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Williams TM, Eisenberg L, Burlein JE, et al. Two regions within the human IL-2 gene promoter are important for inducible IL-2 expression. J Immunol. 1988;141(2):662-6
Williams, T. M., Eisenberg, L., Burlein, J. E., Norris, C. A., Pancer, S., Yao, D., Burger, S., Kamoun, M., & Kant, J. A. (1988). Two regions within the human IL-2 gene promoter are important for inducible IL-2 expression. Journal of immunology (Baltimore, Md. : 1950), 141(2), 662-6.
Williams, T M, et al. "Two regions within the human IL-2 gene promoter are important for inducible IL-2 expression." Journal of immunology (Baltimore, Md. : 1950) vol. 141,2 (1988): 662-6.
Williams TM, Eisenberg L, Burlein JE, Norris CA, Pancer S, Yao D, Burger S, Kamoun M, Kant JA. Two regions within the human IL-2 gene promoter are important for inducible IL-2 expression. J Immunol. 1988 Jul 15;141(2):662-6. PMID: 2838551.
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J Immunol. 1988 Jul 15;141(2):662-6.

Two regions within the human IL-2 gene promoter are important for inducible IL-2 expression.

Journal of immunology (Baltimore, Md. : 1950)

T M Williams, L Eisenberg, J E Burlein, C A Norris, S Pancer, D Yao, S Burger, M Kamoun, J A Kant

Affiliations

  1. Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia 19104.

PMID: 2838551

Abstract

We have examined regulatory domains of the human IL-2 gene promoter by transfection and transient expression of rDNA constructs in which the chloramphenicol acetyl transferase gene shows T cell-specific inducible expression and cyclosporin A-mediated inhibition when placed downstream of 587 bp of the human IL-2 5'-flanking region. A series of 5'-deletion constructs transfected into the Jurkat T lymphoid line demonstrates that a region encompassing 370 bp 5' of the transcription start site is sufficient for inducible chloramphenicol acetyl-transferase expression. Further dissection of this region with internal deletion (linker-scanner) mutants revealed that portions of at least two discrete regions from -42 to -169 and -289 to -361 bp relative to the transcription start site are critical for inducible expression of the IL-2 gene. T cell-specific expression of wild-type and mutant IL-2 promoter constructs could be increased severalfold by the insertion of an upstream SV40 enhancer. With use of a battery of IL-2 promoter constructs, we could not identify subregions within IL-2 5'-flanking sequences which are crucial for cyclosporin A inhibition of the IL-2 gene or deletion of which resulted in loss of T cell-specific expression, suggesting that such functions may be mediated at pre-transcriptional levels.

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