Display options
Cite
Pantaleon M, Harvey MB, Pascoe WS, et al. Glucose transporter GLUT3: ontogeny, targeting, and role in the mouse blastocyst. Proc Natl Acad Sci U S A. 1997;94(8):3795-800doi: 10.1073/pnas.94.8.3795.
Pantaleon, M., Harvey, M. B., Pascoe, W. S., James, D. E., & Kaye, P. L. (1997). Glucose transporter GLUT3: ontogeny, targeting, and role in the mouse blastocyst. Proceedings of the National Academy of Sciences of the United States of America, 94(8), 3795-800. https://doi.org/10.1073/pnas.94.8.3795
Pantaleon, M, et al. "Glucose transporter GLUT3: ontogeny, targeting, and role in the mouse blastocyst." Proceedings of the National Academy of Sciences of the United States of America vol. 94,8 (1997): 3795-800. doi: https://doi.org/10.1073/pnas.94.8.3795
Pantaleon M, Harvey MB, Pascoe WS, James DE, Kaye PL. Glucose transporter GLUT3: ontogeny, targeting, and role in the mouse blastocyst. Proc Natl Acad Sci U S A. 1997 Apr 15;94(8):3795-800. doi: 10.1073/pnas.94.8.3795. PMID: 9108057; PMCID: PMC20520.
Copy Download .nbib Format: NLM
Share it on

Proc Natl Acad Sci U S A. 1997 Apr 15;94(8):3795-800. doi: 10.1073/pnas.94.8.3795.

Glucose transporter GLUT3: ontogeny, targeting, and role in the mouse blastocyst.

Proceedings of the National Academy of Sciences of the United States of America

M Pantaleon, M B Harvey, W S Pascoe, D E James, P L Kaye

Affiliations

  1. Department of Physiology and Pharmacology, The University of Queensland, Brisbane, Australia.

PMID: 9108057 PMCID: PMC20520 DOI: 10.1073/pnas.94.8.3795

Abstract

The first differentiative event in mammalian development is segregation of the inner cell mass and trophectoderm (TE) lineages. The epithelial TE cells pump fluid into the spherical blastocyst to form the blastocyst cavity. This activity is fuelled by glucose supplied through facilitative glucose transporters. However, the reported kinetic characteristics of blastocyst glucose transport are inconsistent with those of the previously identified transporters and suggested the presence of a high-affinity glucose carrier. We identified and localized the primary transporter in TE cells. It is glucose transporter GLUT3, previously described in the mouse as neuron-specific. In the differentiating embryo, GLUT3 is targeted to the apical membranes of the polarized cells of the compacted morula and then to the apical membranes of TE cells where it has access to maternal glucose. In contrast, GLUT1 was restricted to basolateral membranes of the outer TE cells in both compacted morulae and blastocysts. Using antisense oligodeoxynucleotides to specifically block protein expression, we confirmed that GLUT3 and not GLUT1 is the functional transporter for maternal glucose on the apical TE. More importantly, however, GLUT3 expression is required for blastocyst formation and hence this primary differentiation in mammalian development. This requirement is independent of its function as a glucose transporter because blastocysts will form in the absence of glucose. Thus the vectorial expression of GLUT3 into the apical membrane domains of the outer cells of the morula, which in turn form the TE cells of the blastocyst, is required for blastocyst formation.

References

  1. Proc Natl Acad Sci U S A. 1993 Nov 1;90(21):10023-5 - PubMed
  2. FEBS Lett. 1993 Nov 22;334(3):289-95 - PubMed
  3. FASEB J. 1994 Oct;8(13):1003-11 - PubMed
  4. Am J Physiol. 1995 May;268(5 Pt 1):C1077-89 - PubMed
  5. Mol Reprod Dev. 1995 Apr;40(4):436-43 - PubMed
  6. Reprod Fertil Dev. 1995;7(1):41-50 - PubMed
  7. Am J Physiol. 1996 Aug;271(2 Pt 1):C547-54 - PubMed
  8. J Exp Zool. 1965 Feb;158:59-68 - PubMed
  9. Development. 1991 Sep;113(1):363-72 - PubMed
  10. J Biol Chem. 1992 Jan 5;267(1):467-72 - PubMed
  11. Development. 1992 May;115(1):305-12 - PubMed
  12. Proc Natl Acad Sci U S A. 1992 Aug 15;89(16):7556-60 - PubMed
  13. J Reprod Fertil. 1965 Oct;10(2):227-40 - PubMed
  14. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
  15. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1056-60 - PubMed
  16. Nature. 1978 May 11;273(5658):149-51 - PubMed
  17. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 - PubMed
  18. J Reprod Fertil. 1984 Sep;72(1):9-13 - PubMed
  19. J Reprod Fertil. 1985 May;74(1):77-86 - PubMed
  20. Aust J Biol Sci. 1985;38(4):411-20 - PubMed
  21. J Reprod Fertil. 1986 Sep;78(1):149-57 - PubMed
  22. Scanning Microsc. 1988 Mar;2(1):417-26 - PubMed
  23. Nucleic Acids Res. 1989 Mar 25;17(6):2223-31 - PubMed
  24. Dev Biol. 1989 May;133(1):210-20 - PubMed
  25. Development. 1988 Nov;104(3):423-9 - PubMed
  26. J Reprod Fertil. 1990 May;89(1):1-11 - PubMed
  27. Mol Reprod Dev. 1990 Oct;27(2):81-92 - PubMed
  28. Am J Physiol. 1990 Dec;259(6 Pt 1):C279-85 - PubMed
  29. Am J Physiol. 1991 Mar;260(3 Pt 1):C570-80 - PubMed
  30. Biochemistry. 1991 May 28;30(21):5139-45 - PubMed
  31. Science. 1991 Nov 8;254(5033):847-50 - PubMed
  32. Diabetologia. 1992 Apr;35(4):304-9 - PubMed
  33. Biochim Biophys Acta. 1993 Jun 8;1154(1):17-49 - PubMed
  34. Nucleic Acids Res. 1993 Jun 25;21(12):2957-8 - PubMed
  35. Methods Enzymol. 1993;225:303-28 - PubMed

Substances

MeSH terms

Publication Types