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Showing 13 to 24 of 241 entries
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Characterization of an HSP70 Cognate Gene Family in Arabidopsis.

Plant physiology

Wu CH, Caspar T, Browse J, Lindquist S, Somerville C.
PMID: 16666375
Plant Physiol. 1988 Nov;88(3):731-40. doi: 10.1104/pp.88.3.731.

Analysis of the polypeptide composition of extracts from heat-shocked leaves of Arabidopsis indicated the presence of at least 12 HSP70-related polypeptides, most of which were constitutively expressed. In vitro translation of mRNA from heat-shocked and control leaves indicated that...

Comparing bacterial DNA microarray fingerprints.

Statistical applications in genetics and molecular biology

Willse A, Chandler DP, White A, Protic M, Daly DS, Wunschel S.
PMID: 16646836
Stat Appl Genet Mol Biol. 2005;4:Article19. doi: 10.2202/1544-6115.1162. Epub 2005 Aug 15.

Epidemiologic and forensic investigations often require assays to detect subtle genetic differences between closely related microorganisms. Typically, gel electrophoresis is used to compare randomly amplified DNA fragments between microbial samples, where the patterns of DNA fragment sizes are viewed...

Terminal restriction pattern analysis of 16S rRNA genes for the characterization of bacterial communities of activated sludge.

Journal of bioscience and bioengineering

Hiraishi A, Iwasaki M, Shinjo H.
PMID: 16232834
J Biosci Bioeng. 2000;90(2):148-56. doi: 10.1016/s1389-1723(00)80102-4.

A culture-independent molecular technique using terminal restriction fragment length polymorphism (T-RFLP) of 16S rRNA genes was applied to the characterization of bacterial communities of activated sludge taken from different municipal sewage treatment plants. 16S rDNA fragments from the bulk...

Community analysis of the bacterial assemblages in the winter cover and pelagic layers of a high mountain lake by in situ hybridization.

Applied and environmental microbiology

Alfreider A, Pernthaler J, Amann R, Sattler B, Glockner F, Wille A, Psenner R.
PMID: 16535341
Appl Environ Microbiol. 1996 Jun;62(6):2138-44. doi: 10.1128/aem.62.6.2138-2144.1996.

The bacterial community structure in the winter cover and pelagic zone of a high mountain lake was analyzed by in situ hybridization with fluorescently labeled rRNA-targeted oligonucleotide probes. Cells fixed on membrane filters were hybridized with a probe specific...

Morphological and compositional shifts in an experimental bacterial community influenced by protists with contrasting feeding modes.

Applied and environmental microbiology

Simek K, Vrba J, Pernthaler J, Posch T, Hartman P, Nedoma J, Psenner R.
PMID: 16535515
Appl Environ Microbiol. 1997 Feb;63(2):587-95. doi: 10.1128/aem.63.2.587-595.1997.

In a two-stage continuous-flow system, we studied the impacts of different protozoan feeding modes on the morphology and taxonomic structure of mixed bacterial consortia, which were utilizing organic carbon released by a pure culture of a Rhodomonas sp. grown...

A New Sensitive, Whole-Cell Hybridization Technique for Detection of Bacteria Involving a Biotinylated Oligonucleotide Probe Targeting rRNA and Tyramide Signal Amplification.

Applied and environmental microbiology

Lebaron P, Catala P, Fajon C, Joux F, Baudart J, Bernard L.
PMID: 16535676
Appl Environ Microbiol. 1997 Aug;63(8):3274-8. doi: 10.1128/aem.63.8.3274-3278.1997.

A tyramide signal amplification system with biotinylated oligonucleotide probes and streptavidin-horseradish peroxidase was used to increase the sensitivity of fluorescent in situ hybridization techniques. When applied to both gram-negative and -positive bacteria immobilized on glass slides, a 7- to...

In situ classification and image cytometry of pelagic bacteria from a high mountain lake (gossenkollesee, austria).

Applied and environmental microbiology

Pernthaler J, Alfreider A, Posch T, Andreatta S, Psenner R.
PMID: 16535752
Appl Environ Microbiol. 1997 Dec;63(12):4778-83. doi: 10.1128/aem.63.12.4778-4783.1997.

We describe a procedure to measure the cell sizes of pelagic bacteria after determinative hybridization with rRNA-targeted fluorescently labeled oligonucleotide probes. Our approach is based on established image analysis techniques modified for objects simultaneously stained with two fluorescent dyes....

Group-Specific 16S rRNA-Targeted Oligonucleotide Probes To Identify Thermophilic Bacteria in Marine Hydrothermal Vents.

Applied and environmental microbiology

Harmsen H, Prieur D, Jeanthon C.
PMID: 16535717
Appl Environ Microbiol. 1997 Oct;63(10):4061-8. doi: 10.1128/aem.63.10.4061-4068.1997.

Four 16S rRNA-targeted oligonucleotide probes were designed for the detection of thermophilic members of the domain Bacteria known to thrive in marine hydrothermal systems. We developed and characterized probes encompassing most of the thermophilic members of the genus Bacillus,...

Characterization of the Cricket Hindgut Microbiota with Fluorescently Labeled rRNA-Targeted Oligonucleotide Probes.

Applied and environmental microbiology

Santo Domingo JW, Kaufman MG, Klug MJ, Tiedje JM.
PMID: 16349506
Appl Environ Microbiol. 1998 Feb;64(2):752-5. doi: 10.1128/AEM.64.2.752-755.1998.

Most cricket hindgut microorganisms (60 to 80%) were detected with a universal fluorescent rRNA-targeted probe and found to be eubacteria. Group-specific probes showed that the hindguts of five different cricket species harbor similar bacterial groups, although in different proportions,...

Molecular Cloning and Characterization of c-erb-A mRNA Species in Mouse Neuroblastoma Cells.

Journal of neuroendocrinology

Moeller M, Rapoport B, Gavin LA.
PMID: 19210427
J Neuroendocrinol. 1989 Oct 01;1(5):351-6. doi: 10.1111/j.1365-2826.1989.tb00128.x.

UNLABELLED: Abstract The mouse neuroblastoma cell line is an excellent model in which to study thyroid hormone action and metabolism, particularly in neural tissue. We therefore undertook the molecular cloning and characterization in these cells of putative thyroid hormone...

Design and characterization of two-dye and three-dye binary fluorescent probes for mRNA detection.

Tetrahedron

Martí AA, Li X, Jockusch S, Stevens N, Li Z, Raveendra B, Kalachikov S, Morozova I, Russo JJ, Akins DL, Ju J, Turro NJ.
PMID: 19907676
Tetrahedron. 2007 Apr 23;63(17):3591-3600. doi: 10.1016/j.tet.2006.08.109.

We report the design, synthesis and characterization of binary oligonucleotide probes for mRNA detection. The probes were designed to avoid common problems found in standard binary probes such as direct excitation of the acceptor fluorophore and overlap between the...

Oligonucleotide probes for DNA fingerprinting in horses.

Journal of animal breeding and genetics = Zeitschrift fur Tierzuchtung und Zuchtungsbiologie

Wilke K, Weimann M, Jung M, Geldermann H.
PMID: 21395728
J Anim Breed Genet. 1993 Jan 12;110(1):301-4. doi: 10.1111/j.1439-0388.1993.tb00741.x.

SUMMARY: 10 different oligonucleotide probes were evaluated for DNA fingerprinting in horses. Five probes were able to detect polymorphic bands. The probes (GT)(8) , (GTG)(5) and (GGAT)(4) are most informative for individual identification and were used to analyze a...

Showing 13 to 24 of 241 entries