Display options
Cite
Schindler D, Kubbies M, Hoehn H, et al. Confirmation of Fanconi's anemia and detection of a chromosomal aberration (1Q12-32 triplication) via BrdU/Hoechst flow cytometry. Am J Pediatr Hematol Oncol. 1987;9(2):172-7doi: 10.1097/00043426-198722000-00014.
Schindler, D., Kubbies, M., Hoehn, H., Schinzel, A., & Rabinovitch, P. S. (1987). Confirmation of Fanconi's anemia and detection of a chromosomal aberration (1Q12-32 triplication) via BrdU/Hoechst flow cytometry. The American journal of pediatric hematology/oncology, 9(2), 172-7. https://doi.org/10.1097/00043426-198722000-00014
Schindler, D, et al. "Confirmation of Fanconi's anemia and detection of a chromosomal aberration (1Q12-32 triplication) via BrdU/Hoechst flow cytometry." The American journal of pediatric hematology/oncology vol. 9,2 (1987): 172-7. doi: https://doi.org/10.1097/00043426-198722000-00014
Schindler D, Kubbies M, Hoehn H, Schinzel A, Rabinovitch PS. Confirmation of Fanconi's anemia and detection of a chromosomal aberration (1Q12-32 triplication) via BrdU/Hoechst flow cytometry. Am J Pediatr Hematol Oncol. 1987;9(2):172-7. doi: 10.1097/00043426-198722000-00014. PMID: 3592130.
Copy Download .nbib Format: NLM
Share it on

Am J Pediatr Hematol Oncol. 1987;9(2):172-7. doi: 10.1097/00043426-198722000-00014.

Confirmation of Fanconi's anemia and detection of a chromosomal aberration (1Q12-32 triplication) via BrdU/Hoechst flow cytometry.

The American journal of pediatric hematology/oncology

D Schindler, M Kubbies, H Hoehn, A Schinzel, P S Rabinovitch

PMID: 3592130 DOI: 10.1097/00043426-198722000-00014

Abstract

Peripheral blood lymphocyte cultures of a patient with clinical evidence of Fanconi's anemia (FA) were investigated by means of BrdU/Hoechst flow cytometry. This technique can be used to differentiate cycling from noncycling cells after PHA stimulation; it also permits the quantitative assessment of cell cycle progression and cell-cycle arrest. A characteristic cell kinetic pattern of combined G2 phase prolongation and G2 phase arrest was observed in the present patient, confirming the clinical diagnosis of FA. An additional finding was the presence of a small subpopulation of peripheral blood cells, refractory to PHA stimulation, and displaying a DNA content 5% greater than that of the diploid cells. BrdU/Hoechst flow cytometry was crucial to the unequivocal demonstration of this subpopulation, which would not have been detected without enrichment by sequential depletion of PHA-responsive cells from the G0/G1 compartment during a 96-h stimulation experiment. The parallel finding of a presumptive triplication affecting the q12-32 segment of chromosome 1 in rare spontaneous divisions of a 40-h peripheral blood culture is consistent with the flow-cytometric detection of elevated DNA content if, as is common in FA, the abnormal cell lineage represents a preleukemic clone of monocytic origin.

Cited by

MeSH terms

Publication Types

Grant support

LinkOut - more resources