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Showing 13 to 23 of 23 entries
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Inhibition of Erythroleukemia Cell Growth by Triplex-forming RNAs.

The Ochsner journal

Re RN, Zhang Z, Cook JL.
PMID: 21603516
Ochsner J. 2007;7(2):58-60.

OBJECTIVE: We have previously reported that oligodeoxyribonucleotides, designed to bind in a triplex fashion to a specific p53 binding site homology, inhibit the proliferation of colon cancer cells in vitro and in vivo. The present study was designed to...

Preparation of a disulfide-linked precipitative soluble support for solution-phase synthesis of trimeric oligodeoxyribonucleotide 3´-(2-chlorophenylphosphate) building blocks.

Beilstein journal of organic chemistry

Jabgunde AM, Molina AG, Virta P, Lönnberg H.
PMID: 26664575
Beilstein J Org Chem. 2015 Sep 07;11:1553-60. doi: 10.3762/bjoc.11.171. eCollection 2015.

The preparation of a disulfide-tethered precipitative soluble support and its use for solution-phase synthesis of trimeric oligodeoxyribonucleotide 3´-(2-chlorophenylphosphate) building blocks is described. To obtain the building blocks, N-acyl protected 2´-deoxy-5´-O-(4,4´-dimethoxytrityl)ribonucleosides were phosphorylated with bis(benzotriazol-1-yl) 2-chlorophenyl phosphate. The "outdated" phosphotriester...

Phosphoryl guanidines: a new type of nucleic Acid analogues.

Acta naturae

Kupryushkin MS, Pyshnyi DV, Stetsenko DA.
PMID: 25558402
Acta Naturae. 2014 Oct;6(4):116-8.

A new type of nucleic acid analogues with a phosphoryl guanidine group is described. Oxidation of polymer-supported dinucleoside 2-cyanoethyl phosphite by iodine in the presence of 1,1,3,3-tetramethyl guanidine yields a dinucleotide with an internucleoside tetramethyl phosphoryl guanidine (Tmg) group...

Effects of CpG Oligodeoxynucleotide 1826 on transforming growth factor-beta 1 and radiation-induced pulmonary fibrosis in mice.

Journal of inflammation (London, England)

Li X, Xu G, Qiao T, Yuan S, Zhuang X, Zhang J, Sun HB.
PMID: 27190497
J Inflamm (Lond). 2016 May 17;13:16. doi: 10.1186/s12950-016-0125-4. eCollection 2016.

BACKGROUND: Cytosine-phosphate-guanine (CpG) oligodeoxyribonucleotides (ODNs) are synthetic DNA fragments containing unmethylated cytosine-guanine motifs with potential immune modulatory effects and have recently been suggested to enhance sensitivity to traditional therapies in lung cancer. This study aimed to examine the effects...

Photoactivatable nanoCRISPR/Cas9 System Based on crRNA Reversibly Immobilized on Carbon Nanoparticles.

International journal of molecular sciences

Semikolenova O, Sakovina L, Akhmetova E, Kim D, Vokhtantsev I, Golyshev V, Vorobyeva M, Novopashin S, Novopashina D.
PMID: 34681578
Int J Mol Sci. 2021 Oct 09;22(20). doi: 10.3390/ijms222010919.

Here, we proposed a new approach to engineering a photoactivatable CRISPR/Cas9 gene-editing system. The novel nanoCRISPR/Cas9 system is based on the use of auxiliary photocleavable oligodeoxyribonucleotides (PC-DNAs) complementary to crRNA. PC-DNAs contained up to three UV-sensitive linkers made of...

7',5'-alpha-bicyclo-DNA: new chemistry for oligonucleotide exon splicing modulation therapy.

Nucleic acids research

Evéquoz D, Verhaart IEC, van de Vijver D, Renner W, Aartsma-Rus A, Leumann CJ.
PMID: 34850138
Nucleic Acids Res. 2021 Dec 02;49(21):12089-12105. doi: 10.1093/nar/gkab1097.

