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Armiger LC, Headrick JP, Jordan LR, et al. Bound inorganic phosphate and early contractile failure in global ischaemia. Basic Res Cardiol. 1995;90(6):482-8doi: 10.1007/BF00788541.
Armiger, L. C., Headrick, J. P., Jordan, L. R., & Willis, R. J. (1995). Bound inorganic phosphate and early contractile failure in global ischaemia. Basic research in cardiology, 90(6), 482-8. https://doi.org/10.1007/BF00788541
Armiger, L C, et al. "Bound inorganic phosphate and early contractile failure in global ischaemia." Basic research in cardiology vol. 90,6 (1995): 482-8. doi: https://doi.org/10.1007/BF00788541
Armiger LC, Headrick JP, Jordan LR, Willis RJ. Bound inorganic phosphate and early contractile failure in global ischaemia. Basic Res Cardiol. 1995 Nov-Dec;90(6):482-8. doi: 10.1007/BF00788541. PMID: 8773193.
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Basic Res Cardiol. 1995 Nov-Dec;90(6):482-8. doi: 10.1007/BF00788541.

Bound inorganic phosphate and early contractile failure in global ischaemia.

Basic research in cardiology

L C Armiger, J P Headrick, L R Jordan, R J Willis

Affiliations

  1. Department of Pathology, University of Auckland, New Zeland.

PMID: 8773193 DOI: 10.1007/BF00788541

Abstract

Inorganic phosphate (Pi) accumulates extremely rapidly in ischaemic heart muscle and intracellular binding of this metabolite may account for the precipitous loss of function seen at the onset of severe ischaemia. We have used 31P-NMR spectroscopy to measure the free cytosolic [Pi] and chemical assay techniques to measure total tissue Pi at 0, 1, 2, 3, 4, 5, and 12 min of complete global ischaemia in the isolated isovolumic rat heart. At zero time, the Pi assayed chemically was 30.77 +/- 5.52 mumol/g dry wt (mean +/- SD, n = 7) whilst Pi assayed by NMR was 3.39 +/- 1.21 mumol/g dry wt (n = 15). Thus, 27.38 mumol/g dry wt of Pi was bound at a cytosolic [Pi] of 0.82 mM. After 12 min of ischaemia, 49.88 mumol/g dry wt of Pi was bound at a cytosolic [Pi] of 4.11 mM. When all data were fitted, using a non-linear, least squares fit (p < 0.05), to the binding isotherm: Bound Pi = Bmax'. [Pi]/Kd'+[Pi], the apparent binding parameters Kd' and Bmax' were estimated to be 1.1 +/- 0.6 mM and 64.0 +/- 10.2 mumol/g dry wt respectively. During the first minute of global ischaemia when the rate-pressure product had decreased by 79% of its pre-ischaemic value, bound Pi had increased by 58% and free cytosolic [Pi] by 162%. When functional and metabolite changes were expressed as a fraction of the total change which occurred during the 12-min ischaemic period, bound Pi had the profile most similar to the rate-pressure product. Both the amount of bound Pi and free cytosolic [Pi] correlated with loss of contractile function as the ischaemic period progressed. The results show that during ischaemia, Pi is bound progressively as free cytosolic [Pi] is increased as the result of high energy phosphate hydrolysis. While these results are consistent with the possibility that Pi binding may contribute to ischaemic contractile failure, no molecular explanation for the possible effect of bound Pi on contraction has been proposed.

References

  1. Am J Physiol. 1990 Jun;258(6 Pt 1):C967-81 - PubMed
  2. Am J Physiol. 1983 Nov;245(5 Pt 1):C423-7 - PubMed
  3. Biochem Med. 1983 Jun;29(3):265-77 - PubMed
  4. Heart Vessels. 1988;4(4):207-14 - PubMed
  5. Heart Vessels. 1986;2(1):6-14 - PubMed
  6. J Physiol. 1992 Aug;454:467-90 - PubMed
  7. Am J Physiol. 1987 Aug;253(2 Pt 2):H412-21 - PubMed
  8. J Mol Cell Cardiol. 1990 Oct;22(10):1107-16 - PubMed
  9. J Mol Cell Cardiol. 1985 Aug;17(8):747-52 - PubMed
  10. Eur J Biochem. 1990 Aug 17;191(3):755-9 - PubMed
  11. Am J Cardiol. 1977 Jun;39(7):986-93 - PubMed
  12. Circ Res. 1991 Jan;68(1):255-62 - PubMed
  13. Am J Physiol. 1994 Sep;267(3 Pt 2):H1074-84 - PubMed
  14. J Physiol. 1986 Jan;370:585-604 - PubMed
  15. J Mol Cell Cardiol. 1982 May;14 (5):267-77 - PubMed
  16. Am J Physiol. 1982 May;242(5):H729-44 - PubMed
  17. Am J Physiol. 1991 Jan;260(1 Pt 2):H6-12 - PubMed
  18. J Mol Cell Cardiol. 1983 Dec;15(12):855-8 - PubMed
  19. Circ Res. 1986 Sep;59(3):270-82 - PubMed
  20. J Biochem. 1988 Jul;104(1):35-9 - PubMed

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