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Cold-induced phase separation for the simple and reliable extraction of sex hormones for subsequent LC-MS/MS analysis.

Journal of lipid research

Peng R, Le J, Yang SL, Cheng JR, Li Y, Wang ST.
PMID: 34863861
J Lipid Res. 2021 Dec 01;100158. doi: 10.1016/j.jlr.2021.100158. Epub 2021 Dec 01.

Sex hormones, including androgens, estrogens and progestogens, are important biomarkers for various diseases. Quantification of sex hormones is typically conducted by LC-MS/MS. At present, most methods require liquid-liquid extraction (LLE) or solid phase extraction (SPE) for sample preparation. However,...

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Peng R, Le J, Yang SL, et al. Cold-induced phase separation for the simple and reliable extraction of sex hormones for subsequent LC-MS/MS analysis. J Lipid Res. 2021;100158doi: 10.1016/j.jlr.2021.100158.
Peng, R., Le, J., Yang, S. L., Cheng, J. R., Li, Y., & Wang, S. T. (2021). Cold-induced phase separation for the simple and reliable extraction of sex hormones for subsequent LC-MS/MS analysis. Journal of lipid research, 100158. https://doi.org/10.1016/j.jlr.2021.100158
Peng, Rui, et al. "Cold-induced phase separation for the simple and reliable extraction of sex hormones for subsequent LC-MS/MS analysis." Journal of lipid research vol. (2021): 100158. doi: https://doi.org/10.1016/j.jlr.2021.100158
Peng R, Le J, Yang SL, Cheng JR, Li Y, Wang ST. Cold-induced phase separation for the simple and reliable extraction of sex hormones for subsequent LC-MS/MS analysis. J Lipid Res. 2021 Dec 01;100158. doi: 10.1016/j.jlr.2021.100158. Epub 2021 Dec 01. PMID: 34863861.
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Anti-Angiogenic Treatments Interact with Steroid Secretion in Inflammatory Breast Cancer Triple Negative Cell Lines.

Cancers

Alonso-Diez Á, Cáceres S, Peña L, Crespo B, Illera JC.
PMID: 34359570
Cancers (Basel). 2021 Jul 21;13(15). doi: 10.3390/cancers13153668.

Human inflammatory breast cancer (IBC) is a highly angiogenic disease for which antiangiogenic therapy has demonstrated only a modest response, and the reason for this remains unknown. Thus, the purpose of this study was to determine the influence of...

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Alonso-Diez Á, Cáceres S, Peña L, et al. Anti-Angiogenic Treatments Interact with Steroid Secretion in Inflammatory Breast Cancer Triple Negative Cell Lines. Cancers (Basel). 2021;13(15)doi: 10.3390/cancers13153668.
Alonso-Diez, �. �., Cáceres, S., Peña, L., Crespo, B., & Illera, J. C. (2021). Anti-Angiogenic Treatments Interact with Steroid Secretion in Inflammatory Breast Cancer Triple Negative Cell Lines. Cancers, 13(15), . https://doi.org/10.3390/cancers13153668
Alonso-Diez, Ángela, et al. "Anti-Angiogenic Treatments Interact with Steroid Secretion in Inflammatory Breast Cancer Triple Negative Cell Lines." Cancers vol. 13,15 (2021). doi: https://doi.org/10.3390/cancers13153668
Alonso-Diez Á, Cáceres S, Peña L, Crespo B, Illera JC. Anti-Angiogenic Treatments Interact with Steroid Secretion in Inflammatory Breast Cancer Triple Negative Cell Lines. Cancers (Basel). 2021 Jul 21;13(15). doi: 10.3390/cancers13153668. PMID: 34359570; PMCID: PMC8345132.
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The Relationship Between Androgens and Days per Month of Period Pain, Pelvic Pain, Headache, and TLR4 Responsiveness of Peripheral Blood Mononuclear Cells in Young Women with Dysmenorrhoea.

Journal of pain research

Evans SF, Kwok Y, Solterbeck A, Pyragius C, Hull ML, Hutchinson MR, Rolan P.
PMID: 33688248
J Pain Res. 2021 Mar 03;14:585-599. doi: 10.2147/JPR.S279253. eCollection 2021.

PURPOSE: Women bear a disproportionate burden of persistent pain conditions when compared to men. To determine whether the hormonal environment affects the clinical experience of pain, as measured by the days per month of pelvic pain (DPelvicPM), period pain...

