Display options
Cite
Thomas HM, Takeda J, Gould GW. Differential targeting of glucose transporter isoforms heterologously expressed in Xenopus oocytes. Biochem J. 1993;290:707-15doi: 10.1042/bj2900707.
Thomas, H. M., Takeda, J., & Gould, G. W. (1993). Differential targeting of glucose transporter isoforms heterologously expressed in Xenopus oocytes. The Biochemical journal, 290707-15. https://doi.org/10.1042/bj2900707
Thomas, H M, et al. "Differential targeting of glucose transporter isoforms heterologously expressed in Xenopus oocytes." The Biochemical journal vol. 290 (1993): 707-15. doi: https://doi.org/10.1042/bj2900707
Thomas HM, Takeda J, Gould GW. Differential targeting of glucose transporter isoforms heterologously expressed in Xenopus oocytes. Biochem J. 1993 Mar 15;290:707-15. doi: 10.1042/bj2900707. PMID: 8457198; PMCID: PMC1132338.
Copy Download .nbib Format: NLM
Share it on
Full text links
Silverchair Information Systems Free PMC Article

Biochem J. 1993 Mar 15;290:707-15. doi: 10.1042/bj2900707.

Differential targeting of glucose transporter isoforms heterologously expressed in Xenopus oocytes.

The Biochemical journal

H M Thomas, J Takeda, G W Gould

Affiliations

  1. Department of Biochemistry, University of Glasgow, Scotland, U.K.

PMID: 8457198 PMCID: PMC1132338 DOI: 10.1042/bj2900707
Free PMC Article

Abstract

We have examined the subcellular distribution of three members of the human glucose transporter family expressed in oocytes from Xenopus laevis. Following injection of in vitro-transcribed mRNA encoding the transporter isoform to be studied, we have determined the subcellular localization of the expressed protein by immunofluorescence and by subcellular fractionation coupled with immunoblotting using specific anti-peptide antibodies. We have shown that both the liver-type (GLUT 2) and brain-type (GLUT 3) glucose transporters are expressed predominantly in the plasma membranes of oocytes, and in both cases high levels of glucose transport activity are exhibited. In contrast, the insulin-regulatable glucose transporter (GLUT 4) is localized predominantly to an intracellular membrane pool, and the levels of transport activity recorded in oocytes expressing GLUT 4 are correspondingly lower. The localization of the different transporter isoforms to distinct subcellular fractions mirrors the situation observed in their native cell type and thus demonstrates that oocytes may prove to be a useful system with which to study the targeting signals for this important class of membrane proteins. In addition, the determination of the amounts of the transporters expressed per oocyte together with a knowledge of their Km values has allowed us to estimate the turnover numbers of these transporters. Insulin was without effect on glucose transport in oocytes expressing any of these transporter isoforms. Microinjection of guanosine 5'-[gamma-thio]triphosphate into oocytes expressing GLUT 4 was also without effect on the transport rate.

