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Eur J Clin Pharmacol. 1980 Oct;18(4):339-45. doi: 10.1007/BF00561392.

The radioimmunoassay of buprenorphine.

European journal of clinical pharmacology

A J Bartlett, J G Lloyd-Jones, M J Rance, I R Flockhart, G J Dockray, M R Bennett, R A Moore

PMID: 7439255 DOI: 10.1007/BF00561392


Antisera to buprenorphine were obtained in rabbits immunised with 3-0-carboxymethylbuprenorphine and N-hemisuccinyl-norbuprenorphine conjugated to bovine serum albumin. Using the latter antiserum and tritium labelled buprenorphine a radioimmunoassay have good accuracy and precision was developed for concentrations as low as 50 picograms in 1 ml of plasma. The N-hemisuccinyl antiserum crossreacted with norbuprenorphine, and the 3-0-glucuronide conjugate with the 3-0-carboxymethyl antiserum. Cross-reactivity of both antisera to other pharmacologically related compounds was negligible. The assay was employed to determine plasma buprenorphine concentration following its parenteral administration to dog and man.

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