Afr J Prim Health Care Fam Med. 2020 Jun 17;12(1):e1-e8. doi: 10.4102/phcfm.v12i1.2212.
Sensitivity and specificity of malaria rapid diagnostic test (mRDT CareStatTM) compared with microscopy amongst under five children attending a primary care clinic in southern Nigeria.
African journal of primary health care & family medicine
Oluwagbenga Ogunfowokan, Bamidele A Ogunfowokan, Anthony I Nwajei
PMID: 32634015
PMCID: PMC7380062 DOI: 10.4102/phcfm.v12i1.2212
Abstract
BACKGROUND: Malaria diagnosis using microscopy is currently the gold standard. However, malaria rapid diagnostic tests (mRDTs) were developed to simplify the diagnosis in regions without access to functional microscopy.
AIM: The objective of this study was to compare the diagnostic accuracy of mRDT CareStatTM with microscopy.
SETTING: This study was conducted in the paediatric primary care clinic of the Federal Medical Centre, Asaba, Nigeria.
METHODS: A cross-sectional study for diagnostic accuracy was conducted from May 2016 to October 2016. Ninety-eight participants were involved to obtain a precision of 5%, sensitivity of mRDT CareStatTM of 95% from published work and 95% level of confidence after adjusting for 20% non-response rate or missing data. Consecutive participants were tested using both microscopy and mRDT. The results were analysed using EPI Info Version 7.
RESULTS: A total of 98 children aged 3-59 months were enrolled. Malaria prevalence was found to be 53% (95% confidence interval [CI] = 46% - 60%), whilst sensitivity and specificity were 29% (95% CI = 20% - 38%) and 89% (95% CI = 83% - 95%), respectively. The positive and negative predictive values were 75% (95% CI = 66.4% - 83.6%) and 53% (95% CI = 46% - 60%), respectively.
CONCLUSION: Agreement between malaria parasitaemia using microscopy and mRDT positivity increased with increase in the parasite density. The mRDT might be negative when malaria parasite density using microscopy is low.
Keywords: education; family medicine; mRDT; parasite density; primary care; sensitivity; specificity
References
- Malar Res Treat. 2010;2010:858427 - PubMed
- Malar J. 2009 Nov 30;8:271 - PubMed
- Infect Dis Poverty. 2013 Aug 30;2(1):19 - PubMed
- Am J Trop Med Hyg. 2012 Feb;86(2):194-8 - PubMed
- Malar J. 2017 Mar 6;16(1):105 - PubMed
- Malar J. 2014 Mar 17;13:101 - PubMed
- Malar J. 2011 Nov 02;10:332 - PubMed
- Malar J. 2010 Dec 02;9:349 - PubMed
- Clin Infect Dis. 2016 Dec 15;63(suppl 5):S290-S297 - PubMed
- Malar J. 2013 Oct 27;12:374 - PubMed
- Malar J. 2013 Jan 07;12:6 - PubMed
- Parasitol Int. 2007 Sep;56(3):217-20 - PubMed
- Malar J. 2013 May 21;12:167 - PubMed
- Malar J. 2013 May 10;12:159 - PubMed
- Diagn Microbiol Infect Dis. 2010 Feb;66(2):162-8 - PubMed
- J Med Microbiol. 2013 Oct;62(Pt 10):1491-1505 - PubMed
Substances
MeSH terms
Publication Types