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Kühner U, Barandun S, Morell A, et al. Differentiation of plasma and myeloma cells of man. Combined planimetric and cytophotometric studies. Klin Wochenschr. 1977;55(12):573-6doi: 10.1007/BF01490510.
Kühner, U., Barandun, S., Morell, A., Skvaril, F., & Wagner, H. P. (1977). Differentiation of plasma and myeloma cells of man. Combined planimetric and cytophotometric studies. Klinische Wochenschrift, 55(12), 573-6. https://doi.org/10.1007/BF01490510
Kühner, U, et al. "Differentiation of plasma and myeloma cells of man. Combined planimetric and cytophotometric studies." Klinische Wochenschrift vol. 55,12 (1977): 573-6. doi: https://doi.org/10.1007/BF01490510
Kühner U, Barandun S, Morell A, Skvaril F, Wagner HP. Differentiation of plasma and myeloma cells of man. Combined planimetric and cytophotometric studies. Klin Wochenschr. 1977 Jun 15;55(12):573-6. doi: 10.1007/BF01490510. PMID: 69746.
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Klin Wochenschr. 1977 Jun 15;55(12):573-6. doi: 10.1007/BF01490510.

Differentiation of plasma and myeloma cells of man. Combined planimetric and cytophotometric studies.

Klinische Wochenschrift

U Kühner, S Barandun, A Morell, F Skvaril, H P Wagner

PMID: 69746 DOI: 10.1007/BF01490510

Abstract

Plasmacytoid cells in the bone marrow of 3 patients with myeloma and plasma cells in the bone marrow of a 6-year-old boy with an infectious disease were assessed cytophotometrically, first after Giemsaand second after Feulgen staining. The cell and nuclear surface and the nuclear/cytoplasmic ratio were determined from the number of measuring points. The nuclear DNA content of individual cells was registered and the distribution of DNA within the nucleus was assessed by the distributional error. Both the mean nuclear/cytoplasmic ratio and the distributional error of myeloma cells varied from patient to patient but could not be used to differentiate between normal plasma cells and myeloma cells. It was not possible either to differentiate these cell types by multiplying the mean nuclear/cytoplasmic ratio with the mean distributional error of the nuclear DNA. A strong correlation between cell and cytoplasmic surface area was observed both in normal plasma cells and in myeloma cells.

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