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Erlichman C, Tannock IF. Growth and characterization of multicellular tumor spheroids of human bladder carcinoma origin. In Vitro Cell Dev Biol. 1986;22(8):449-56doi: 10.1007/BF02623445.
Erlichman, C., & Tannock, I. F. (1986). Growth and characterization of multicellular tumor spheroids of human bladder carcinoma origin. In vitro cellular & developmental biology : journal of the Tissue Culture Association, 22(8), 449-56. https://doi.org/10.1007/BF02623445
Erlichman, C, and Tannock, I F. "Growth and characterization of multicellular tumor spheroids of human bladder carcinoma origin." In vitro cellular & developmental biology : journal of the Tissue Culture Association vol. 22,8 (1986): 449-56. doi: https://doi.org/10.1007/BF02623445
Erlichman C, Tannock IF. Growth and characterization of multicellular tumor spheroids of human bladder carcinoma origin. In Vitro Cell Dev Biol. 1986 Aug;22(8):449-56. doi: 10.1007/BF02623445. PMID: 3733640.
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In Vitro Cell Dev Biol. 1986 Aug;22(8):449-56. doi: 10.1007/BF02623445.

Growth and characterization of multicellular tumor spheroids of human bladder carcinoma origin.

In vitro cellular & developmental biology : journal of the Tissue Culture Association

C Erlichman, I F Tannock

PMID: 3733640 DOI: 10.1007/BF02623445

Abstract

We have examined the MGH-U1 human bladder carcinoma cell line and 12 primary bladder carcinoma biopsies for their ability to form spheroids in suspension culture and in multiwell dishes. MGH-U1 cells formed tightly packed spheroids with a necrotic center and viable rim whereas three sublines formed loose aggregates only. Spheroids formed from as few as 100 MGH-U1 cells placed into multiwells. MGH-U1 cells derived from spheroids formed new spheroids more rapidly and and consistently than cells derived from monolayer culture. Spheroid diameter increased at a rapid rate of approximately 100 microns/d in multiwell dishes, and necrosis occurred only in spheroids of diameter greater than 1 mm. Spheroids placed in spinner culture at a higher concentration (approximately 1.5 spheroids/ml) grew more slowly and developed necrosis at smaller diameters. The width of the viable rim of spheroids grown in spinner culture was maintained at approximately 190 microns over a wide range of spheroid diameters (400 to 1000 microns). Sequential trypsinization of spheroids, which stripped layers of cells from the spheroids, demonstrated no difference in the plating efficiency of cells derived from varying depths into the spheroid. Only one of the 12 primary bladder biopsy specimens demonstrated an ability to form spheroids. This biopsy, designated HB-10, formed spheroids that grew linearly over 40 d, formed colonies in methylcellulose culture and grew as xenografts in immune-deprived mice. These studies characterize the MGH-U1 spheroids that are useful in vitro models to study the effects of various treatments for solid tumors and demonstrate the limited capacity of cells from primary human bladder biopsies to form spheroids.

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References

  1. Nat New Biol. 1971 Mar 10;230(10):52-4 - PubMed
  2. Tohoku J Exp Med. 1977 Feb;121(2):157-64 - PubMed
  3. Br J Cancer. 1978 Feb;37(2):224-30 - PubMed
  4. Cancer Res. 1980 Nov;40(11):3956-65 - PubMed
  5. J Cell Physiol. 1981 Jan;106(1):23-32 - PubMed
  6. Cancer Res. 1984 Nov;44(11):5369-75 - PubMed
  7. Int J Radiat Biol Relat Stud Phys Chem Med. 1973 Mar;23(3):235-46 - PubMed
  8. J Histochem Cytochem. 1982 Feb;30(2):117-22 - PubMed
  9. Tohoku J Exp Med. 1978 Apr;124(4):339-49 - PubMed
  10. Exp Cell Res. 1972 Mar;71(1):75-80 - PubMed
  11. Cancer Res. 1984 Jun;44(6):2530-3 - PubMed
  12. Int J Cancer. 1981 Jan 15;27(1):15-21 - PubMed
  13. Cancer Res. 1982 Sep;42(9):3696-703 - PubMed
  14. Cancer. 1982 Nov 15;50(10):2066-73 - PubMed
  15. Cancer. 1981 May 15;47(10):2349-57 - PubMed
  16. Growth. 1966 Jun;30(2):157-76 - PubMed
  17. J Natl Cancer Inst. 1971 Jan;46(1):113-20 - PubMed
  18. Radiat Res. 1981 May;86(2):368-86 - PubMed
  19. Cell Tissue Kinet. 1976 Sep;9(5):403-12 - PubMed
  20. Nature. 1983 Feb 3;301(5899):429-30 - PubMed
  21. Cancer Res. 1977 Oct;37(10):3639-43 - PubMed
  22. Cancer Res. 1978 May;38(5):1457-64 - PubMed
  23. Radiat Res. 1979 Sep;79(3):439-53 - PubMed

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