Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1995;13(2):138-41.

[Exploration on experimental conditions of dot-ELISA for Trichomonas vaginalis using intact parasite antigen-monoclonal antibody detecting system].

Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases

[Article in Chinese]
C Yu, X Gao, Y Zhu

PMID: 7554165

Abstract

This paper deals with the effect of different experimental conditions including the number of parasite, blocking fluid, incubation time and different types of antigens on dot-ELISA for Trichomonas vaginalis. Five microlitres of freshly washed intact worms at a concentration of 7.7 x 10(5)-3 x 10(8) cells/ml were added onto nitrocellulose membrane blocked with 1% BSA, 10% bovine serum or milk power (0.1%, 0.25%, 0.5%, 5%). Dot-ELISA using nitrocellulose membrane containing whole cell antigen 2.5 x 10(7) cells/ml, blocked by 0.5% defatted milk power at 37 degrees C for one hour before the incubation in McAb, incubated at 37 degrees C for one hour in McAb and SPA respectively was found to give more promising results. The whole cell antigen could be preserved for four months at 4 degrees C and -20 degrees C.

Substances

• Antibodies, Monoclonal
• Antibodies, Protozoan
• Antigens, Protozoan

MeSH terms

• Animals
• Antibodies, Monoclonal
• Antibodies, Protozoan / analysis
• Antigens, Protozoan / immunology
• Cattle
• Enzyme-Linked Immunosorbent Assay / methods
• Trichomonas vaginalis / immunology

Publication Types

• Comparative Study
• English Abstract
• Journal Article