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Langley KE, Bennett LG, Wypych J, et al. Soluble stem cell factor in human serum. Blood. 1993;81(3):656-60
Langley, K. E., Bennett, L. G., Wypych, J., Yancik, S. A., Liu, X. D., Westcott, K. R., Chang, D. G., Smith, K. A., & Zsebo, K. M. (1993). Soluble stem cell factor in human serum. Blood, 81(3), 656-60.
Langley, K E, et al. "Soluble stem cell factor in human serum." Blood vol. 81,3 (1993): 656-60.
Langley KE, Bennett LG, Wypych J, Yancik SA, Liu XD, Westcott KR, Chang DG, Smith KA, Zsebo KM. Soluble stem cell factor in human serum. Blood. 1993 Feb 01;81(3):656-60. PMID: 7678995.
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Blood. 1993 Feb 01;81(3):656-60.

Soluble stem cell factor in human serum.

Blood

K E Langley, L G Bennett, J Wypych, S A Yancik, X D Liu, K R Westcott, D G Chang, K A Smith, K M Zsebo

Affiliations

  1. Amgen, Inc., Thousand Oaks, CA 91320.

PMID: 7678995

Abstract

Stem cell factor (SCF) is a recently described factor active in the early stages of hematopoiesis. It can exist in membrane-bound form and in proteolytically released soluble form. The levels and nature of SCF in human serum are described. As determined by an enzyme-linked immunosorbent assay performed for 257 samples, SCF level in serum averaged 3.3 +/- 1.1 ng/mL. The serum SCF was partially purified by immunoaffinity chromatography and analyzed by glycosidase treatments in conjunction with sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. The results show that the SCF has N-linked and O-linked carbohydrate and corresponds to the soluble form, at or about 165 amino acids in length. The findings suggest functional importance for soluble SCF in humans.

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