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Evans SF, Kwok Y, Solterbeck A, et al. The Relationship Between Androgens and Days per Month of Period Pain, Pelvic Pain, Headache, and TLR4 Responsiveness of Peripheral Blood Mononuclear Cells in Young Women with Dysmenorrhoea. J Pain Res. 2021;14:585-599doi: 10.2147/JPR.S279253.
Evans, S. F., Kwok, Y., Solterbeck, A., Pyragius, C., Hull, M. L., Hutchinson, M. R., & Rolan, P. (2021). The Relationship Between Androgens and Days per Month of Period Pain, Pelvic Pain, Headache, and TLR4 Responsiveness of Peripheral Blood Mononuclear Cells in Young Women with Dysmenorrhoea. Journal of pain research, 14585-599. https://doi.org/10.2147/JPR.S279253
Evans, Susan F, et al. "The Relationship Between Androgens and Days per Month of Period Pain, Pelvic Pain, Headache, and TLR4 Responsiveness of Peripheral Blood Mononuclear Cells in Young Women with Dysmenorrhoea." Journal of pain research vol. 14 (2021): 585-599. doi: https://doi.org/10.2147/JPR.S279253
Evans SF, Kwok Y, Solterbeck A, Pyragius C, Hull ML, Hutchinson MR, Rolan P. The Relationship Between Androgens and Days per Month of Period Pain, Pelvic Pain, Headache, and TLR4 Responsiveness of Peripheral Blood Mononuclear Cells in Young Women with Dysmenorrhoea. J Pain Res. 2021 Mar 03;14:585-599. doi: 10.2147/JPR.S279253. eCollection 2021. PMID: 33688248; PMCID: PMC7937378.
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Steroid hormone profiles and body conditions of migrating male humpback whales (Megaptera novaeangliae).

General and comparative endocrinology

Dalle Luche G, Boggs ASP, Kucklick JR, Hawker DW, Wisse JH, Bengtson Nash S.
PMID: 34425085
Gen Comp Endocrinol. 2021 Nov 01;313:113888. doi: 10.1016/j.ygcen.2021.113888. Epub 2021 Aug 20.

Simultaneous analysis of multiple steroid hormones from remotely obtained blubber biopsies has the potential to concurrently provide information regarding stress and reproductive status from free-swimming cetaceans, while also investigating correlations between hormone concentrations and other health biomarkers. In this...

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Dalle Luche G, Boggs ASP, Kucklick JR, et al. Steroid hormone profiles and body conditions of migrating male humpback whales (Megaptera novaeangliae). Gen Comp Endocrinol. 2021;313:113888doi: 10.1016/j.ygcen.2021.113888.
Dalle Luche, G., Boggs, A. S. P., Kucklick, J. R., Hawker, D. W., Wisse, J. H., & Bengtson Nash, S. (2021). Steroid hormone profiles and body conditions of migrating male humpback whales (Megaptera novaeangliae). General and comparative endocrinology, 313113888. https://doi.org/10.1016/j.ygcen.2021.113888
Dalle Luche, Greta, et al. "Steroid hormone profiles and body conditions of migrating male humpback whales (Megaptera novaeangliae)." General and comparative endocrinology vol. 313 (2021): 113888. doi: https://doi.org/10.1016/j.ygcen.2021.113888
Dalle Luche G, Boggs ASP, Kucklick JR, Hawker DW, Wisse JH, Bengtson Nash S. Steroid hormone profiles and body conditions of migrating male humpback whales (Megaptera novaeangliae). Gen Comp Endocrinol. 2021 Nov 01;313:113888. doi: 10.1016/j.ygcen.2021.113888. Epub 2021 Aug 20. PMID: 34425085.
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Circulating Sex Hormones and Risk of Colorectal Adenomas and Serrated Lesions in Men.

Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology

Figueiredo JC, Gresham G, Barry EL, Mott LA, Passarelli MN, Bradshaw PT, Anderson CW, Baron JA.
PMID: 34758968
Cancer Epidemiol Biomarkers Prev. 2022 Jan;31(1):293-295. doi: 10.1158/1055-9965.EPI-21-0713. Epub 2021 Nov 10.

BACKGROUND: Sex hormones have been implicated in the etiology of colorectal neoplasia in women for over 40 years, but there has been very little investigation of the role of these hormones in men.METHODS: Using data from an adenoma chemoprevention...

