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Rose K, Simona MG, Offord RE. Amino acid sequence determination by g.l.c.--mass spectrometry of permethylated peptides. Optimization of the formation of chemical derivatives at the 2-10 nmol level. Biochem J. 1983;215(2):261-72doi: 10.1042/bj2150261.
Rose, K., Simona, M. G., & Offord, R. E. (1983). Amino acid sequence determination by g.l.c.--mass spectrometry of permethylated peptides. Optimization of the formation of chemical derivatives at the 2-10 nmol level. The Biochemical journal, 215(2), 261-72. https://doi.org/10.1042/bj2150261
Rose, K, et al. "Amino acid sequence determination by g.l.c.--mass spectrometry of permethylated peptides. Optimization of the formation of chemical derivatives at the 2-10 nmol level." The Biochemical journal vol. 215,2 (1983): 261-72. doi: https://doi.org/10.1042/bj2150261
Rose K, Simona MG, Offord RE. Amino acid sequence determination by g.l.c.--mass spectrometry of permethylated peptides. Optimization of the formation of chemical derivatives at the 2-10 nmol level. Biochem J. 1983 Nov 01;215(2):261-72. doi: 10.1042/bj2150261. PMID: 6418140; PMCID: PMC1152393.
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Biochem J. 1983 Nov 01;215(2):261-72. doi: 10.1042/bj2150261.

Amino acid sequence determination by g.l.c.--mass spectrometry of permethylated peptides. Optimization of the formation of chemical derivatives at the 2-10 nmol level.

The Biochemical journal

K Rose, M G Simona, R E Offord

PMID: 6418140 PMCID: PMC1152393 DOI: 10.1042/bj2150261
Free PMC Article

Abstract

A new technique is described that permits the permethylation of acylated peptides at the 2-10 nmol level. The presence of up to 400 micrograms of sodium dodecyl sulphate per sample does not affect the reaction yields. The technique, which is a miniaturization of the widely used methyl iodide/dimethylsulphinyl carbanion procedure, employs a layer of hexane to exclude moisture and oxygen from the reaction mixture. Analysis of the peptide derivatives by combined g.l.c.--mass spectrometry permits amino acid sequence information to be obtained. In addition to studies of digests of a model substrate (glucagon), the new permethylation technique has been used to identify a peptide of interest from a digest of a cytochrome and to define the N-termini of two proteins at the 5 nmol level.

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