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Deeks S, Richards J, Nandi S. Maintenance of normal rat mammary epithelial cells by insulin and insulin-like growth factor 1. Exp Cell Res. 1988;174(2):448-60doi: 10.1016/0014-4827(88)90314-x.
Deeks, S., Richards, J., & Nandi, S. (1988). Maintenance of normal rat mammary epithelial cells by insulin and insulin-like growth factor 1. Experimental cell research, 174(2), 448-60. https://doi.org/10.1016/0014-4827(88)90314-x
Deeks, S, et al. "Maintenance of normal rat mammary epithelial cells by insulin and insulin-like growth factor 1." Experimental cell research vol. 174,2 (1988): 448-60. doi: https://doi.org/10.1016/0014-4827(88)90314-x
Deeks S, Richards J, Nandi S. Maintenance of normal rat mammary epithelial cells by insulin and insulin-like growth factor 1. Exp Cell Res. 1988 Feb;174(2):448-60. doi: 10.1016/0014-4827(88)90314-x. PMID: 3276539.
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Exp Cell Res. 1988 Feb;174(2):448-60. doi: 10.1016/0014-4827(88)90314-x.

Maintenance of normal rat mammary epithelial cells by insulin and insulin-like growth factor 1.

Experimental cell research

S Deeks, J Richards, S Nandi

Affiliations

  1. Cancer Research Laboratory, University of California, Berkeley 94720.

PMID: 3276539 DOI: 10.1016/0014-4827(88)90314-x

Abstract

Normal rat mammary epithelial cells were cultured within a rat tail collagen gel matrix formed under improved conditions for controlling pH and osmolarity. Under these conditions, growth can be maintained for up to 3 weeks with a 10- to 15-fold increase in cell number. The cells grow in response to prolactin, progesterone, epidermal growth factor, and cholera toxin, in a medium of DME: Ham's F12 supplemented with BSA and insulin at 10 micrograms/ml. When the insulin concentration was reduced to more physiological levels (10 ng/ml) the cells did not grow. However, at these more physiological concentrations it could be shown that insulin had a concentration-dependent effect on the maintenance of the cells with an optimum concentration around 25 ng/ml. The cells could be maintained in hormone-supplemented medium with low levels of insulin in a quiescent state for up to 14 days. The high levels of insulin needed for optimal growth could be replaced by insulin-like growth factor 1 (IGF-1) at much lower concentrations (25-50 ng/ml). The superphysiological level of insulin required for optimum growth is probably due to its acting weakly through an IGF-1-mediated growth-promoting mechanism. Insulin's effect on cell maintenance occurs at physiological levels and may better reflect its role in mammary cell growth.

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