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Figueiredo JC, Gresham G, Barry EL, et al. Circulating Sex Hormones and Risk of Colorectal Adenomas and Serrated Lesions in Men. Cancer Epidemiol Biomarkers Prev. 2021;31(1):293-295doi: 10.1158/1055-9965.EPI-21-0713.
Figueiredo, J. C., Gresham, G., Barry, E. L., Mott, L. A., Passarelli, M. N., Bradshaw, P. T., Anderson, C. W., & Baron, J. A. (2022). Circulating Sex Hormones and Risk of Colorectal Adenomas and Serrated Lesions in Men. Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, 31(1), 293-295. https://doi.org/10.1158/1055-9965.EPI-21-0713
Figueiredo, Jane C, et al. "Circulating Sex Hormones and Risk of Colorectal Adenomas and Serrated Lesions in Men." Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology vol. 31,1 (2022): 293-295. doi: https://doi.org/10.1158/1055-9965.EPI-21-0713
Figueiredo JC, Gresham G, Barry EL, Mott LA, Passarelli MN, Bradshaw PT, Anderson CW, Baron JA. Circulating Sex Hormones and Risk of Colorectal Adenomas and Serrated Lesions in Men. Cancer Epidemiol Biomarkers Prev. 2022 Jan;31(1):293-295. doi: 10.1158/1055-9965.EPI-21-0713. Epub 2021 Nov 10. PMID: 34758968; PMCID: PMC8755601.
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Ovarian Steroid Cell Tumor Masquerading as Steroid-Unresponsive Congenital Adrenal Hyperplasia.

AACE clinical case reports

Driano JE, Creo AL, Kumar S, Chattha AJ, Lteif AN.
PMID: 34307849
AACE Clin Case Rep. 2021 Feb 17;7(4):261-263. doi: 10.1016/j.aace.2021.02.001. eCollection 2021.

OBJECTIVE: Ovarian neoplasms in children are rare. The objective of this report is to emphasize the importance of considering those neoplasms in the differential diagnosis of hyperandrogenism even with negative diagnostic imaging.METHODS: We report the case of a 12-year-old...

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Driano JE, Creo AL, Kumar S, et al. Ovarian Steroid Cell Tumor Masquerading as Steroid-Unresponsive Congenital Adrenal Hyperplasia. AACE Clin Case Rep. 2021;7(4):261-263doi: 10.1016/j.aace.2021.02.001.
Driano, J. E., Creo, A. L., Kumar, S., Chattha, A. J., & Lteif, A. N. (2021). Ovarian Steroid Cell Tumor Masquerading as Steroid-Unresponsive Congenital Adrenal Hyperplasia. AACE clinical case reports, 7(4), 261-263. https://doi.org/10.1016/j.aace.2021.02.001
Driano, Jane E, et al. "Ovarian Steroid Cell Tumor Masquerading as Steroid-Unresponsive Congenital Adrenal Hyperplasia." AACE clinical case reports vol. 7,4 (2021): 261-263. doi: https://doi.org/10.1016/j.aace.2021.02.001
Driano JE, Creo AL, Kumar S, Chattha AJ, Lteif AN. Ovarian Steroid Cell Tumor Masquerading as Steroid-Unresponsive Congenital Adrenal Hyperplasia. AACE Clin Case Rep. 2021 Feb 17;7(4):261-263. doi: 10.1016/j.aace.2021.02.001. eCollection 2021. PMID: 34307849; PMCID: PMC8282522.
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Comprehensive sex steroid profiling in multiple tissues reveals novel insights in sex steroid distribution in male mice.

Endocrinology

Colldén H, Nilsson ME, Norlén AK, Landin A, Windahl SH, Wu J, Gustafsson KL, Poutanen M, Ryberg H, Vandenput L, Ohlsson C.
PMID: 34999782
Endocrinology. 2022 Jan 06; doi: 10.1210/endocr/bqac001. Epub 2022 Jan 06.

A comprehensive atlas of sex steroid distribution in multiple tissues is currently lacking and how circulating and tissue sex steroid levels correlate remains unknown. Here, we adapted and validated a gas chromatography-tandem mass spectrometry method for simultaneous measurement of...

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Colldén H, Nilsson ME, Norlén AK, et al. Comprehensive sex steroid profiling in multiple tissues reveals novel insights in sex steroid distribution in male mice. Endocrinology. 2022;doi: 10.1210/endocr/bqac001.
Colldén, H., Nilsson, M. E., Norlén, A. K., Landin, A., Windahl, S. H., Wu, J., Gustafsson, K. L., Poutanen, M., Ryberg, H., Vandenput, L., & Ohlsson, C. (2022). Comprehensive sex steroid profiling in multiple tissues reveals novel insights in sex steroid distribution in male mice. Endocrinology, . https://doi.org/10.1210/endocr/bqac001
Colldén, Hannah, et al. "Comprehensive sex steroid profiling in multiple tissues reveals novel insights in sex steroid distribution in male mice." Endocrinology vol. (2022). doi: https://doi.org/10.1210/endocr/bqac001
Colldén H, Nilsson ME, Norlén AK, Landin A, Windahl SH, Wu J, Gustafsson KL, Poutanen M, Ryberg H, Vandenput L, Ohlsson C. Comprehensive sex steroid profiling in multiple tissues reveals novel insights in sex steroid distribution in male mice. Endocrinology. 2022 Jan 06; doi: 10.1210/endocr/bqac001. Epub 2022 Jan 06. PMID: 34999782.
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A Prospective Study of the Relationship Between Clinical Outcomes After Enzalutamide and Serum Androgen Levels Measured via Liquid Chromatography-tandem Mass Spectrometry in Patients with Castration-resistant Prostate Cancer.

