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Richter D, Incani RN, Harn DA. Isotype responses to candidate vaccine antigens in protective sera obtained from mice vaccinated with irradiated cercariae of Schistosoma mansoni. Infect Immun. 1993;61(7):3003-11doi: 10.1128/iai.61.7.3003-3011.1993.
Richter, D., Incani, R. N., & Harn, D. A. (1993). Isotype responses to candidate vaccine antigens in protective sera obtained from mice vaccinated with irradiated cercariae of Schistosoma mansoni. Infection and immunity, 61(7), 3003-11. https://doi.org/10.1128/iai.61.7.3003-3011.1993
Richter, D, et al. "Isotype responses to candidate vaccine antigens in protective sera obtained from mice vaccinated with irradiated cercariae of Schistosoma mansoni." Infection and immunity vol. 61,7 (1993): 3003-11. doi: https://doi.org/10.1128/iai.61.7.3003-3011.1993
Richter D, Incani RN, Harn DA. Isotype responses to candidate vaccine antigens in protective sera obtained from mice vaccinated with irradiated cercariae of Schistosoma mansoni. Infect Immun. 1993 Jul;61(7):3003-11. doi: 10.1128/iai.61.7.3003-3011.1993. PMID: 8514407; PMCID: PMC280952.
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Atypon Free PMC Article

Infect Immun. 1993 Jul;61(7):3003-11. doi: 10.1128/iai.61.7.3003-3011.1993.

Isotype responses to candidate vaccine antigens in protective sera obtained from mice vaccinated with irradiated cercariae of Schistosoma mansoni.

Infection and immunity

D Richter, R N Incani, D A Harn

Affiliations

  1. Department of Tropical Public Health, Harvard School of Public Health, Boston, Massachusetts 02115.

PMID: 8514407 PMCID: PMC280952 DOI: 10.1128/iai.61.7.3003-3011.1993
Free PMC Article

Abstract

In experimental schistosomiasis, sera of mice multiply vaccinated with radiation-attenuated cercariae of Schistosoma mansoni passively transfer resistance against cercarial challenge to naive mice. To further characterize these sera, we tested their protective capacities in two mouse strains (C57BL/6J and CBA/J) and compared the antigen-specific isotype compositions of the different protective sera by means of the enzyme-linked immunosorbent assay. By using an array of purified schistosomal antigens, the patterns of antibody titers and isotypes differed for each experimental group and antigen. In the most-protective C57BL/6J sera, high levels of immunoglobulin G1 (IgG1), IgG2a, and IgG2b bound to heat shock protein 70 and the integral membrane protein Sm23, whereas recognition of these antigens by less-protective CBA/J sera was lower. Glutathione S-transferase (GST) was recognized predominantly by IgM antibodies of all vaccinated groups, and a significant portion of this response was directed against carbohydrate epitopes. Antibodies specific for triosephosphate isomerase, paramyosin, and Sm32 (hemoglobinase) were present in less-protective sera and thus seem less relevant for passive transfer of resistance. The results of this study suggest a contribution of IgG antibodies specific for heat shock protein 70 and Sm23, and possibly a contribution of GST-specific IgM antibodies, to the protective effect of sera from C57BL/6J mice vaccinated with irradiated cercariae.

References

  1. Anal Biochem. 1977 Oct;82(2):334-41 - PubMed
  2. Mem Inst Oswaldo Cruz. 1987;82 Suppl 4:115-9 - PubMed
  3. J Immunol. 1982 Apr;128(4):1880-4 - PubMed
  4. Exp Parasitol. 1982 Aug;54(1):21-32 - PubMed
  5. Am J Trop Med Hyg. 1967 Jul;16(4):483-6 - PubMed
  6. Parasite Immunol. 1990 Jan;12(1):15-32 - PubMed
  7. Nature. 1990 Aug 16;346(6285):623-8 - PubMed
  8. Parasite Immunol. 1990 Jul;12(4):341-52 - PubMed
  9. J Immunol. 1991 Mar 1;146(5):1655-60 - PubMed
  10. J Immunol. 1992 Jan 1;148(1):124-8 - PubMed
  11. Infect Immun. 1992 Jan;60(1):90-4 - PubMed
  12. Proc Natl Acad Sci U S A. 1992 Mar 1;89(5):1842-6 - PubMed
  13. J Immunol. 1992 Dec 15;149(12):3995-4001 - PubMed
  14. Infect Immun. 1993 Jan;61(1):146-54 - PubMed
  15. Parasitology. 1965 Nov;55(4):695-700 - PubMed
  16. Exp Parasitol. 1983 Feb;55(1):1-104 - PubMed
  17. Parasite Immunol. 1983 Nov;5(6):567-75 - PubMed
  18. J Immunol Methods. 1985 Apr 8;78(1):143-53 - PubMed
  19. Parasite Immunol. 1985 Mar;7(2):133-52 - PubMed
  20. Mol Cell Biol. 1985 Jun;5(6):1229-37 - PubMed
  21. Mol Biochem Parasitol. 1985 Sep;16(3):345-54 - PubMed
  22. J Immunol. 1986 Apr 1;136(7):2644-8 - PubMed
  23. J Immunol. 1986 Dec 1;137(11):3601-7 - PubMed
  24. J Immunol. 1987 May 15;138(10):3448-53 - PubMed
  25. J Immunol. 1987 Dec 1;139(11):3792-801 - PubMed
  26. Parasite Immunol. 1987 Sep;9(5):531-41 - PubMed
  27. Mol Biochem Parasitol. 1987 Oct;25(3):247-55 - PubMed
  28. Mol Biochem Parasitol. 1988 Jan 15;27(2-3):233-9 - PubMed
  29. J Immunol. 1988 May 1;140(9):3273-9 - PubMed
  30. Immunol Rev. 1988 Feb;102:5-28 - PubMed
  31. Proc Natl Acad Sci U S A. 1988 Aug;85(15):5678-82 - PubMed
  32. Parasitology. 1988 Oct;97 ( Pt 2):287-302 - PubMed
  33. J Parasitol. 1989 Apr;75(2):252-60 - PubMed
  34. Exp Parasitol. 1981 Aug;52(1):91-104 - PubMed

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