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Ellis-Davies GC, Kaplan JH, Barsotti RJ. Laser photolysis of caged calcium: rates of calcium release by nitrophenyl-EGTA and DM-nitrophen. Biophys J. 1996;70(2):1006-16doi: 10.1016/S0006-3495(96)79644-3.
Ellis-Davies, G. C., Kaplan, J. H., & Barsotti, R. J. (1996). Laser photolysis of caged calcium: rates of calcium release by nitrophenyl-EGTA and DM-nitrophen. Biophysical journal, 70(2), 1006-16. https://doi.org/10.1016/S0006-3495(96)79644-3
Ellis-Davies, G C, et al. "Laser photolysis of caged calcium: rates of calcium release by nitrophenyl-EGTA and DM-nitrophen." Biophysical journal vol. 70,2 (1996): 1006-16. doi: https://doi.org/10.1016/S0006-3495(96)79644-3
Ellis-Davies GC, Kaplan JH, Barsotti RJ. Laser photolysis of caged calcium: rates of calcium release by nitrophenyl-EGTA and DM-nitrophen. Biophys J. 1996 Feb;70(2):1006-16. doi: 10.1016/S0006-3495(96)79644-3. PMID: 8789118; PMCID: PMC1225001.
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Elsevier Science Free PMC Article

Biophys J. 1996 Feb;70(2):1006-16. doi: 10.1016/S0006-3495(96)79644-3.

Laser photolysis of caged calcium: rates of calcium release by nitrophenyl-EGTA and DM-nitrophen.

Biophysical journal

G C Ellis-Davies, J H Kaplan, R J Barsotti

Affiliations

  1. Department of Biochemistry and Molecular Biology, Oregon Health Sciences University, Portland 97201, USA.

PMID: 8789118 PMCID: PMC1225001 DOI: 10.1016/S0006-3495(96)79644-3
Free PMC Article

Abstract

Nitrophenyl-EGTA and DM-nitrophen are Ca2+ cages that release Ca2+ when cleaved upon illumination with near-ultraviolet light. Laser photolysis of nitrophenyl-EGTA produced transient intermediates that decayed biexponentially with rates of 500,000 s-1 and 100,000 s-1 in the presence of saturating Ca2+ and 290,000 s-1 and 68,000 s-1 in the absence of Ca2+ at pH 7.2 and 25 degrees C. Laser photolysis of nitrophenyl-EGTA in the presence of Ca2+ and the Ca2+ indicator Ca-orange-5N produced a monotonic increase in the indicator fluorescence, which had a rate of 68,000 s-1 at pH 7.2 and 25 degrees C. Irradiation of DM-nitrophen produced similar results with somewhat slower kinetics. The transient intermediates decayed with rates of 80,000 s-1 and 11,000 s-1 in the presence of Ca2+ and 59,000 s-1 and 3,600 s-1 in the absence of Ca2+ at pH 7.2 and 25 degrees C. The rate of increase in Ca(2+)-indicator fluorescence produced upon photolysis of the DM-nitrophen: Ca2+ complex was 38,000 s-1 at pH 7.2 and 25 degrees C. In contrast, pulses in Ca2+ concentration were generated when the chelator concentrations were more than the total Ca2+ concentration. Photoreleased Ca2+ concentration stabilized under these circumstances to a steady state within 1-2 ms.

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