Display options
Share it on

Inflammation. 1994 Apr;18(2):151-61. doi: 10.1007/BF01534556.

Decreased production of nitric oxide by human neutrophils during septic multiple organ dysfunction syndrome. Comparison with endotoxin and cytokine effects on normal cells.

Inflammation

M C Carreras, S D Catz, G A Pargament, C G Del Bosco, J J Poderoso

Affiliations

  1. Laboratory of Oxygen Metabolism, University Hospital School of Medicine, University of Buenos Aires, Argentina.

PMID: 8070900 DOI: 10.1007/BF01534556

Abstract

The objective of this study was to determine nitric oxide (NO) and superoxide anion release (O-2) by neutrophils (PMNs) in the septic multiple organ dysfunction syndrome (MODS) and to compare them with the response of normal cells to lipopolysaccharide (LPS) and cytokines. NO production was measured by the release of nitrites in the medium, its maximal production rate by a modified oxyhemoglobin assay and O-2 by standard methods. Normal cells were incubated with LPS, gamma interferon (IFN-gamma), or tumor necrosis factor (TNF-alpha) alone or in combination. Results showed that PMN release of both NO and O-2 was reduced in septic samples; in contrast, an association of LPS, IFN-gamma, and TNF-alpha promoted maximal NO release by normal cells (40-50%). We conclude that while interaction of normal PMNs with cytokines increases NO and O-2 release, progression of sepsis to a multiple organ dysfunction impairs these responses in both functions.

References

  1. J Biol Chem. 1991 Feb 25;266(6):3369-71 - PubMed
  2. J Leukoc Biol. 1991 Jul;50(1):93-103 - PubMed
  3. Lancet. 1991 Sep 21;338(8769):732-6 - PubMed
  4. Biochem J. 1989 Jul 1;261(1):293-6 - PubMed
  5. J Leukoc Biol. 1991 Aug;50(2):131-9 - PubMed
  6. Crit Care Med. 1989 Dec;17(12):1241-6 - PubMed
  7. Biochem Biophys Res Commun. 1991 Jun 14;177(2):828-33 - PubMed
  8. Immunology. 1990 May;70(1):82-7 - PubMed
  9. FEBS Lett. 1989 Feb 27;244(2):357-60 - PubMed
  10. Methods Enzymol. 1984;105:358-65 - PubMed
  11. Crit Care Med. 1992 Jan;20(1):11-6 - PubMed
  12. Proc Natl Acad Sci U S A. 1990 Dec;87(24):10043-7 - PubMed
  13. Eur J Immunol. 1991 Oct;21(10):2523-7 - PubMed
  14. J Immunol. 1988 Oct 1;141(7):2407-12 - PubMed
  15. Blood. 1989 Nov 1;74(6):1885-7 - PubMed
  16. Biochem Biophys Res Commun. 1991 Aug 15;178(3):884-91 - PubMed
  17. FEBS Lett. 1990 Nov 26;275(1-2):87-90 - PubMed
  18. Eicosanoids. 1990;3(4):243-5 - PubMed
  19. Arch Surg. 1988 Jan;123(1):17-22 - PubMed
  20. Lancet. 1989 May 20;1(8647):1122-6 - PubMed
  21. Immunology. 1990 Dec;71(4):556-9 - PubMed
  22. Proc Natl Acad Sci U S A. 1990 Sep;87(17):6758-61 - PubMed
  23. Eur J Clin Invest. 1991 Aug;21(4):361-74 - PubMed
  24. Proc Natl Acad Sci U S A. 1989 Aug;86(16):6328-32 - PubMed
  25. Arch Surg. 1988 Feb;123(2):171-5 - PubMed
  26. Biochem Biophys Res Commun. 1990 Dec 14;173(2):541-7 - PubMed
  27. Lancet. 1991 Dec 21-28;338(8782-8783):1557-8 - PubMed
  28. Lancet. 1991 Dec 21-28;338(8782-8783):1555-7 - PubMed
  29. Pharmacol Rev. 1991 Jun;43(2):109-42 - PubMed
  30. J Clin Invest. 1990 Jul;86(1):184-95 - PubMed
  31. Chest. 1992 Jun;101(6):1644-55 - PubMed
  32. Nature. 1990 May 3;345(6270):27-8 - PubMed
  33. J Leukoc Biol. 1991 May;49(5):449-54 - PubMed
  34. Am J Physiol. 1990 Jul;259(1 Pt 2):R38-44 - PubMed
  35. J Immunol. 1986 Feb 1;136(3):860-6 - PubMed
  36. Curr Opin Immunol. 1991 Feb;3(1):65-70 - PubMed

Substances

MeSH terms

Publication Types