References

  1. J Biol Chem. 1992 Jul 25;267(21):14523-6 - PubMed
  2. J Cell Biol. 1991 Apr;113(1):123-35 - PubMed
  3. Diabetologia. 1992 Apr;35(4):304-9 - PubMed
  4. Diabetes. 1992 Oct;41(10):1360-5 - PubMed
  5. Biochem Biophys Res Commun. 1992 Oct 15;188(1):149-54 - PubMed
  6. Biochem Soc Trans. 1992 Aug;20(3):236S - PubMed
  7. Biochem J. 1993 Mar 15;290 ( Pt 3):701-6 - PubMed
  8. Nature. 1981 Nov 12;294(5837):175-6 - PubMed
  9. Science. 1985 Sep 6;229(4717):941-5 - PubMed
  10. Proc Natl Acad Sci U S A. 1986 Aug;83(16):5784-8 - PubMed
  11. J Clin Invest. 1987 Feb;79(2):657-61 - PubMed
  12. J Biol Chem. 1987 Aug 25;262(24):11817-24 - PubMed
  13. Methods Enzymol. 1987;152:276-84 - PubMed
  14. Methods Enzymol. 1987;152:288-96 - PubMed
  15. Diabetes. 1988 May;37(5):657-61 - PubMed
  16. Nature. 1988 May 12;333(6169):183-5 - PubMed
  17. Proc Natl Acad Sci U S A. 1988 Aug;85(15):5434-8 - PubMed
  18. Biochim Biophys Acta. 1988 Oct 7;971(3):339-50 - PubMed
  19. Cell. 1988 Oct 21;55(2):281-90 - PubMed
  20. J Biol Chem. 1988 Oct 25;263(30):15245-8 - PubMed
  21. J Biol Chem. 1989 Jan 15;264(2):856-61 - PubMed
  22. Nature. 1989 Mar 2;338(6210):83-7 - PubMed
  23. Cell. 1989 Apr 21;57(2):305-15 - PubMed
  24. Proc Natl Acad Sci U S A. 1989 Apr;86(8):2535-9 - PubMed
  25. Proc Natl Acad Sci U S A. 1989 Apr;86(8):2642-6 - PubMed
  26. Proc Natl Acad Sci U S A. 1989 May;86(9):3150-4 - PubMed
  27. J Biol Chem. 1989 May 15;264(14):7776-9 - PubMed
  28. Science. 1989 Jul 21;245(4915):295-7 - PubMed
  29. J Biol Chem. 1989 Nov 15;264(32):18884-9 - PubMed
  30. Proc Natl Acad Sci U S A. 1989 Nov;86(22):8688-92 - PubMed
  31. Am J Physiol. 1989 Nov;257(5 Pt 1):C851-8 - PubMed
  32. Biochemistry. 1989 Nov 28;28(24):9447-52 - PubMed
  33. Diabetes Care. 1990 Mar;13(3):198-208 - PubMed
  34. Trends Biochem Sci. 1990 Jan;15(1):18-23 - PubMed
  35. J Biol Chem. 1990 Aug 5;265(22):13276-82 - PubMed
  36. J Biol Chem. 1990 Aug 15;265(23):13801-8 - PubMed
  37. Diabetes. 1990 Jan;39(1):6-11 - PubMed
  38. J Biol Chem. 1990 Oct 25;265(30):18172-9 - PubMed
  39. Am J Physiol. 1990 Dec;259(6 Pt 1):C279-85 - PubMed
  40. Am J Physiol. 1990 Dec;259(6 Pt 1):C286-94 - PubMed
  41. J Biol Chem. 1991 Mar 5;266(7):4037-40 - PubMed
  42. Biochemistry. 1971 Mar 30;10(7):1148-53 - PubMed
  43. Biochemistry. 1973 Nov 6;12(23):4799-801 - PubMed
  44. Cell. 1979 Jul;17(3):517-26 - PubMed
  45. J Biol Chem. 1980 May 25;255(10):4758-62 - PubMed
  46. Eur J Biochem. 1981 Jan;113(2):339-48 - PubMed
  47. J Biol Chem. 1981 Apr 25;256(8):3685-9 - PubMed
  48. Biochem J. 1991 Apr 1;275 ( Pt 1):145-50 - PubMed
  49. Biochemistry. 1991 May 28;30(21):5139-45 - PubMed
  50. Proc Natl Acad Sci U S A. 1991 Sep 1;88(17):7815-9 - PubMed
  51. J Cell Biol. 1992 Feb;116(3):785-97 - PubMed
  52. Proc Natl Acad Sci U S A. 1992 Jan 15;89(2):688-92 - PubMed
  53. Proc Natl Acad Sci U S A. 1992 Jan 15;89(2):733-7 - PubMed
  54. Biochem Biophys Res Commun. 1992 Jan 31;182(2):703-11 - PubMed
  55. Biochem J. 1992 Feb 1;281 ( Pt 3):829-34 - PubMed
  56. J Biol Chem. 1992 Apr 15;267(11):7770-6 - PubMed
  57. J Cell Biol. 1992 May;117(4):729-43 - PubMed
  58. J Biol Chem. 1992 May 25;267(15):10393-9 - PubMed
  59. Proc Natl Acad Sci U S A. 1992 Aug 15;89(16):7556-60 - PubMed

Substances

MeSH terms

Publication Types

LinkOut - more resources