Display options
Cite
Vuorinen KH, Peuhkurinen KJ, Kiviluoma KT, et al. Efflux of adenosine and total adenylate catabolites during alterations of the cellular energy state. An NMR study of continuous and discontinuous ischemia. Basic Res Cardiol. 1995;90(3):211-9doi: 10.1007/BF00805664.
Vuorinen, K. H., Peuhkurinen, K. J., Kiviluoma, K. T., & Hassinen, I. E. (1995). Efflux of adenosine and total adenylate catabolites during alterations of the cellular energy state. An NMR study of continuous and discontinuous ischemia. Basic research in cardiology, 90(3), 211-9. https://doi.org/10.1007/BF00805664
Vuorinen, K H, et al. "Efflux of adenosine and total adenylate catabolites during alterations of the cellular energy state. An NMR study of continuous and discontinuous ischemia." Basic research in cardiology vol. 90,3 (1995): 211-9. doi: https://doi.org/10.1007/BF00805664
Vuorinen KH, Peuhkurinen KJ, Kiviluoma KT, Hassinen IE. Efflux of adenosine and total adenylate catabolites during alterations of the cellular energy state. An NMR study of continuous and discontinuous ischemia. Basic Res Cardiol. 1995 May-Jun;90(3):211-9. doi: 10.1007/BF00805664. PMID: 7575374.
Copy Download .nbib Format: NLM
Share it on

Basic Res Cardiol. 1995 May-Jun;90(3):211-9. doi: 10.1007/BF00805664.

Efflux of adenosine and total adenylate catabolites during alterations of the cellular energy state. An NMR study of continuous and discontinuous ischemia.

Basic research in cardiology

K H Vuorinen, K J Peuhkurinen, K T Kiviluoma, I E Hassinen

Affiliations

  1. Department of Medical Biochemistry, University of Oulu, Finland.

PMID: 7575374 DOI: 10.1007/BF00805664

Abstract

Reperfusion after continuous or discontinuous ischemia has a bearing on clinical interventions. An important question is the washout of metabolites after periods of diminished energy state of the myocardial cell. We therefore set out to determine the washout of adenosine and its metabolites after periods of ischemia in an experimental set-up which allowed non-destructive monitoring of the cellular energy state and cytosolic pH over consecutive time intervals. Isolated rat hearts were perfused with hemoglobin-free saline in a nuclear magnetic resonance spectrometer equipped for 31P NMR spectroscopy of phosphorus-containing metabolites, which could be measured over 3-min time blocks. The response of the heart when subjected to 18 min of continuous ischemia and subsequent reperfusion was compared with that when subjected to three 6-min periods of ischemia separated by 3-min periods of reperfusion. The mechanical performance of the hearts, oxygen consumption and efflux of adenosine and its metabolites were measured. The consecutive ischemic periods produced no evidence of preconditioning as judged from the cellular energy state, although the mechanical recovery was better than after continuous ischemia. During the repetitive ischemia/reperfusion protocol the efflux of adenosine was smaller, although the efflux of combined adenylate catabolites did not differ from that after continuous ischemia. The results do not support the view of adenosine being a major effector in the phenomenon of preconditioning.

References

  1. Circ Res. 1980 Dec;47(6):807-13 - PubMed
  2. Basic Res Cardiol. 1988 Jul-Aug;83(4):431-44 - PubMed
  3. Eur J Biochem. 1986 Aug 15;159(1):203-13 - PubMed
  4. J Lab Clin Med. 1987 Jul;110(1):13-30 - PubMed
  5. Biochem J. 1991 Dec 15;280 ( Pt 3):561-73 - PubMed
  6. Biochemistry. 1989 Jun 27;28(13):5323-6 - PubMed
  7. Circulation. 1994 May;89(5):2283-9 - PubMed
  8. Circulation. 1989 Oct;80(4):1049-62 - PubMed
  9. Biochem Biophys Res Commun. 1975 Jul 22;65(2):575-82 - PubMed
  10. Physiol Rev. 1988 Jan;68(1):232-307 - PubMed
  11. Circulation. 1991 Nov;84(5 Suppl):III369-74 - PubMed
  12. Biochim Biophys Acta. 1970 Jan 14;198(1):101-12 - PubMed
  13. Circulation. 1991 Dec;84(6):2495-503 - PubMed
  14. Circ Res. 1992 Sep;71(3):590-604 - PubMed
  15. Am J Physiol. 1988 Jul;255(1 Pt 2):H189-96 - PubMed
  16. Circulation. 1990 Sep;82(3):723-38 - PubMed
  17. Cardiology. 1971;56(1):22-34 - PubMed
  18. J Mol Cell Cardiol. 1988 Oct;20(10):887-96 - PubMed
  19. Circ Res. 1976 May;38(5 Suppl 1):I52-74 - PubMed
  20. Am J Physiol. 1986 Mar;250(3 Pt 2):H503-8 - PubMed
  21. Circ Res. 1973 Jun;32(6):699-711 - PubMed
  22. J Clin Invest. 1985 Nov;76(5):1713-9 - PubMed
  23. Clin Res. 1987 Jan;35(1):13-20 - PubMed
  24. Circ Res. 1990 Apr;66(4):913-31 - PubMed
  25. Circulation. 1992 Feb;85(2):659-65 - PubMed
  26. J Biol Chem. 1983 Aug 25;258(16):9657-61 - PubMed
  27. Acta Physiol Scand Suppl. 1987;559:1-85 - PubMed
  28. Am J Physiol. 1963 Feb;204:317-22 - PubMed
  29. Circ Res. 1984 Nov;55(5):689-701 - PubMed
  30. Circulation. 1993 Jan;87(1):208-15 - PubMed
  31. Circulation. 1991 Jul;84(1):442-5 - PubMed
  32. Biochim Biophys Acta. 1984 Jun 19;804(2):161-8 - PubMed
  33. Circulation. 1991 Jul;84(1):350-6 - PubMed
  34. Prog Cardiovasc Dis. 1989 Jul-Aug;32(1):73-97 - PubMed
  35. J Mol Cell Cardiol. 1986 Nov;18(11):1133-42 - PubMed
  36. Biochem J. 1986 May 1;235(3):847-51 - PubMed
  37. Cardiovasc Res. 1992 May;26(5):502-7 - PubMed
  38. Circulation. 1991 Dec;84(6):2504-12 - PubMed
  39. Circulation. 1986 Nov;74(5):1124-36 - PubMed
  40. Biochim Biophys Acta. 1990 Aug 9;1019(1):73-80 - PubMed
  41. Am J Physiol. 1987 Mar;252(3 Pt 2):H566-77 - PubMed
  42. Basic Res Cardiol. 1981 Jan-Feb;76(1):44-61 - PubMed

Substances

MeSH terms

Publication Types