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Kim K, Jeon HM, Choi KC, et al. Testing the Effectiveness of . Int J Mol Sci. 2020;21(11)doi: 10.3390/ijms21113898.
Kim, K., Jeon, H. M., Choi, K. C., & Sung, G. Y. (2020). Testing the Effectiveness of . International journal of molecular sciences, 21(11), . https://doi.org/10.3390/ijms21113898
Kim, Kyunghee, et al. "Testing the Effectiveness of ." International journal of molecular sciences vol. 21,11 (2020). doi: https://doi.org/10.3390/ijms21113898
Kim K, Jeon HM, Choi KC, Sung GY. Testing the Effectiveness of . Int J Mol Sci. 2020 May 29;21(11). doi: 10.3390/ijms21113898. PMID: 32486109; PMCID: PMC7312991.
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Int J Mol Sci. 2020 May 29;21(11). doi: 10.3390/ijms21113898.

Testing the Effectiveness of .

International journal of molecular sciences

Kyunghee Kim, Hye Mi Jeon, Kyung Chan Choi, Gun Yong Sung

Affiliations

  1. Cooperative Course of Nano-Medical Device Engineering, Hallym University, Chuncheon 24252, Korea.
  2. Integrative Materials Research Institute, Hallym University, Chuncheon 24252, Korea.
  3. Department of Pathology, Collage of Medicine, Hallym University, Chuncheon 24252, Korea.
  4. Major in Materials Science and Engineering, Hallym University, Chuncheon 24252, Korea.

PMID: 32486109 PMCID: PMC7312991 DOI: 10.3390/ijms21113898

Abstract

The in vitro tests in current research employ simple culture methods that fail to mimic the real human tissue. In this study, we report drug testing with a 'pumpless skin-on-a-chip' that mimics the structural and functional responses of human skin. This model is a skin equivalent constituting two layers of the skin, dermis and epidermis, developed using human primary fibroblasts and keratinocytes. Using the gravity flow device system, the medium was rotated at an angle of 15 degrees on both sides so as to circulate through the pumpless skin-on-a-chip microfluidic channel. This pumpless skin-on-a-chip is composed of upper and lower chips, and is manufactured using porous membranes so that medium can be diffused and supplied to the skin equivalent. Drug testing was performed using Curcuma longa leaf extract (CLLE), a natural product cosmetic ingredient, to evaluate the usefulness of the chip and the efficacy of the cosmetic ingredient. It was found that the skin barrier function of the skin epidermis layer is enhanced to exhibit antiaging effects. This result indicates that the pumpless skin-on-a-chip model can be potentially used not only in the cosmetics and pharmaceutical industries but also in clinical applications as an alternative to animal studies.

Keywords: Curcuma longa; antiaging; human-skin equivalents; microphysiological system; pumpless skin-on-a-chip

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