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Dike LE, Farmer SR. Cell adhesion induces expression of growth-associated genes in suspension-arrested fibroblasts. Proc Natl Acad Sci U S A. 1988;85(18):6792-6doi: 10.1073/pnas.85.18.6792.
Dike, L. E., & Farmer, S. R. (1988). Cell adhesion induces expression of growth-associated genes in suspension-arrested fibroblasts. Proceedings of the National Academy of Sciences of the United States of America, 85(18), 6792-6. https://doi.org/10.1073/pnas.85.18.6792
Dike, L E, and Farmer, S R. "Cell adhesion induces expression of growth-associated genes in suspension-arrested fibroblasts." Proceedings of the National Academy of Sciences of the United States of America vol. 85,18 (1988): 6792-6. doi: https://doi.org/10.1073/pnas.85.18.6792
Dike LE, Farmer SR. Cell adhesion induces expression of growth-associated genes in suspension-arrested fibroblasts. Proc Natl Acad Sci U S A. 1988 Sep;85(18):6792-6. doi: 10.1073/pnas.85.18.6792. PMID: 3045824; PMCID: PMC282064.
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Proc Natl Acad Sci U S A. 1988 Sep;85(18):6792-6. doi: 10.1073/pnas.85.18.6792.

Cell adhesion induces expression of growth-associated genes in suspension-arrested fibroblasts.

Proceedings of the National Academy of Sciences of the United States of America

L E Dike, S R Farmer

Affiliations

  1. Department of Biochemistry, Boston University School of Medicine, MA 02118.

PMID: 3045824 PMCID: PMC282064 DOI: 10.1073/pnas.85.18.6792
Free PMC Article

Abstract

A methylcellulose suspension system that prevents cell-surface contact with the substrate was used to study the role of cell adhesion in the regulation of proliferation. The nonadhesive conditions established by suspension culture cause BALB/c 3T3 (A31) cells to enter a G0 state of growth arrest within 48 hr as defined by an inhibition of DNA synthesis and a suppression of c-myc and histone mRNA expression. The adhesion of these suspension-arrested cells rapidly induces c-fos, c-myc, and actin gene expression. This stimulation did not depend on the presence of serum since the adhesion of suspension-arrested cells, in the absence of serum, also induced the expression of c-fos and c-myc mRNAs. In addition, adhesion onto fibronectin increased the number of cells able to respond to epidermal growth factor and insulin and progress into S phase. These results indicate that adhesion of suspension-arrested cells activates the G0/G1 transition independent of growth factors.

Cited by

Hirsch C, Schildknecht S.
Front Pharmacol. 2019 Dec 10;10:1484. doi: 10.3389/fphar.2019.01484. eCollection 2019.
PMID: 31920667

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