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Sampson LL, Hendrix RW, Huang WM, et al. Translation initiation controls the relative rates of expression of the bacteriophage lambda late genes. Proc Natl Acad Sci U S A. 1988;85(15):5439-43doi: 10.1073/pnas.85.15.5439.
Sampson, L. L., Hendrix, R. W., Huang, W. M., & Casjens, S. R. (1988). Translation initiation controls the relative rates of expression of the bacteriophage lambda late genes. Proceedings of the National Academy of Sciences of the United States of America, 85(15), 5439-43. https://doi.org/10.1073/pnas.85.15.5439
Sampson, L L, et al. "Translation initiation controls the relative rates of expression of the bacteriophage lambda late genes." Proceedings of the National Academy of Sciences of the United States of America vol. 85,15 (1988): 5439-43. doi: https://doi.org/10.1073/pnas.85.15.5439
Sampson LL, Hendrix RW, Huang WM, Casjens SR. Translation initiation controls the relative rates of expression of the bacteriophage lambda late genes. Proc Natl Acad Sci U S A. 1988 Aug;85(15):5439-43. doi: 10.1073/pnas.85.15.5439. PMID: 2969591; PMCID: PMC281772.
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Proc Natl Acad Sci U S A. 1988 Aug;85(15):5439-43. doi: 10.1073/pnas.85.15.5439.

Translation initiation controls the relative rates of expression of the bacteriophage lambda late genes.

Proceedings of the National Academy of Sciences of the United States of America

L L Sampson, R W Hendrix, W M Huang, S R Casjens

Affiliations

  1. Department of Cellular, Viral and Molecular Biology, University of Utah Medical Center, Salt Lake City 84132.

PMID: 2969591 PMCID: PMC281772 DOI: 10.1073/pnas.85.15.5439
Free PMC Article

Abstract

The late operon of bacteriophage lambda contains the genes encoding the morphogenetic proteins of the phage. These genes are transcribed equally from the single late promoter. Although the functional half-lives of the mRNA for the various genes of this operon vary less than 2-fold, their relative rates of expression have been shown to vary by nearly 1000-fold. This variation could result from differing rates of translation initiation, from overlapping upstream translation, or from differential elongation rates due to the presence of codons for which the corresponding tRNAs are rare. To distinguish between these possibilities, we have cloned sequences surrounding the initiator codons of several of these genes and measured their ability to drive synthesis of hybrid lambda-beta-galactosidase proteins. The rates of expression of the hybrid genes thus produced correlate very well with the natural rates of expression of the corresponding phage genes, suggesting that the rate of initiation of translation controls the relative expression rates of these genes.

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