New Generation Microarray for the Simultaneous Detection and Identification of Yersinia pestis and Bacillus anthracis in Food.

Journal of pathogens

Goji N, Macmillan T, Amoako KK.
PMID: 23125935
J Pathog. 2012;2012:627036. doi: 10.1155/2012/627036. Epub 2012 Oct 18.

The use of microarrays as a multiple analytic system has generated increased interest and provided a powerful analytical tool for the simultaneous detection of pathogens in a single experiment. A wide array of applications for this technology has been...

Messenger RNA Detection in Leukemia Cell lines by Novel Metal-Tagged in situ Hybridization using Inductively Coupled Plasma Mass Spectrometry.

Translational oncogenomics

Ornatsky OI, Baranov VI, Bandura DR, Tanner SD, Dick J.
PMID: 23662035
Transl Oncogenomics. 2006 Sep 14;1:1-9. Print 2006.

Conventional gene expression profiling relies on using fluorescent detection of hybridized probes. Physical characteristics of fluorophores impose limitations on achieving a highly multiplex gene analysis of single cells. Our work demonstrates the feasibility of using metal-tagged in situ hybridization...

Development of novel bioanalytical methods to determine the effective concentrations of phosphorodiamidate morpholino oligomers in tissues and cells.

BioResearch open access

Schnell FJ, Crumley SL, Mourich DV, Iversen PL.
PMID: 23515175
Biores Open Access. 2013 Feb;2(1):61-6. doi: 10.1089/biores.2012.0276.

Phosphorodiamidate morpholino oligomers (PMO) are neutrally charged, sequence-specific antisense agents that interfere with targeted gene expression. PMO have been shown to be highly specific and potent therapies after cellular uptake, yet methods to detect PMO in tissue and inside...

Estimation of bacterial cell numbers in humic acid-rich salt marsh sediments with probes directed to 16S ribosomal DNA .

Applied and environmental microbiology

Devereux R, Edgcomb VP, McDonald JH, Smith DW.
PMID: 10103245
Appl Environ Microbiol. 1999 Apr;65(4):1516-23. doi: 10.1128/AEM.65.4.1516-1523.1999.
Free PMC Article

The feasibility of using probes directed towards ribosomal DNAs (rDNAs) as a quantitative approach to estimating cell numbers was examined and applied to study the structure of a bacterial community in humic acid-rich salt marsh sediments. Hybridizations were performed...

Effect of HLA on development of asthma.

Annals of medicine and surgery (2012)

Mahdi BM, Al-Hadithi ATR, Raouf H, Zalzala HH, Abid LA, Nehad Z.
PMID: 30455876
Ann Med Surg (Lond). 2018 Oct 06;36:118-121. doi: 10.1016/j.amsu.2018.10.003. eCollection 2018 Dec.

BACKGROUND: Asthma is a disease characterized by a chronic inflammation of the airways caused by the interaction of genetic susceptibility with environmental factors. Inflammation and remodeling are critical components of asthma. It is shown that many genes are involved...

Diabetes, metabolic syndrome and obesity : targets and therapy

Chen W, Sun Q, Ju J, Chen W, Zhao X, Zhang Y, Yang Y.
PMID: 30425545
Diabetes Metab Syndr Obes. 2018 Oct 24;11:673-681. doi: 10.2147/DMSO.S177269. eCollection 2018.

INTRODUCTION: Oxidative stress plays an important role in the development of diabetic cardio-myopathy (DCM). Previously, we reported that MATERIALS AND METHODS: H9C2 cells were cultured in the presence of high concentration of glucose or transfected with siRNASOD2, followed by...

Arabis mosaic virus in Grapevines in New York State.

Plant disease

Celebi-Toprak F, Thompson JR, Perry KL, Fuchs M.
PMID: 30722641
Plant Dis. 2013 Jun;97(6):849. doi: 10.1094/PDIS-09-12-0862-PDN.

In a limited survey of commercial vineyards and a germplasm repository in Ontario County, NY, 20 vines of Vitis sp. were tested in fall and spring 2010 to 2012 for viruses using a double-antibody sandwich (DAS)-ELISA and macroarray with...

Showing 25 to 36 of 241 entries