European urology open science

Miyazawa Y, Sekine Y, Arai S, Nakamura T, Takezawa Y, Shimizu N, Matsuo Y, Ogura H, Takei T, Suzuki K.
PMID: 34337535
Eur Urol Open Sci. 2021 Jun 07;29:59-67. doi: 10.1016/j.euros.2021.05.003. eCollection 2021 Jul.

BACKGROUND: Enzalutamide (ENZ) is used to treat patients with castration-resistant prostate cancer (CRPC). However, the kinetics of serum androgens before and after ENZ treatment are unknown.OBJECTIVE: To elucidate the kinetics of serum androgens and explore the possibility of identifying...

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Miyazawa Y, Sekine Y, Arai S, et al. A Prospective Study of the Relationship Between Clinical Outcomes After Enzalutamide and Serum Androgen Levels Measured via Liquid Chromatography-tandem Mass Spectrometry in Patients with Castration-resistant Prostate Cancer. Eur Urol Open Sci. 2021;29:59-67doi: 10.1016/j.euros.2021.05.003.
Miyazawa, Y., Sekine, Y., Arai, S., Nakamura, T., Takezawa, Y., Shimizu, N., Matsuo, Y., Ogura, H., Takei, T., & Suzuki, K. (2021). A Prospective Study of the Relationship Between Clinical Outcomes After Enzalutamide and Serum Androgen Levels Measured via Liquid Chromatography-tandem Mass Spectrometry in Patients with Castration-resistant Prostate Cancer. European urology open science, 2959-67. https://doi.org/10.1016/j.euros.2021.05.003
Miyazawa, Yoshiyuki, et al. "A Prospective Study of the Relationship Between Clinical Outcomes After Enzalutamide and Serum Androgen Levels Measured via Liquid Chromatography-tandem Mass Spectrometry in Patients with Castration-resistant Prostate Cancer." European urology open science vol. 29 (2021): 59-67. doi: https://doi.org/10.1016/j.euros.2021.05.003
Miyazawa Y, Sekine Y, Arai S, Nakamura T, Takezawa Y, Shimizu N, Matsuo Y, Ogura H, Takei T, Suzuki K. A Prospective Study of the Relationship Between Clinical Outcomes After Enzalutamide and Serum Androgen Levels Measured via Liquid Chromatography-tandem Mass Spectrometry in Patients with Castration-resistant Prostate Cancer. Eur Urol Open Sci. 2021 Jun 07;29:59-67. doi: 10.1016/j.euros.2021.05.003. eCollection 2021 Jul. PMID: 34337535; PMCID: PMC8317787.
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Turner syndrome with positive .

World journal of clinical cases

He MN, Zhao SC, Li JM, Tong LL, Fan XZ, Xue YM, Lin XH, Cao Y.
PMID: 33869601
World J Clin Cases. 2021 Apr 06;9(10):2259-2267. doi: 10.12998/wjcc.v9.i10.2259.

BACKGROUND: Co-morbidity of CASE SUMMARY: In this article, we present a 14-year-old girl who was referred to our hospital with short stature (weight of 43 kg and height of 143 cm, < -2 SD) with no secondary sexual characteristics...

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He MN, Zhao SC, Li JM, et al. Turner syndrome with positive . World J Clin Cases. 2021;9(10):2259-2267doi: 10.12998/wjcc.v9.i10.2259.
He, M. N., Zhao, S. C., Li, J. M., Tong, L. L., Fan, X. Z., Xue, Y. M., Lin, X. H., & Cao, Y. (2021). Turner syndrome with positive . World journal of clinical cases, 9(10), 2259-2267. https://doi.org/10.12998/wjcc.v9.i10.2259
He, Mei-Nan, et al. "Turner syndrome with positive ." World journal of clinical cases vol. 9,10 (2021): 2259-2267. doi: https://doi.org/10.12998/wjcc.v9.i10.2259
He MN, Zhao SC, Li JM, Tong LL, Fan XZ, Xue YM, Lin XH, Cao Y. Turner syndrome with positive . World J Clin Cases. 2021 Apr 06;9(10):2259-2267. doi: 10.12998/wjcc.v9.i10.2259. PMID: 33869601; PMCID: PMC8026834.
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Sphingolipid profiles and their relationship with inflammatory factors in asthmatic patients of different sexes.

