Display options
Cite
Silva SS, Ribeiro MO, Gomes FI, et al. Occurrence and antimicrobial susceptibility of enteric rods and pseudomonads isolated from the dental prostheses biofilm. J Appl Oral Sci. 2016;24(5):462-471doi: 10.1590/1678-775720160032.
Silva, S. S., Ribeiro, M. O., Gomes, F. I., Chaves, H. V., Silva, A. A., Zanin, I. C., & Barbosa, F. C. (2016). Occurrence and antimicrobial susceptibility of enteric rods and pseudomonads isolated from the dental prostheses biofilm. Journal of applied oral science : revista FOB, 24(5), 462-471. https://doi.org/10.1590/1678-775720160032
Silva, Sanrrangers Sales, et al. "Occurrence and antimicrobial susceptibility of enteric rods and pseudomonads isolated from the dental prostheses biofilm." Journal of applied oral science : revista FOB vol. 24,5 (2016): 462-471. doi: https://doi.org/10.1590/1678-775720160032
Silva SS, Ribeiro MO, Gomes FI, Chaves HV, Silva AA, Zanin IC, Barbosa FC. Occurrence and antimicrobial susceptibility of enteric rods and pseudomonads isolated from the dental prostheses biofilm. J Appl Oral Sci. 2016 Sep-Oct;24(5):462-471. doi: 10.1590/1678-775720160032. PMID: 27812616; PMCID: PMC5083023.
Copy Download .nbib Format: NLM
Share it on

J Appl Oral Sci. 2016 Sep-Oct;24(5):462-471. doi: 10.1590/1678-775720160032.

Occurrence and antimicrobial susceptibility of enteric rods and pseudomonads isolated from the dental prostheses biofilm.

Journal of applied oral science : revista FOB

Sanrrangers Sales Silva, Maximilo de Oliveira Ribeiro, Francisco Isaac Fernandes Gomes, Hellíada Vasconcelos Chaves, Antonio Alfredo Rodrigues E Silva, Iriana Carla Junqueira Zanin, Francisco Cesar Barroso Barbosa

Affiliations

  1. Universidade Federal do Ceará, Faculdade de Odontologia, Campus Sobral, Sobral, CE, Brasil.
  2. Universidade Federal do Ceará, Faculdade de Medicina, Campus Sobral,Sobral, CE, Brasil.
  3. Agência Nacional de Vigilância Sanitária, Brasília, DF, Brasil.

PMID: 27812616 PMCID: PMC5083023 DOI: 10.1590/1678-775720160032

Abstract

OBJECTIVE:: To determine the occurrence and the in vitro antimicrobial susceptibility of enteric rods and pseudomonads from the denture biofilm of 52 subjects at the Center for Dental Specialties of Sobral/ Ceara, Brazil.

MATERIAL AND METHODS:: Denture biofilm was collected and samples plated on MacConkey agar. The isolated bacterial colonies were identified using the BBL Crystal enteric/non-fermenter system. Antibiotic bacterial susceptibility was assessed by the disc diffusion method of amoxicillin, amoxicillin/clavulanic acid, doxycycline, tetracycline, tobramycin, imipenem, cefotaxime, and ciprofloxacin. The Minimum Inhibitory Concentration (MIC) of cefotaxime, tobramycin, doxycycline, imipenem, and ciprofloxacin was determined for 40 species by E-test.

RESULTS:: 34 subjects (65.4%) harbored enteric rods in their prostheses. Klebsiella pneumoniae (26.5%), Escherichia coli (23.5%), and Enterobacter aerogenes (23.5%) were the most prevalent species. All organisms were susceptible to ciprofloxacin and most species were resistant to amoxicillin or amoxicillin/clavulanic acid, demonstrating variable sensitivity patterns to other antimicrobials. However, the MIC showed the emergence of strains with reduced sensitivity to ciprofloxacin (MIC90≥3 μg/ mL) and cefotaxime (MIC90≥2 μg/mL).

CONCLUSION:: The findings show high prevalence of nosocomial diseases-related bacterial species and low susceptibility to antimicrobial drugs. Therefore, these results imply caution against the indiscriminate use of broad spectrum antibiotics in dental practice.

References

  1. Braz J Infect Dis. 2005 Feb;9(1):44-51 - PubMed
  2. J Appl Oral Sci. 2009;17 Suppl:13-8 - PubMed
  3. J Clin Periodontol. 1996 Feb;23(2):133-9 - PubMed
  4. Am J Public Health. 2012 Mar;102(3):411-8 - PubMed
  5. Oral Microbiol Immunol. 2006 Feb;21(1):53-60 - PubMed
  6. Proc Natl Acad Sci U S A. 2006 Mar 7;103(10):3828-33 - PubMed
  7. Clin Microbiol Rev. 2012 Oct;25(4):682-707 - PubMed
  8. Br J Biomed Sci. 2007;64(4):180-9 - PubMed
  9. Ann Clin Microbiol Antimicrob. 2016 May 04;15:29 - PubMed
  10. J Prosthodont. 2011 Feb;20 Suppl 1:S1-S12 - PubMed
  11. J Periodontol. 1991 Sep;62(9):543-7 - PubMed
  12. Proc Natl Acad Sci U S A. 2008 Feb 26;105(8):3100-5 - PubMed
  13. Gerodontology. 2003 Dec;20(2):84-7 - PubMed
  14. Gerodontology. 2005 Sep;22(3):158-67 - PubMed
  15. Gerodontology. 2006 Mar;23(1):3-16 - PubMed
  16. Gerodontology. 2012 Jun;29(2):e768-71 - PubMed
  17. Oral Microbiol Immunol. 1990 Jun;5(3):149-54 - PubMed
  18. J Dent. 2013 May;41(5):420-7 - PubMed
  19. J Clin Diagn Res. 2016 Mar;10(3):DC05-9 - PubMed
  20. J Dent Res. 1992 Jul;71(7):1374-81 - PubMed
  21. J Antimicrob Chemother. 2008 Aug;62(2):369-75 - PubMed
  22. Gerodontology. 2002 Jul;19(1):25-9 - PubMed
  23. Braz J Microbiol. 2012 Oct;43(4):1309-14 - PubMed
  24. Gerodontology. 2016 Sep;33(3):322-7 - PubMed
  25. J Dent Res. 1997 Nov;76(11):1770-5 - PubMed
  26. J Microbiol Immunol Infect. 2009 Aug;42(4):317-23 - PubMed
  27. J Prosthodont. 2016 Feb;25(2):99-104 - PubMed
  28. J Infect Chemother. 2015 Feb;21(2):105-9 - PubMed
  29. Adv Med Sci. 2006;51 Suppl 1:86-90 - PubMed
  30. J Appl Oral Sci. 2010 Dec;18(6):551-9 - PubMed

Substances

MeSH terms

Publication Types