J Chromatogr. 1987 Jun 26;397:355-64. doi: 10.1016/s0021-9673(01)85019-6.

Rapid dual-column chromatographic assay for recombinant leukocyte interferon alpha-2.

Journal of chromatography

L Rybacek, M D'Andrea, S J Tarnowski

PMID: 3308936 DOI: 10.1016/s0021-9673(01)85019-6

Abstract

A rapid dual-column chromatographic assay for determining recombinant leukocyte interferon alpha-2 in complex mixtures is described. The assay relies on the use of a high-performance monoclonal antibody affinity column connected in tandem with a reversed-phase column. The high specificity and selectivity of these columns permits the quantitation of subcomponent species, such as interferon oligomers that may be present in assay samples. The assay has a limit of sensitivity equal to 1 microgram/g over a range of 1-20 micrograms/g. The precision of the assay was estimated to be about 5%.

Substances

• Interferon Type I
• Recombinant Proteins
• Solutions
• Sodium Dodecyl Sulfate

MeSH terms

• Chromatography, Affinity
• Electrophoresis, Polyacrylamide Gel
• Escherichia coli / genetics
• Humans
• Immunosorbent Techniques
• Interferon Type I / analysis
• Interferon Type I / pharmacology
• Leukocytes / analysis
• Recombinant Proteins / analysis
• Saccharomyces cerevisiae / genetics
• Sodium Dodecyl Sulfate
• Solutions

Publication Types

• Journal Article

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