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de Almeida RF, Fernandes M, de Godoy LMF. An updated map of Trypanosoma cruzi histone post-translational modifications. Sci Data. 2021;8(1):93doi: 10.1038/s41597-021-00818-w.
de Almeida, R. F., Fernandes, M., & de Godoy, L. M. F. (2021). An updated map of Trypanosoma cruzi histone post-translational modifications. Scientific data, 8(1), 93. https://doi.org/10.1038/s41597-021-00818-w
de Almeida, Rafael Fogaça, et al. "An updated map of Trypanosoma cruzi histone post-translational modifications." Scientific data vol. 8,1 (2021): 93. doi: https://doi.org/10.1038/s41597-021-00818-w
de Almeida RF, Fernandes M, de Godoy LMF. An updated map of Trypanosoma cruzi histone post-translational modifications. Sci Data. 2021 Mar 25;8(1):93. doi: 10.1038/s41597-021-00818-w. PMID: 33767201; PMCID: PMC7994815.
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Sci Data. 2021 Mar 25;8(1):93. doi: 10.1038/s41597-021-00818-w.

An updated map of Trypanosoma cruzi histone post-translational modifications.

Scientific data

Rafael Fogaça de Almeida, Matheus Fernandes, Lyris Martins Franco de Godoy

Affiliations

  1. Instituto Carlos Chagas, Fiocruz Parana, Curitiba, Parana, Brazil.
  2. Instituto Carlos Chagas, Fiocruz Parana, Curitiba, Parana, Brazil. [email protected].

PMID: 33767201 PMCID: PMC7994815 DOI: 10.1038/s41597-021-00818-w

Abstract

In humans and other eukaryotes, histone post-translational modifications (hPTMs) play an essential role in the epigenetic control of gene expression. In trypanosomatid parasites, conversely, gene regulation occurs mainly at the post-transcriptional level. However, our group has recently shown that hPTMs are abundant and varied in Trypanosoma cruzi, the etiological agent of Chagas Disease, signaling for possible conserved epigenetic functions. Here, we applied an optimized mass spectrometry-based proteomic workflow to provide a high-confidence comprehensive map of hPTMs, distributed in all canonical, variant and linker histones of T. cruzi. Our work expands the number of known T. cruzi hPTMs by almost 2-fold, representing the largest dataset of hPTMs available to any trypanosomatid to date, and can be used as a basis for functional studies on the dynamic regulation of chromatin by epigenetic mechanisms and the selection of candidates for the development of epigenetic drugs against trypanosomatids.

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