Display options
Cite
Hølund B, Clausen PP, Clemmensen I. The influence of fixation and tissue preparation on the immunohistochemical demonstration of fibronectin in human tissue. Histochemistry. 1981;72(2):291-9doi: 10.1007/BF00517142.
Hølund, B., Clausen, P. P., & Clemmensen, I. (1981). The influence of fixation and tissue preparation on the immunohistochemical demonstration of fibronectin in human tissue. Histochemistry, 72(2), 291-9. https://doi.org/10.1007/BF00517142
Hølund, B, et al. "The influence of fixation and tissue preparation on the immunohistochemical demonstration of fibronectin in human tissue." Histochemistry vol. 72,2 (1981): 291-9. doi: https://doi.org/10.1007/BF00517142
Hølund B, Clausen PP, Clemmensen I. The influence of fixation and tissue preparation on the immunohistochemical demonstration of fibronectin in human tissue. Histochemistry. 1981;72(2):291-9. doi: 10.1007/BF00517142. PMID: 6168612.
Copy Download .nbib Format: NLM
Share it on

Histochemistry. 1981;72(2):291-9. doi: 10.1007/BF00517142.

The influence of fixation and tissue preparation on the immunohistochemical demonstration of fibronectin in human tissue.

Histochemistry

B Hølund, P P Clausen, I Clemmensen

PMID: 6168612 DOI: 10.1007/BF00517142

Abstract

The influence of fixation and tissue preparation on the immunohistochemical localization of human fibronectin in gastrointestinal tract tissue has been examined using indirect immunoperoxidase technique. The most optimal staining result with strong intensity and well defined localization was obtained on frozen sections of unfixed material. Nearly identical results with improved morphology were obtained when staining paraffin sections of tissue fixed in 96% ethanol, 96% + 1% acetic acid and absolute acetone. All other fixatives tested, 10% neutral buffered formalin. Lillie's AAF, Bouin's fixative, Clarke's fixative, 4% formaldehyde, 4% formaldehyde + 0.5% cetylpyridiniumchloride (F-CPC), 4% formaldehyde +0.1% glutaraldehyde gave unsatisfactory results. However, proteolytic digestion with pepsin of paraffin sections prior to staining of buffered formalin and F-CPCfixed material gave results comparable with those obtained on unfixed frozen sections are regards definition of the staining whereas staining intensity was decreased in some degree. No improvement was observed when using proteolytic digestion of tissue fixed in other fixatives.

References

  1. J Biol Chem. 1970 Nov 10;245(21):5728-36 - PubMed
  2. Arch Dermatol Res. 1979 Aug;266(1):33-41 - PubMed
  3. Gut. 1980 Jan;21(1):63-71 - PubMed
  4. Scand J Immunol Suppl. 1973;1:161-4 - PubMed
  5. Br J Dermatol. 1979 Sep;101(3):263-70 - PubMed
  6. J Exp Med. 1978 Apr 1;147(4):1054-64 - PubMed
  7. Lab Invest. 1980 Jul;43(1):47-51 - PubMed
  8. Acta Derm Venereol. 1979;59(6):487-92 - PubMed
  9. Arthritis Rheum. 1982 Jan;25(1):25-31 - PubMed
  10. Acta Pathol Microbiol Scand A. 1978 Sep;86A(5):383-8 - PubMed
  11. J Clin Pathol. 1980 Nov;33(11):1021-8 - PubMed
  12. Clin Immunol Immunopathol. 1978 Dec;11(4):425-36 - PubMed
  13. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1217-21 - PubMed
  14. Proc Soc Exp Biol Med. 1972 May;140(1):199-204 - PubMed
  15. Lab Invest. 1979 Oct;41(4):340-7 - PubMed
  16. Arch Dermatol Res. 1979 Nov;266(3):295-310 - PubMed
  17. J Exp Med. 1978 Jun 1;147(6):1584-95 - PubMed
  18. Blood. 1980 Aug;56(2):145-58 - PubMed
  19. Arthritis Rheum. 1979 Sep;22(9):983-7 - PubMed
  20. Ann N Y Acad Sci. 1978 Jun 20;312:151-9 - PubMed
  21. J Cell Biol. 1978 Oct;79(1):263-7 - PubMed
  22. Histochemistry. 1980;67(1):73-8 - PubMed

Substances

MeSH terms

Publication Types