Chronic diseases and translational medicine

Song Z, Yan W, Abulikemu M, Wang J, Xing Y, Zhou Q, Ma S, Chang C.
PMID: 34505020
Chronic Dis Transl Med. 2021 May 13;7(3):199-205. doi: 10.1016/j.cdtm.2021.04.002. eCollection 2021 Sep.

BACKGROUND: Asthma is a heterogeneous disease with distinct prevalence and manifestation between sexes. This study was to identify sex-specific features of asthma via metabolomic analysis of sphingolipids.METHODS: Forty-two asthma patients (27 women and 15 men) admitted to the Peking...

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Song Z, Yan W, Abulikemu M, et al. Sphingolipid profiles and their relationship with inflammatory factors in asthmatic patients of different sexes. Chronic Dis Transl Med. 2021;7(3):199-205doi: 10.1016/j.cdtm.2021.04.002.
Song, Z., Yan, W., Abulikemu, M., Wang, J., Xing, Y., Zhou, Q., Ma, S., & Chang, C. (2021). Sphingolipid profiles and their relationship with inflammatory factors in asthmatic patients of different sexes. Chronic diseases and translational medicine, 7(3), 199-205. https://doi.org/10.1016/j.cdtm.2021.04.002
Song, Zhu, et al. "Sphingolipid profiles and their relationship with inflammatory factors in asthmatic patients of different sexes." Chronic diseases and translational medicine vol. 7,3 (2021): 199-205. doi: https://doi.org/10.1016/j.cdtm.2021.04.002
Song Z, Yan W, Abulikemu M, Wang J, Xing Y, Zhou Q, Ma S, Chang C. Sphingolipid profiles and their relationship with inflammatory factors in asthmatic patients of different sexes. Chronic Dis Transl Med. 2021 May 13;7(3):199-205. doi: 10.1016/j.cdtm.2021.04.002. eCollection 2021 Sep. PMID: 34505020; PMCID: PMC8413120.
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Exposure of zebrafish larvae to water accommodated fractions of weathered crude oil alters steroid hormone concentrations with minimal effect on cholesterol.

Aquatic toxicology (Amsterdam, Netherlands)

Price ER, Bonatesta F, McGruer V, Schlenk D, Roberts AP, Mager EM.
PMID: 34871821
Aquat Toxicol. 2021 Nov 27;242:106045. doi: 10.1016/j.aquatox.2021.106045. Epub 2021 Nov 27.

Crude oil has multiple toxic effects in fish, particularly during their early life stages. Recent transcriptomics studies have highlighted a potential effect on cholesterol homeostasis and biosynthesis, but have not investigated effects on steroid hormones, which are biosynthetically downstream...

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Price ER, Bonatesta F, McGruer V, et al. Exposure of zebrafish larvae to water accommodated fractions of weathered crude oil alters steroid hormone concentrations with minimal effect on cholesterol. Aquat Toxicol. 2021;242:106045doi: 10.1016/j.aquatox.2021.106045.
Price, E. R., Bonatesta, F., McGruer, V., Schlenk, D., Roberts, A. P., & Mager, E. M. (2021). Exposure of zebrafish larvae to water accommodated fractions of weathered crude oil alters steroid hormone concentrations with minimal effect on cholesterol. Aquatic toxicology (Amsterdam, Netherlands), 242106045. https://doi.org/10.1016/j.aquatox.2021.106045
Price, Edwin R, et al. "Exposure of zebrafish larvae to water accommodated fractions of weathered crude oil alters steroid hormone concentrations with minimal effect on cholesterol." Aquatic toxicology (Amsterdam, Netherlands) vol. 242 (2021): 106045. doi: https://doi.org/10.1016/j.aquatox.2021.106045
Price ER, Bonatesta F, McGruer V, Schlenk D, Roberts AP, Mager EM. Exposure of zebrafish larvae to water accommodated fractions of weathered crude oil alters steroid hormone concentrations with minimal effect on cholesterol. Aquat Toxicol. 2021 Nov 27;242:106045. doi: 10.1016/j.aquatox.2021.106045. Epub 2021 Nov 27. PMID: 34871821.
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