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Hui P, Guo X, Bradford PG. Isolation and functional characterization of the human gene encoding the myeloid zinc finger protein MZF-1. Biochemistry. 1995;34(50):16493-502doi: 10.1021/bi00050a033.
Hui, P., Guo, X., & Bradford, P. G. (1995). Isolation and functional characterization of the human gene encoding the myeloid zinc finger protein MZF-1. Biochemistry, 34(50), 16493-502. https://doi.org/10.1021/bi00050a033
Hui, P, et al. "Isolation and functional characterization of the human gene encoding the myeloid zinc finger protein MZF-1." Biochemistry vol. 34,50 (1995): 16493-502. doi: https://doi.org/10.1021/bi00050a033
Hui P, Guo X, Bradford PG. Isolation and functional characterization of the human gene encoding the myeloid zinc finger protein MZF-1. Biochemistry. 1995 Dec 19;34(50):16493-502. doi: 10.1021/bi00050a033. PMID: 8845378.
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Biochemistry. 1995 Dec 19;34(50):16493-502. doi: 10.1021/bi00050a033.

Isolation and functional characterization of the human gene encoding the myeloid zinc finger protein MZF-1.

Biochemistry

P Hui, X Guo, P G Bradford

Affiliations

  1. Department of Pharmacology and Toxicology, School of Medicine and Biomedical Sciences, State University of New York at Buffalo 14214-3000, USA.

PMID: 8845378 DOI: 10.1021/bi00050a033

Abstract

The expression of the human myeloid zinc finger gene (MZF-1) by human bone marrow cells is necessary for granulopoiesis. We have analyzed the structure and function of the MZF-1 gene by diagnostic polymerase chain reaction, genomic cloning, and promoter analysis. Comparison of human promyelocytic HL-60 cell cDNA with isolated MZF-1 genomic clones indicated that the human MZF-1 gene is without introns and spans approximately 3 kb. Restriction enzyme mapping and Southern analysis indicated further that the human MZF-1 gene is a single-copy gene. Primer extension studies identified the major transcription start site as a thymidine residue located 1102 bp upstream of the ATG translation start codon. A putative TATA box sequence (TAAAAA) was found at -66 bp and a CCAAT box at -130 bp relative to the transcription initiation site. In HL-60 cells, MZF-1 mRNA levels are increased by granulopoietic inducers including retinoic acid and GM-CSF. DNA upstream of the transcription start site contains tandem-repeated consensus retinoic acid response elements at -666 through -696 bp and paired putative GM-CSF-responsive sequences centered at -50 and -100 bp. CAT reporter gene constructs containing these DNA regions promoted transcription and conferred transcriptional responsiveness to retinoic acid and GM-CSF when transfected into HL-60 cells. Additional putative regulatory binding sites included conserved MZF-1 zinc finger binding sequences, the importance of which was suggested by the enhanced expression of the endogenous MZF-1 gene following vector-driven expression of MZF-1 constructs in K562 myeloblastic leukemia cells. These findings provide a clearer basis for understanding the role of MZF-1 gene expression in myeloid cell growth and differentiation.

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