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James J, Bosch KS, Zuyderhoudt FM, et al. Histophotometric estimation of volume density of collagen as an indication of fibrosis in rat liver. Histochemistry. 1986;85(2):129-33doi: 10.1007/BF00491759.
James, J., Bosch, K. S., Zuyderhoudt, F. M., Houtkooper, J. M., & van Gool, J. (1986). Histophotometric estimation of volume density of collagen as an indication of fibrosis in rat liver. Histochemistry, 85(2), 129-33. https://doi.org/10.1007/BF00491759
James, J, et al. "Histophotometric estimation of volume density of collagen as an indication of fibrosis in rat liver." Histochemistry vol. 85,2 (1986): 129-33. doi: https://doi.org/10.1007/BF00491759
James J, Bosch KS, Zuyderhoudt FM, Houtkooper JM, van Gool J. Histophotometric estimation of volume density of collagen as an indication of fibrosis in rat liver. Histochemistry. 1986;85(2):129-33. doi: 10.1007/BF00491759. PMID: 3744896.
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Histochemistry. 1986;85(2):129-33. doi: 10.1007/BF00491759.

Histophotometric estimation of volume density of collagen as an indication of fibrosis in rat liver.

Histochemistry

J James, K S Bosch, F M Zuyderhoudt, J M Houtkooper, J van Gool

PMID: 3744896 DOI: 10.1007/BF00491759

Abstract

The development of fibrosis in the liver of 16 rats treated for 1, 2, 3 or 4 weeks with CCl4, has been followed with chemical hydroxyproline determination and histophotometric analysis of histological sections stained with Sirius Red F3BA in saturated aqueous picric acid. The readings were taken with a scanning and integrating microphotometer and corrected for picric acid absorbance as a measure for mean protein mass per unit area of the section. It appears that the integrated absorbance readings of Sirius Red absorbing material in the section show a highly significant correlation with the hydroxyproline determinations. It is concluded that picrosirius photometry can be used to give a measure of the volume density of collagen in sections. An advantage of the photometric assay is that measurements are taken on the basis of the microscopic image, so that it is also possible to estimate collagen density in a selected area, e.g. a tumour formation amidst normal tissue, or to exclude necrotic areas.

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