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Williams SK, Howarth NL, Devenny JJ, et al. Structural and functional consequences of increased tubulin glycosylation in diabetes mellitus. Proc Natl Acad Sci U S A. 1982;79(21):6546-50doi: 10.1073/pnas.79.21.6546.
Williams, S. K., Howarth, N. L., Devenny, J. J., & Bitensky, M. W. (1982). Structural and functional consequences of increased tubulin glycosylation in diabetes mellitus. Proceedings of the National Academy of Sciences of the United States of America, 79(21), 6546-50. https://doi.org/10.1073/pnas.79.21.6546
Williams, S K, et al. "Structural and functional consequences of increased tubulin glycosylation in diabetes mellitus." Proceedings of the National Academy of Sciences of the United States of America vol. 79,21 (1982): 6546-50. doi: https://doi.org/10.1073/pnas.79.21.6546
Williams SK, Howarth NL, Devenny JJ, Bitensky MW. Structural and functional consequences of increased tubulin glycosylation in diabetes mellitus. Proc Natl Acad Sci U S A. 1982 Nov;79(21):6546-50. doi: 10.1073/pnas.79.21.6546. PMID: 6959136; PMCID: PMC347164.
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Proc Natl Acad Sci U S A. 1982 Nov;79(21):6546-50. doi: 10.1073/pnas.79.21.6546.

Structural and functional consequences of increased tubulin glycosylation in diabetes mellitus.

Proceedings of the National Academy of Sciences of the United States of America

S K Williams, N L Howarth, J J Devenny, M W Bitensky

PMID: 6959136 PMCID: PMC347164 DOI: 10.1073/pnas.79.21.6546
Free PMC Article

Abstract

The extent of in vitro nonenzymatic glycosylation of purified rat brain tubulin was dependent on time and glucose concentration. Tubulin glycosylation profoundly inhibited GTP-dependent tubulin polymerization. Electron microscopy and NaDodSO4/polyacrylamide gel electrophoresis showed that glycosylated tubulin forms high molecular weight amorphous aggregates that are not disrupted by detergents or reducing agents. The amount of covalently bound NaB3H4-reducible sugars in tubulin recovered from brain of streptozotocin-induced diabetic rats was dramatically increased as compared with tubulin recovered from normal rat brain. Moreover, tubulin recovered from diabetic rat brain exhibited less GTP-induced polymerization than tubulin from nondiabetic controls. The possible implications of these data for diabetic neuropathy are discussed.

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References

  1. J Ultrastruct Res. 1969 Jan;26(1):31-43 - PubMed
  2. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
  3. J Cell Biol. 1971 Oct;51(1):138-47 - PubMed
  4. Clin Endocrinol Metab. 1972 Nov;1(3):809-27 - PubMed
  5. Fed Proc. 1974 Feb;33(2):167-74 - PubMed
  6. Ann N Y Acad Sci. 1975 Jun 30;253:472-506 - PubMed
  7. Ann N Y Acad Sci. 1975 Jun 30;253:517-27 - PubMed
  8. Ann N Y Acad Sci. 1975 Jun 30;253:535-44 - PubMed
  9. Diabetes. 1975 Dec;24(12):1081-5 - PubMed
  10. Biochim Biophys Acta. 1976 Apr 13;457(1):57-108 - PubMed
  11. Acta Physiol Scand. 1976 Apr;96(4):478-85 - PubMed
  12. Diabetes. 1977 May;26(5):510-7 - PubMed
  13. J Biol Chem. 1977 May 10;252(9):2998-3002 - PubMed
  14. Biochemistry. 1977 Dec 13;16(25):5610-7 - PubMed
  15. Metabolism. 1978 Feb;27(2):235-52 - PubMed
  16. Science. 1978 Apr 7;200(4337):21-7 - PubMed
  17. Med Clin North Am. 1978 Jul;62(4):747-52 - PubMed
  18. J Biol Chem. 1979 Feb 10;254(3):595-7 - PubMed
  19. Ann Intern Med. 1979 Mar;90(3):298-303 - PubMed
  20. J Exp Med. 1979 Nov 1;150(5):1098-107 - PubMed
  21. Biochem Biophys Res Commun. 1979 Nov 28;91(2):498-501 - PubMed
  22. Tohoku J Exp Med. 1979 Dec;129(4):357-66 - PubMed
  23. J Clin Invest. 1980 Apr;65(4):896-901 - PubMed
  24. J Biol Chem. 1980 Jul 25;255(14):6717-20 - PubMed
  25. Diabetes. 1980 Nov;29(11):931-42 - PubMed
  26. FEBS Lett. 1980 Oct 20;120(1):1-3 - PubMed
  27. Proc Natl Acad Sci U S A. 1981 Apr;78(4):2393-7 - PubMed
  28. Neurology. 1961 Apr;11(4)Pt 1:275-84 - PubMed

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