Display options
Cite
Deuring R, Klotz G, Doerfler W. An unusual symmetric recombinant between adenovirus type 12 DNA and human cell DNA. Proc Natl Acad Sci U S A. 1981;78(5):3142-6doi: 10.1073/pnas.78.5.3142.
Deuring, R., Klotz, G., & Doerfler, W. (1981). An unusual symmetric recombinant between adenovirus type 12 DNA and human cell DNA. Proceedings of the National Academy of Sciences of the United States of America, 78(5), 3142-6. https://doi.org/10.1073/pnas.78.5.3142
Deuring, R, et al. "An unusual symmetric recombinant between adenovirus type 12 DNA and human cell DNA." Proceedings of the National Academy of Sciences of the United States of America vol. 78,5 (1981): 3142-6. doi: https://doi.org/10.1073/pnas.78.5.3142
Deuring R, Klotz G, Doerfler W. An unusual symmetric recombinant between adenovirus type 12 DNA and human cell DNA. Proc Natl Acad Sci U S A. 1981 May;78(5):3142-6. doi: 10.1073/pnas.78.5.3142. PMID: 6265937; PMCID: PMC319516.
Copy Download .nbib Format: NLM
Share it on
Full text links
Atypon Free PMC Article

Proc Natl Acad Sci U S A. 1981 May;78(5):3142-6. doi: 10.1073/pnas.78.5.3142.

An unusual symmetric recombinant between adenovirus type 12 DNA and human cell DNA.

Proceedings of the National Academy of Sciences of the United States of America

R Deuring, G Klotz, W Doerfler

PMID: 6265937 PMCID: PMC319516 DOI: 10.1073/pnas.78.5.3142
Free PMC Article

Abstract

On purification of human adenovirus type 12 (Ad12) by equilibrium sedimentation in CsCl density gradients, two bands of particles, Ad12-3 and Ad12-3a, are observed. The particles from band Ad12-3a contain a recombinant of human host cell DNA and of Ad12 DNA. The human cell DNA sequences contain repetitive DNA recurring 200 to 500 times in cellular DNA. Ad12 DNA and the recombinant genomes exhibit the same or similar lengths. This finding suggests that a constant amount of DNA is packaged into complete Ad12 particles. On cleavage of KB cellular DNA with EcoRI, BamHI, HinfI, Msp I, Mbo I Pst I, or Bgl II, the (32)P-labeled cellular DNA from Ad12-3a particles hybridizes on Southern blots to distinct bands of KB DNA. There is also less-specific background hybridization that is not observed in the control. The cellular DNA from Ad12-3a particles is not methylated, whereas the same cellular sequences in KB cell DNA appear to be extensively methylated. On denaturation and renaturation, the recombinant DNA molecules are converted to molecules half as long as Ad12 DNA, as determined by gel electrophoresis and electron microscopy. The recombinant DNA molecules were terminally labeled by exonuclease III treatment and subsequent refilling of the depleted segments with [(32)P]dNTPs by using DNA polymerase I (Klenow fragment). When these molecules were cleaved with EcoRI, BamHI, Msp I, or Pst I, only one terminal DNA fragment was found to be labeled. The results of partial digestion experiments using Msp I, HinfI, or Mbo I are consistent with a model in which 700-1150 base pairs from the left terminus of Ad12 DNA are linked to host cell DNA containing repetitious sequences, and this structure is symmetrically duplicated as a large inverted repeat of the type ABCDD'C'B'A'. The Ad12 DNA sequences are flanking the entire molecule, which consists mainly of human KB cell DNA. The recombinants appear to be stable on serial passage of the virus preparation for many years, although variations in the sequence of the recombinants occur. These symmetric recombinant (SYREC) molecules suggest a way to use adenovirus DNA as a eukaryotic vector. Their occurrence provides further evidence for the generation of virus-host DNA recombinants and may help elucidate the role this interaction may have in adenovirus replication and oncogenesis.

