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Am J Vet Res. 1985 Sep;46(9):1971-4.

Bovine neutrophils treated with chemotactic agents: morphologic changes.

American journal of veterinary research

J H Forsell, J R Kateley, C W Smith

PMID: 3901840

Abstract

Neutrophils were isolated from the peripheral blood of cattle. After the neutrophils were incubated with zymosan-activated serum, the neutrophils changed from spherical to a bipolar shape. Ninety percent of the neutrophils became bipolar in 5 to 10 minutes. Bacterial cell filtrate and casein also induced bipolar shape changes in neutrophils, but N-formyl-L-methionyl-L-leucinyl-L-phenylalanine did not. The neutrophil-shape-change response was a rapid in vitro assay to evaluate early chemotactic events.

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