Antisense oligonucleotides are small pieces of modified DNA or RNA, which offer therapeutic potential for many diseases. We report on the synthesis of 7',5'-α-bc-DNA phosphoramidite building blocks, bearing the A, G, T and MeC nucleobases. Solid-phase synthesis was performed...

Stereocontrolled Solid-Phase Synthesis of Phosphate/Phosphorothioate (PO/PS) Chimeric Oligodeoxyribonucleotides on an Automated Synthesizer Using an Oxazaphospholidine-Phosphoramidite Method.

The Journal of organic chemistry

Nukaga Y, Oka N, Wada T.
PMID: 26939010
J Org Chem. 2016 Apr 01;81(7):2753-62. doi: 10.1021/acs.joc.5b02793. Epub 2016 Mar 14.

Stereocontrolled solid-phase synthesis of phosphate/phosphorothioate chimeric oligodeoxyribonucleotides (PO/PS-ODNs) was achieved by integrating the conventional phosphoramidite method into a previously developed oxazaphospholidine method for the stereocontrolled synthesis of P-chiral oligonucleotides. P-Stereodefined PO/PS-ODNs with mixed sequences (up to 12-mers) were obtained...

A time- and cost-saving method of producing rat polyclonal antibodies.

Acta histochemica et cytochemica

Wakayama T, Kato Y, Utsumi R, Tsuji A, Iseki S.
PMID: 17327927
Acta Histochem Cytochem. 2006 Jul 01;39(3):79-87. doi: 10.1267/ahc.06003. Epub 2006 Apr 19.

Producing antibodies usually takes more than three months. In the present study, we introduce a faster way of producing polyclonal antibodies based on preparation of the recombinant oligopeptide as antigen followed by immunization of rats. Using this method, we...

Dose-effect relationship of CpG oligodeoxyribonucleotide 1826 in murine Lewis lung cancer treated with irradiation.

OncoTargets and therapy

Zhuang X, Qiao T, Yuan S, Chen W, Zha L, Yan L.
PMID: 23723711
Onco Targets Ther. 2013 May 20;6:549-54. doi: 10.2147/OTT.S42485. Print 2013.

BACKGROUND: Cytosine-phosphate-guanine (CpG) oligodeoxyribonucleotides (ODNs), which induce signaling via Toll-like receptor 9, have recently been suggested to enhance sensitivity to traditional therapies, including chemotherapy, in certain cancer cell lines. This study aimed to define the dose-effect relationship for CpG...

CpG oligodeoxyribonucleotides protect mice from Burkholderia pseudomallei but not Francisella tularensis Schu S4 aerosols.

Journal of immune based therapies and vaccines

Rozak DA, Gelhaus HC, Smith M, Zadeh M, Huzella L, Waag D, Adamovicz JJ.
PMID: 20181102
J Immune Based Ther Vaccines. 2010 Feb 05;8(1):2. doi: 10.1186/1476-8518-8-2.

Studies have shown that CpG oligodeoxyribonucleotides (ODN) protect mice from various bacterial pathogens, including Burkholderia pseudomallei and Francisella tularensis live vaccine strain (LVS), when administered before parenteral challenge. Given the potential to develop CpG ODN as a pre-treatment for...

Structure of the nucleocapsid protein gene of sonchus yellow net virus.

Virology

Zuidema D, Heaton LA, Jackson AO.
PMID: 18644572
Virology. 1987 Aug;159(2):373-80. doi: 10.1016/0042-6822(87)90476-4.

The structure of the gene adjacent to the "leader RNA" gene of sonchus yellow net virus (SYNV), a plant rhabdovirus, was deduced by dideoxyribonucleotide sequence analysis of SYNV genomic (g) RNA and a series of plasmids constructed from SYNV...

Showing 13 to 23 of 23 entries