J Immunol. 1975 Aug;115(2):564-8.

Immunochemical analysis of a Smith-like antigen isolated from two human strains of Staphylococcus aureus.

Journal of immunology (Baltimore, Md. : 1950)

W W Karakawa, J A Kane

PMID: 50368

Abstract

A surface antigen consisting of aminoglucuronic acid and N-acetyl-L-alanine was isolated from the culture filtrates of two human strains of Staphylococcus aureus. Double diffusion analysis in agar suggested that the antigen is immunologically similar to the alanyl-aminoglucuronic acid capsule of the Smith strain of S. aureus. Quantitative precipitin inhibition studies indicated that N-acetyl-L-alanine is the immunodominant determinant of the acidic antigen. In addition, conjugates consisting of N-acetyl-L-alanine coupled to bovine serum albumin gave a significant precipitin reaction with anti-staphylococcal serum which is rich in alanyl-aminoglucuronic acid polymer antibodies. Antibodies with N-acetyl-L-alanine specificity were isolated from N-acetyl-L-alanine-Sepharose immunoabsorbent columns. Double diffusion analysis in agar indicated that the eluted antibodies were serologically reactive and belonged to the IgG class of immunoglobulins.

Substances

• Amino Acids
• Amino Sugars
• Antibodies, Bacterial
• Antigens, Bacterial
• Epitopes
• Glucuronates
• Immune Sera
• Uronic Acids
• N-acetylalanine
• Phosphorus
• Alanine

MeSH terms

• Absorption
• Alanine / analogs & derivatives
• Amino Acids / analysis
• Amino Sugars / analysis
• Animals
• Antibodies, Bacterial / analysis
• Antigens, Bacterial / analysis
• Cell Wall / immunology
• Chromatography, Affinity
• Chromatography, Gas
• Chromatography, Ion Exchange
• Epitopes
• Erythrocytes / immunology
• Glucuronates / analysis
• Hemagglutination Tests
• Humans
• Immune Sera
• Immunochemistry
• Immunodiffusion
• Immunologic Techniques
• Phosphorus / analysis
• Sheep / immunology
• Staphylococcus / immunology
• Uronic Acids / analysis

Publication Types

• Journal Article
• Research Support, U.S. Gov't, P.H.S.

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