J Laryngol Otol. 1979 Jan;93(1):7-23. doi: 10.1017/s0022215100086679.

An evaluation of the use of scanning and transmission electronmicroscopy in a study of the gentamicin-damaged guinea-pig organ of Corti.

The Journal of laryngology and otology

E S Harpur, J B Bridges

PMID: 429887 DOI: 10.1017/s0022215100086679

Abstract

Scanning electronmicroscopy and transmission electronmicroscopy were used to study the normal and gentamicin-damaged guinea-pig organ of Corti. Scanning electronmicroscopy was principally used to locate the lesion and quantify the cell loss but also permitted and detailed study to be made of the degenerative surface changes. However, since consistent surface changes are not seen until intracellular degeneration has progressed to a great extent, scanning electronmicroscopy is not a suitable technique for identifying early degenerative changes in the sensory cells of the organ of Corti. Consequently, before conclusions may be drawn about the absence of pathological changes in the neuro-epithelium it also should be examined using transmission electron-miscroscopy.

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Substances

• Gentamicins

MeSH terms

• Animals
• Dose-Response Relationship, Drug
• Gentamicins / toxicity
• Guinea Pigs
• Male
• Microscopy, Electron*
• Microscopy, Electron, Scanning
• Mitochondria / ultrastructure
• Organ of Corti / drug effects
• Organ of Corti / ultrastructure

Publication Types

• Journal Article