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Györke S, Vélez P, Suárez-Isla B, et al. Activation of single cardiac and skeletal ryanodine receptor channels by flash photolysis of caged Ca2+. Biophys J. 1994;66(6):1879-86doi: 10.1016/S0006-3495(94)80981-6.
Györke, S., Vélez, P., Suárez-Isla, B., & Fill, M. (1994). Activation of single cardiac and skeletal ryanodine receptor channels by flash photolysis of caged Ca2+. Biophysical journal, 66(6), 1879-86. https://doi.org/10.1016/S0006-3495(94)80981-6
Györke, S, et al. "Activation of single cardiac and skeletal ryanodine receptor channels by flash photolysis of caged Ca2+." Biophysical journal vol. 66,6 (1994): 1879-86. doi: https://doi.org/10.1016/S0006-3495(94)80981-6
Györke S, Vélez P, Suárez-Isla B, Fill M. Activation of single cardiac and skeletal ryanodine receptor channels by flash photolysis of caged Ca2+. Biophys J. 1994 Jun;66(6):1879-86. doi: 10.1016/S0006-3495(94)80981-6. PMID: 8075325; PMCID: PMC1275913.
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Elsevier Science Free PMC Article

Biophys J. 1994 Jun;66(6):1879-86. doi: 10.1016/S0006-3495(94)80981-6.

Activation of single cardiac and skeletal ryanodine receptor channels by flash photolysis of caged Ca2+.

Biophysical journal

S Györke, P Vélez, B Suárez-Isla, M Fill

Affiliations

  1. Department of Physiology and Biophysics, University of Texas Medical Branch, Galveston 77555-0641.

PMID: 8075325 PMCID: PMC1275913 DOI: 10.1016/S0006-3495(94)80981-6
Free PMC Article

Abstract

Single ryanodine-sensitive sarcoplasmic reticulum (SR) Ca2+ release channels isolated from rabbit skeletal and canine cardiac muscle were reconstituted in planar lipid bilayers. Single channel activity was measured in simple solutions (no ATP or Mg2+) with 250 mM symmetrical Cs+ as charge carrier. A laser flash was used to photolyze caged-Ca2+ (DM-nitrophen) in a small volume directly in front of the bilayer. The free [Ca2+] in this small volume and in the bulk solution was monitored with Ca2+ electrodes. This setup allowed fast, calibrated free [Ca2+] stimuli to be applied repetitively to single SR Ca2+ release channels. A standard photolytically induced free [Ca2+] step (pCa 7-->6) was applied to both the cardiac and skeletal release channels. The rate of channel activation was determined by fitting a single exponential to ensemble currents generated from at least 50 single channel sweeps. The time constants of activation were 1.43 +/- 0.65 ms (mean +/- SD; n = 5) and 1.28 +/- 0.61 ms (n = 5) for cardiac and skeletal channels, respectively. This study presents a method for defining the fast Ca2+ regulation kinetics of single SR Ca2+ release channels and shows that the activation rate of skeletal SR Ca2+ release channels is consistent with a role for CICR in skeletal muscle excitation-contraction coupling.

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