Display options
Cite
Szymkowiak C, Reynolds RL, Chamberlin MJ, et al. The tRNAGlu2 gene in the rrnB operon of E. coli is a prerequisite for correct RNase III processing in vitro. Nucleic Acids Res. 1988;16(16):7885-99doi: 10.1093/nar/16.16.7885.
Szymkowiak, C., Reynolds, R. L., Chamberlin, M. J., & Wagner, R. (1988). The tRNAGlu2 gene in the rrnB operon of E. coli is a prerequisite for correct RNase III processing in vitro. Nucleic acids research, 16(16), 7885-99. https://doi.org/10.1093/nar/16.16.7885
Szymkowiak, C, et al. "The tRNAGlu2 gene in the rrnB operon of E. coli is a prerequisite for correct RNase III processing in vitro." Nucleic acids research vol. 16,16 (1988): 7885-99. doi: https://doi.org/10.1093/nar/16.16.7885
Szymkowiak C, Reynolds RL, Chamberlin MJ, Wagner R. The tRNAGlu2 gene in the rrnB operon of E. coli is a prerequisite for correct RNase III processing in vitro. Nucleic Acids Res. 1988 Aug 25;16(16):7885-99. doi: 10.1093/nar/16.16.7885. PMID: 3047674; PMCID: PMC338498.
Copy Download .nbib Format: NLM
Share it on
Full text links
Silverchair Information Systems Free PMC Article

Nucleic Acids Res. 1988 Aug 25;16(16):7885-99. doi: 10.1093/nar/16.16.7885.

The tRNAGlu2 gene in the rrnB operon of E. coli is a prerequisite for correct RNase III processing in vitro.

Nucleic acids research

C Szymkowiak, R L Reynolds, M J Chamberlin, R Wagner

Affiliations

  1. Max-Planck-Institut für Molekulare Genetik, Abteilung Wittmann, Berlin, FRG.

PMID: 3047674 PMCID: PMC338498 DOI: 10.1093/nar/16.16.7885
Free PMC Article

Abstract

RNase III cleaves precursor 16S RNA and precursor 23S RNA from the ribosomal RNA transcript. In vitro transcription experiments, using plasmids with the rrnB operon truncated in the 16S RNA and with various deletions in the spacer tRNA region, showed that no matter what size of deletion if the tRNA gene was affected RNase III processing of 16S RNA became incomplete. In comparison to a control plasmid, where only the 16S RNA gene was truncated and that showed normal RNA processing, plasmids where the tRNA gene was deleted partially or totally either the 5' or the 3' end of 16S RNA was processed. This relation between RNase III processing and the 3-dimensional structure of tRNA suggests an interaction between RNase III and a tRNA processing enzyme most probably RNase P.

Similar articles

References

  1. J Mol Biol. 1966 Mar;16(1):67-84 - PubMed
  2. J Bacteriol. 1975 Oct;124(1):307-16 - PubMed
  3. Biochemistry. 1975 Oct 21;14(21):4634-8 - PubMed
  4. Arch Biochem Biophys. 1977 Aug;182(2):404-8 - PubMed
  5. Nucleic Acids Res. 1977 Aug;4(8):2527-38 - PubMed
  6. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3593-7 - PubMed
  7. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3598-602 - PubMed
  8. Cell. 1978 Oct;15(2):527-39 - PubMed
  9. Nucleic Acids Res. 1978 Oct;5(10):3665-77 - PubMed
  10. Methods Enzymol. 1979;68:245-67 - PubMed
  11. J Mol Biol. 1981 May 15;148(2):107-27 - PubMed
  12. Microbiol Rev. 1981 Dec;45(4):502-41 - PubMed
  13. Biochem Biophys Res Commun. 1981 Nov 30;103(2):543-51 - PubMed
  14. Proc Natl Acad Sci U S A. 1981 Dec;78(12):7370-4 - PubMed
  15. Cell. 1982 Oct;30(3):669-72 - PubMed
  16. J Biol Chem. 1983 Oct 10;258(19):12034-42 - PubMed
  17. Cell. 1983 Dec;35(3 Pt 2):849-57 - PubMed
  18. Proc Natl Acad Sci U S A. 1984 Jan;81(1):185-8 - PubMed
  19. Biochemistry. 1984 Jun 19;23(13):2952-7 - PubMed
  20. Annu Rev Biochem. 1984;53:75-117 - PubMed
  21. J Mol Biol. 1984 Sep 15;178(2):303-22 - PubMed
  22. J Bacteriol. 1986 Oct;168(1):1-5 - PubMed
  23. FEBS Lett. 1987 Aug 17;220(2):283-7 - PubMed
  24. J Mol Biol. 1987 Jul 5;196(1):85-100 - PubMed
  25. Science. 1988 Jan 8;239(4836):178-81 - PubMed
  26. Mol Microbiol. 1987 Nov;1(3):327-34 - PubMed

Substances

MeSH terms

Publication Types

LinkOut - more resources