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Méténier L, Kaminski M. Etude immunochimique de l'expression génétique de la carboxylestérase du sérum de cheval. [Immunochemical investigations on the gene expression of horse serum carboxylesterase (author's transl)]. Ann Immunol (Paris). 1978;129(2):353-65
Méténier, L., & Kaminski, M. (1978). Etude immunochimique de l'expression génétique de la carboxylestérase du sérum de cheval. [Immunochemical investigations on the gene expression of horse serum carboxylesterase (author's transl)]. Annales d'immunologie, 129(2), 353-65.
Méténier, L, and Kaminski, M. "Etude immunochimique de l'expression génétique de la carboxylestérase du sérum de cheval." [Immunochemical investigations on the gene expression of horse serum carboxylesterase (author's transl)]. Annales d'immunologie vol. 129,2 (1978): 353-65.
Méténier L, Kaminski M. Etude immunochimique de l'expression génétique de la carboxylestérase du sérum de cheval. [Immunochemical investigations on the gene expression of horse serum carboxylesterase (author's transl)]. Ann Immunol (Paris). 1978 Feb-Mar;129(2):353-65. French. PMID: 79331.
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Ann Immunol (Paris). 1978 Feb-Mar;129(2):353-65.

[Immunochemical investigations on the gene expression of horse serum carboxylesterase (author's transl)].

Annales d'immunologie

[Article in French]
L Méténier, M Kaminski

PMID: 79331

Abstract

Immunochemical and enzymatic analyses of horse serum carboxylesterase were carried out with respect to the existence of a silent gene. Sera with positive phenotypic expression of esterase, both heterozygotes and presumed homozygotes, were compared with:--sera with positive phenotypic expression but genotypically +/O;--sera with a negative phenotypic expression, i. e. genotypically O/O;--sera of natural +/O "hemi-zygotes": mules (donkey lacking the esterase);--positive sera heated at 60 degrees C;--positive sera after specific inhibition of enzymatic activity. Titration by immunocompetition has shown that sera phenotypically negative do not contain an immunologically active "dummy" protein; however, using a sensitive immunotitration (rocket immunoelectrophoresis), traces of active protein could be demonstrated. Sera of +/O genotypes, both horses and mules, contain approximatively half of esterase present in +/+ sera. Heating at 60 degrees destroys the antigenic properties of the esterase almost completely, while, on the contrary, a specific inhibition of enzymatic activity leaves the antigenic properties unaffected.

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