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J Appl Bacteriol. 1986 Jun;60(6):491-500. doi: 10.1111/j.1365-2672.1986.tb01088.x.

Estimation of gram-negative bacteria in milk: a comparison of inhibitor systems for preventing gram-positive bacterial growth.

The Journal of applied bacteriology

J D Phillips, M W Griffiths

PMID: 3745029 DOI: 10.1111/j.1365-2672.1986.tb01088.x

Abstract

A number of inhibitor systems which have been reported to allow selection of Gram-negative bacteria were tested against Gram-positive and Gram-negative isolates of dairy origin. No one system worked perfectly. A mixture of crystal violet-penicillin-nisin or monensin had least inhibitory effect on Gram-negative isolates whereas Selectocult (a commercially available mixture of Benzalkon A 50% and crystal violet) and sodium deoxycholate were the most effective inhibitors of Gram-positive bacteria. Cetrimide-fucidin-cephaloridine solutions, which have been reported as allowing selective growth of pseudomonads, were not so specific when applied to milk systems.

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