Display options
Cite
Chikhaoui Y, Boussioux AM, Nicolas JC, et al. Enzymatic determination of dehydroepiandrosterone sulfate in biological fluids. J Steroid Biochem. 1983;19(3):1319-23doi: 10.1016/0022-4731(83)90157-7.
Chikhaoui, Y., Boussioux, A. M., Nicolas, J. C., Descomps, B., & de Paulet, A. C. (1983). Enzymatic determination of dehydroepiandrosterone sulfate in biological fluids. Journal of steroid biochemistry, 19(3), 1319-23. https://doi.org/10.1016/0022-4731(83)90157-7
Chikhaoui, Y, et al. "Enzymatic determination of dehydroepiandrosterone sulfate in biological fluids." Journal of steroid biochemistry vol. 19,3 (1983): 1319-23. doi: https://doi.org/10.1016/0022-4731(83)90157-7
Chikhaoui Y, Boussioux AM, Nicolas JC, Descomps B, de Paulet AC. Enzymatic determination of dehydroepiandrosterone sulfate in biological fluids. J Steroid Biochem. 1983 Sep;19(3):1319-23. doi: 10.1016/0022-4731(83)90157-7. PMID: 6225910.
Copy Download .nbib Format: NLM
Share it on

J Steroid Biochem. 1983 Sep;19(3):1319-23. doi: 10.1016/0022-4731(83)90157-7.

Enzymatic determination of dehydroepiandrosterone sulfate in biological fluids.

Journal of steroid biochemistry

Y Chikhaoui, A M Boussioux, J C Nicolas, B Descomps, A C de Paulet

PMID: 6225910 DOI: 10.1016/0022-4731(83)90157-7

Abstract

A simple method is described for the determination of androgens, particularly dehydroepiandrosterone sulfate (DHEAS), in human plasma and urine. The method does not involve extraction or chromatography. An internal standard is used as reference. The androgens are enzymatically converted to estrogens and these latter compounds can be measured by the enzymatic method previously described [1]. The range of accuracy was between 99 and 103%. The precision of the method was 2.5%. The sensitivity was 0.1 pM per sample. The method is suitable for routine use, and one worker can easily perform twenty assays in one day.

Substances

MeSH terms

Publication Types