Virchows Arch. 1995;426(4):393-9. doi: 10.1007/BF00191349.

Contraction band necrosis at the lateral borders of the area at risk in reperfused infarcts. Observations in a pig model of in situ coronary occlusion.

Virchows Archiv : an international journal of pathology

J Solares, D Garcia-Dorado, J Oliveras, M A González, M Ruiz-Meana, J A Barrabés, C Gonzalez-Bravo, J Soler-Soler

PMID: 7599792 DOI: 10.1007/BF00191349

Abstract

The aim of this study was to test the hypothesis that increased mechanical stress at the lateral borders of the area at risk may render this area more susceptible to ischaemia/reperfusion injury in the absence of collateral flow. The spatial distribution of myocardial necrosis within the territory of a transiently occluded left anterior descending coronary artery was investigated in 31 porcine hearts submitted to 48 min of coronary occlusion and 6 h of reperfusion. Immediately before excising the heart, the left anterior descending coronary artery was re-occluded and 10% fluorescein was injected in the left atrium. The area at risk was imaged by ultraviolet illumination of the myocardial slices, and the area of necrosis by incubation in triphenyltetrazolium chloride. The area at risk was divided in four sectors and an index of eccentricity was calculated as the percent of the area of necrosis located in the two lateral sectors of the area at risk. The area of contraction band necrosis was measured in whole heart histological sections. Infarcts were generally small, and were composed almost exclusively of contraction band necrosis. There was a good correlation between the extent of the area of contraction band necrosis and infarct size (r = 0.831, P < 0.0005). The area of necrosis had a patchy appearance and was predominantly distributed along the lateral borders of the area at risk. This eccentric distribution was more prominent in smaller infarcts, and the eccentricity index was inversely correlated with infarct size (r = -0.471, P = 0.007), suggesting that contraction band necrosis occurs first at the interface between control and reperfused myocardium in this model. These results are in agreement with a prominent role of mechanical factors in the genesis of myocardial necrosis during transient coronary occlusion.

References

1. Circulation. 1977 Feb;55(2):279-85 - PubMed
2. J Cardiovasc Pharmacol. 1993 May;21(5):693-700 - PubMed
3. Circulation. 1989 Dec;80(6):1816-27 - PubMed
4. Circ Res. 1985 Dec;57(6):864-75 - PubMed
5. Am J Physiol. 1991 Feb;260(2 Pt 2):H426-35 - PubMed
6. J Mol Cell Cardiol. 1973 Aug;5(4):395-407 - PubMed
7. J Am Coll Cardiol. 1993 Sep;22(3):720-6 - PubMed
8. Circ Res. 1987 Apr;60(4):478-86 - PubMed
9. Chest. 1978 Jun;73(6):843-9 - PubMed
10. Circ Res. 1982 Dec;51(6):683-93 - PubMed
11. Am J Physiol. 1986 Feb;250(2 Pt 2):H265-75 - PubMed
12. Am J Physiol. 1989 May;256(5 Pt 2):H1266-73 - PubMed
13. Am Heart J. 1993 Jun;125(6):1553-63 - PubMed
14. Cardiovasc Res. 1993 Aug;27(8):1462-9 - PubMed
15. Am J Physiol. 1990 Feb;258(2 Pt 2):H285-91 - PubMed
16. Lab Invest. 1979 Jun;40(6):633-44 - PubMed
17. Circulation. 1989 Jul;80(1):165-77 - PubMed
18. Cardiovasc Res. 1993 Aug;27(8):1387-403 - PubMed
19. Am J Physiol. 1992 Dec;263(6 Pt 2):H1732-8 - PubMed
20. Circulation. 1987 May;75(5):1074-82 - PubMed
21. Am J Cardiol. 1961 Dec;8:792-8 - PubMed
22. Am J Pathol. 1990 Aug;137(2):301-11 - PubMed
23. Hum Pathol. 1990 May;21(5):530-5 - PubMed
24. Circulation. 1992 Mar;85(3):1160-74 - PubMed
25. Am J Physiol. 1982 Feb;242(2):H240-4 - PubMed

MeSH terms

• Animals
• Coronary Disease / physiopathology
• Image Processing, Computer-Assisted
• Myocardial Contraction
• Myocardial Reperfusion Injury / pathology
• Stress, Mechanical
• Swine

Publication Types

• Journal Article
• Research Support, Non-U.S. Gov't