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Rudolphi U, Hohlbaum A, Lang B, et al. The B cell repertoire of patients with rheumatoid arthritis. Frequencies and specificities of peripheral blood B cells reacting with human IgG, human collagens, a mycobacterial heat shock protein and other antigens. Clin Exp Immunol. 1993;92(3):404-11doi: 10.1111/j.1365-2249.1993.tb03412.x.
Rudolphi, U., Hohlbaum, A., Lang, B., Peter, H. H., & Melchers, I. (1993). The B cell repertoire of patients with rheumatoid arthritis. Frequencies and specificities of peripheral blood B cells reacting with human IgG, human collagens, a mycobacterial heat shock protein and other antigens. Clinical and experimental immunology, 92(3), 404-11. https://doi.org/10.1111/j.1365-2249.1993.tb03412.x
Rudolphi, U, et al. "The B cell repertoire of patients with rheumatoid arthritis. Frequencies and specificities of peripheral blood B cells reacting with human IgG, human collagens, a mycobacterial heat shock protein and other antigens." Clinical and experimental immunology vol. 92,3 (1993): 404-11. doi: https://doi.org/10.1111/j.1365-2249.1993.tb03412.x
Rudolphi U, Hohlbaum A, Lang B, Peter HH, Melchers I. The B cell repertoire of patients with rheumatoid arthritis. Frequencies and specificities of peripheral blood B cells reacting with human IgG, human collagens, a mycobacterial heat shock protein and other antigens. Clin Exp Immunol. 1993 Jun;92(3):404-11. doi: 10.1111/j.1365-2249.1993.tb03412.x. PMID: 8099856; PMCID: PMC1554766.
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Clin Exp Immunol. 1993 Jun;92(3):404-11. doi: 10.1111/j.1365-2249.1993.tb03412.x.

The B cell repertoire of patients with rheumatoid arthritis. Frequencies and specificities of peripheral blood B cells reacting with human IgG, human collagens, a mycobacterial heat shock protein and other antigens.

Clinical and experimental immunology

U Rudolphi, A Hohlbaum, B Lang, H H Peter, I Melchers

Affiliations

  1. Clinical Research Unit, University of Freiburg, Germany.

PMID: 8099856 PMCID: PMC1554766 DOI: 10.1111/j.1365-2249.1993.tb03412.x
Free PMC Article

Abstract

Using a potent in vitro limiting dilution culture system, we have activated human peripheral blood B cells to proliferate and to differentiate into antibody-secreting cells (ASC). Under these conditions 25-100% of B cells are clonally expanded and produce IgM, IgG or IgA. Culture supernatants were tested for antibodies binding to human IgG-Fc fragments (RF), the 65-kD heat shock protein of Mycobacterium bovis (hsp60), human collagens type I, II, IV, V, transferrin, lactoferrin, albumins, and gelatine. All blood samples contained precursors of ASC (p-ASC) able to produce IgM binding to these antigens in frequencies above 0.03% of B cells. Most interestingly, a significant difference exists between rheumatoid arthritis (RA) patients and controls, concerning the relative frequencies of p-ASC able to produce monospecific or multireactive RF. Whereas most p-ASC(RF) in RA patients are monospecific (mean ratio 3.7), most p-ASC(RF) in healthy control persons are cross-reactive with at least one of five other antigens tested (mean ratio 0.2). The data suggest a disease-specific expansion of p-ASC committed to the production of monospecific rheumatoid factors.

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References

  1. APMIS. 1990 May;98(5):383-94 - PubMed
  2. Science. 1989 Sep 8;245(4922):1112-5 - PubMed
  3. Eur J Immunol. 1991 Nov;21(11):2749-54 - PubMed
  4. J Clin Invest. 1990 Oct;86(4):1320-8 - PubMed
  5. Arthritis Rheum. 1989 Feb;32(2):134-8 - PubMed
  6. N Engl J Med. 1978 Apr 20;298(16):869-71 - PubMed
  7. Scand J Rheumatol Suppl. 1988;75:123-6 - PubMed
  8. Eur J Immunol. 1992 Jan;22(1):51-6 - PubMed
  9. Ann Rheum Dis. 1989 Feb;48(2):118-23 - PubMed
  10. Scand J Immunol. 1990 Jun;31(6):283-8 - PubMed
  11. Int Rev Immunol. 1988 Mar;3(1-2):47-58 - PubMed
  12. J Immunol. 1990 May 15;144(10):3741-7 - PubMed
  13. J Clin Invest. 1991 Jul;88(1):193-203 - PubMed
  14. J Immunol. 1988 Dec 15;141(12):4165-72 - PubMed
  15. Eur J Immunol. 1987 Jun;17(6):887-92 - PubMed
  16. J Exp Med. 1982 Dec 1;156(6):1587-603 - PubMed
  17. Arthritis Rheum. 1981 Nov;24(11):1370-6 - PubMed
  18. J Immunol. 1987 Mar 1;138(5):1391-6 - PubMed
  19. Immunol Rev. 1990 Dec;118:285-310 - PubMed
  20. Clin Exp Immunol. 1988 Nov;74(2):211-5 - PubMed
  21. Annu Rev Immunol. 1991;9:567-89 - PubMed
  22. Annu Rev Immunol. 1984;2:199-218 - PubMed
  23. Arthritis Rheum. 1989 Sep;32(9):1087-92 - PubMed
  24. J Exp Med. 1988 Dec 1;168(6):1979-92 - PubMed
  25. J Immunol. 1990 Sep 15;145(6):1681-6 - PubMed
  26. Eur J Immunol. 1985 Feb;15(2):124-31 - PubMed
  27. EMBO J. 1991 Dec;10(12):3641-5 - PubMed
  28. J Immunol. 1989 Apr 15;142(8):2778-83 - PubMed
  29. Curr Biol. 1991 Oct;1(5):315-7 - PubMed
  30. J Clin Invest. 1991 Feb;87(2):379-83 - PubMed
  31. Clin Exp Immunol. 1989 Oct;78(1):13-8 - PubMed
  32. Proc Natl Acad Sci U S A. 1990 Oct;87(20):7907-11 - PubMed
  33. J Immunol. 1981 Apr;126(4):1614-9 - PubMed
  34. Immunol Today. 1991 May;12(5):154-9 - PubMed
  35. Arthritis Rheum. 1982 Dec;25(12):1445-50 - PubMed
  36. Scand J Immunol. 1990 Oct;32(4):347-57 - PubMed

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