Cited by

References

  1. Biochemistry. 1980 Sep 30;19(20):4556-64 - PubMed
  2. J Mol Biol. 1977 Jun 15;113(1):237-51 - PubMed
  3. J Gen Virol. 1973 Sep;20(3):287-302 - PubMed
  4. Eur J Biochem. 1971 Oct 14;22(3):371-81 - PubMed
  5. Genetics. 1977 Feb;85(2):289-302 - PubMed
  6. Eur J Immunol. 1973 Dec;3(12):754-61 - PubMed
  7. Nucleic Acids Res. 1980 Jun 11;8(11):2461-73 - PubMed
  8. Nature. 1977 Feb 17;265(5595):643-5 - PubMed
  9. Science. 1974 Feb 22;183(4126):757-9 - PubMed
  10. Virology. 1972 Feb;47(2):507-12 - PubMed
  11. J Virol. 1980 Oct;36(1):22-40 - PubMed
  12. McGill Med J. 1950 Dec;19(4):245-9 - PubMed
  13. Teratology. 1977 Jun;15(3):249-51 - PubMed
  14. J Virol. 1978 Apr;26(1):61-70 - PubMed
  15. Cell. 1977 Sep;12(1):243-9 - PubMed
  16. J Virol. 1972 Feb;9(2):309-16 - PubMed
  17. Virology. 1972 Sep;49(3):745-57 - PubMed
  18. Virology. 1980 Feb;101(1):72-80 - PubMed
  19. Cell. 1980 Jul;20(3):787-95 - PubMed
  20. Virology. 1974 Aug;60(2):419-30 - PubMed
  21. J Virol. 1974 May;13(5):975-92 - PubMed
  22. Virology. 1973 Aug;54(2):384-97 - PubMed
  23. Virology. 1968 Mar;34(3):391-401 - PubMed
  24. J Virol. 1972 Feb;9(2):297-308 - PubMed
  25. Contemp Top Immunobiol. 1980;9:231-53 - PubMed
  26. Am J Med. 1972 Jan;52(1):1-8 - PubMed
  27. J Virol. 1981 Mar;37(3):887-92 - PubMed
  28. Transplantation. 1973 Jan;15(1):137-53 - PubMed
  29. Nature. 1978 Sep 14;275(5676):136-8 - PubMed
  30. J Exp Med. 1972 Nov 1;136(5):1231-40 - PubMed
  31. J Mol Biol. 1975 Nov 5;98(3):503-17 - PubMed
  32. Virology. 1969 Aug;38(4):587-606 - PubMed
  33. Nucleic Acids Res. 1980 Apr 11;8(7):1459-73 - PubMed
  34. Proc Natl Acad Sci U S A. 1976 Nov;73(11):3923-7 - PubMed
  35. Proc Soc Exp Biol Med. 1978 Sep;158(4):618-21 - PubMed
  36. Biochemistry. 1980 Jan 8;19(1):49-54 - PubMed
  37. Proc Natl Acad Sci U S A. 1980 Jan;77(1):253-6 - PubMed
  38. Cold Spring Harb Symp Quant Biol. 1980;44 Pt 1,:551-64 - PubMed
  39. J Virol. 1971 Apr;7(4):426-33 - PubMed
  40. Cell. 1979 Jul;17(3):705-13 - PubMed
  41. Nucleic Acids Res. 1976 Sep;3(9):2387-98 - PubMed
  42. J Biol Chem. 1964 Jan;239:251-8 - PubMed
  43. Teratology. 1975 Oct;12(2):165-71 - PubMed
  44. Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:77-90 - PubMed
  45. J Virol. 1973 Sep;12(3):492-500 - PubMed
  46. Proc R Soc Lond B Biol Sci. 1978 Jun 5;202(1146):117-58 - PubMed
  47. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 - PubMed
  48. Proc Natl Acad Sci U S A. 1975 Jul;72(7):2644-8 - PubMed
  49. Genetics. 1954 Mar;39(2):185-96 - PubMed
  50. Lancet. 1974 Jun 22;1(7869):1269-75 - PubMed
  51. Experientia. 1979 Aug 15;35(8):1103-4 - PubMed
  52. Pediatrics. 1957 Apr;19(4 Part 2):782-7 - PubMed
  53. Adv Immunol. 1974;18:1-66 - PubMed
  54. J Immunol. 1969 Jul;103(1):66-70 - PubMed
  55. J Mol Biol. 1969 Sep 14;44(2):333-45 - PubMed
  56. Cell. 1978 Jul;14(3):569-85 - PubMed
  57. Pediatrics. 1951 Oct;8(4):527-33 - PubMed
  58. J Virol. 1979 Oct;32(1):240-50 - PubMed
  59. Virology. 1978 May 1;86(1):66-77 - PubMed
  60. Science. 1972 Jan 21;175(4019):273-9 - PubMed
  61. Differentiation. 1979;13(3):141-54 - PubMed

Substances

MeSH terms

Publication Types

LinkOut - more resources