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Elsevier Science

Cancer Genet Cytogenet. 1988 Jul 01;33(1):83-92. doi: 10.1016/0165-4608(88)90053-2.

myb oncogene in human hematopoietic neoplasia with 6q- anomaly.

Cancer genetics and cytogenetics

K Ohyashiki, J H Ohyashiki, A J Kinniburgh, K Toyama, H Ito, J Minowada, A A Sandberg

Affiliations

  1. Department of Internal Medicine, Tokyo Medical College, Japan.

PMID: 2838159 DOI: 10.1016/0165-4608(88)90053-2

Abstract

Molecular and cytogenetic analyses were performed on human T-cell leukemia cell lines (PEER and MOLT-4) with the 6q- anomaly. The PEER cells contained an interstitial deletion of the long arm of chromosome 6, that is, del(6)(q13q21), as well as other changes. The MOLT-4 cells showed a terminal deletion of the long arm of chromosome 6, that is, del(6)(q24). The 700-bp BamHI/XbaI-digested c-myb probe hybridized to a 4.3-kb fragment in EcoRI digested DNAs from these two cell lines, showing no deletion, rearrangement, or amplification. On the other hand, ML cells [ML-1, -2 and -3; human myeloid/T-cell biphenotypic leukemia cell lines with del(6)(q24)] showed an amplification of the c-myb gene and had a high level of the c-myb-related mRNA at 3.5 kb. Though no amplification of the c-myb at the DNA level was noted in the PEER or MOLT-4 cell lines, apparent high expression of the c-myb was detected in these human T-cell neoplastic lines. These results indicate that high c-myb expression is related to lineage of hematopoietic neoplasia rather than to the 6q- change.

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