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De Villiers S, Van Der Walt JG, Procos J. An accurate, sensitive and reproducible method for the colorimetric estimation of free fatty acids in plasma. Onderstepoort J Vet Res. 1977;44(3):169-72
De Villiers, S., Van Der Walt, J. G., & Procos, J. (1977). An accurate, sensitive and reproducible method for the colorimetric estimation of free fatty acids in plasma. The Onderstepoort journal of veterinary research, 44(3), 169-72.
De Villiers, S, et al. "An accurate, sensitive and reproducible method for the colorimetric estimation of free fatty acids in plasma." The Onderstepoort journal of veterinary research vol. 44,3 (1977): 169-72.
De Villiers S, Van Der Walt JG, Procos J. An accurate, sensitive and reproducible method for the colorimetric estimation of free fatty acids in plasma. Onderstepoort J Vet Res. 1977 Sep;44(3):169-72. PMID: 614534.
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Onderstepoort J Vet Res. 1977 Sep;44(3):169-72.

An accurate, sensitive and reproducible method for the colorimetric estimation of free fatty acids in plasma.

The Onderstepoort journal of veterinary research

S De Villiers, J G Van Der Walt, J Procos

PMID: 614534

Abstract

A sensitive and reproducible method permitting the estimation of 0,030-3,000 mM free fatty acids (FFA) in plasma with a standard error of 0,006 5 was developed. The method is based on the extraction of phosphate-buffered plasma with a chloroform-heptane-methanol mixture, formation of the cobalt complex and on the subsequent determination of the metal with 1-nitroso-2-naphthol. The method was used to obtain normal values (mean 0,547 mM range 0,147-1,197 mM) for Merino wethers fed lucerne hay.

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